Immunocytochemical analysis of RAB27A was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-human/mouse/Rat RAB27A Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47907) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the 557-conjugated Anti-Sheep IgG Secondary Antibody (re and counterstained with DAPI (blue). Specific staining was localized to cytoplasm.

Western blot analysis from lysates of K562 human chronic myelogenous leukemia cell line, 786-O human renal cell adenocarcinoma cell line, MCF-7 human breast cancer cell line, Jurkat human acute T cell leukemia cell line, Rat-2 rat embryonic fibroblast cell line, and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-human/mouse/Rat RAB27A Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47907) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for RAB27A at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions.