Cell Signaling
IκBα (44D4) Rabbit mAb
4812
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In our experimental project we sought to determine downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigate possible changes in IKK Complex, which is a regulator of NFkB activation and the effect of LPA on inflammatory transcription factors.
Western Blot
BV2 microglia cells
1:500 overnight at +4
5% milk
1:5000 goat anti-rabbit, 2 hours at room temperature
N/A
ChemiDoc Development System
The expression of IkBa was gradually decreased over time after LPA atreatment.
Easy to optimise and efficient results.
Not a very strong signal in our experiments
Generally a good antibody