IkappaBalpha (44D4) Rabbit mAb 4812S from Cell Signaling Technology

Product Specs
ItemIkappaBalpha (44D4) Rabbit mAb
CompanyCell Signaling Technology
Price $339.00 Inquire
Catalog Number4812S
Quantity100 µl
ApplicationsW, IP
ReactivityH, M, R, Hm, Mk, Mi
TargetIkB-a
HostRabbit
NCBI Gene AliasesEDAID2, IKBA, MAD-3, NFKBI
ClonalityMonoclonal
NCBI Full Gene NameNFKB inhibitor alpha
Research AreaImmunology and Inflammation
Clone44D4
Molecular Weight39

Questions and Answers

Can this antibody be used to study the NF-κB signaling pathway in human cells?

Yes, the IκBα (44D4) Rabbit mAb has been shown to be effective in detecting IκBα levels, which is crucial for understanding the NF-κB signaling pathway. For instance, in the study by , the antibody was utilized to assess the activation of NF-κB in human skin mast cells.

Is this antibody suitable for use in Western blotting applications?

The IκBα (44D4) Rabbit mAb is suitable for Western blotting, as evidenced by its application in various studies, including and . In these studies, the antibody was employed to analyze protein expression levels in different experimental contexts.

What species can this antibody react with?

This antibody has been shown to react with multiple species, including human, mouse, rat, non-human primates, and other mammals. This broad reactivity makes it versatile for various experimental models.

Are there any specific applications where this antibody has been cited in the literature?

Yes, the IκBα (44D4) Rabbit mAb has been cited in studies involving immunoprecipitation and Western blotting, particularly in the context of investigating inflammatory responses and signaling pathways, such as in the research conducted by and .

Cell Signaling Technology

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Phone: 978-867-2388
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Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) A BAI1-PSTB-Hydrogel promotes diabetic wound healing by targeting mtDNA leakage and the cGAS–STING axis to alleviate endothelial senescenceBioactive MaterialsNovember 1, 2026Xianqi Wang, Rui Zheng, Wenjie Cheng, Yanan Xu, Xian-jie Xu, Heliang Fu, Dan Wu, Peiwen Wang, Yang Zhang, Huirong Wu, et al.79978S), STING (CST, 13647S), p-TBK1 (CST, 5483S), TBK1 (CST, 38066S), p-IκB (CST, 2859S), IκB (CST, 4812S), p-NF-κB (CST, 3033S), NF-κB (Proteintech, 80979-1-RR), p-IRF-3 (CST, 2859S), IRF-3 (CST, 4812S). After

(2) Glycine tabacina (Labill.) Benth. Ethanol Extract Attenuates LPS-Induced Neuroinflammation and Behavioral Deficits by Modulating TLR4/NF-κB/NLRP3 Signaling PathwayInt J Mol SciJuly 20, 2026Boyang Qiu, Yichen Zhuo, Zixin Teng, Wendi Yue, Yongkai Liang, Zhimin Miao, Yuxin Zhao, Xiaobing Cui, Yunzhen Gao, Lan Zhang, et al.USA" 5206T,TAK-1,CST 9339S,Phospho-TAK1,CST 61294S,IKKα,CST 2697S,Phospho-IKKα/β,CST 8242S,NF-κB p65,CST 3033S,Phospho-NF-κB p65,CST 4812S,IκBα,CST 2859S,Phospho-IκBα,CST 97166S,GAPDH,CST 13435S,Lamin B1,CST P0131,Anti-fluorescence quenching

(3) Atractylodis Macrocephalae Rhizoma ameliorates diarrhea induced by cold drinks and a high-fat diet by remodeling gut microecology and restoring barrier functionChin MedJuly 15, 2026Yajuan Tian, Chenxing Wang, Yuzhong Du, Jiayi Zheng, Su Gao, Hengpu Zhou, Meiqiu Yan, Jingjing Yu, Bai Tao, Zengguang Gao, et al.(ab40675) and β-actin (ab8226) were purchased from Abcam (Cambridge, UK). Antibodies targeting IκBα (4812), NF-κB p65 (3033), p-IκBα (2859), and p-NF-κB p65 (8242) were acquired from Cell Signaling Technology

(4) IL-17–driven tumor cell–intrinsic inflammatory programming creates an immunotherapy-permissive microenvironmentMolecular CancerJune 30, 2026Kosuke Murakami, Shiki Takamura, Chiho Miyagawa, Shiro Takamatsu, Yoko Kashima, Koji Nagaoka, Yukari Kobayashi, Yoshiyuki Hakata, Shigeki Kato, Sachiyo Tsuji-Kawahara, et al.antibody (clone D14E12, #8242, Cell Signaling Technology, Danvers, MA, USA), IκBα antibody (clone 44D4, #4812, Cell Signaling Technology), phospho-IκBα (Ser36) antibody (clone EPR6235(2), ab133462, Abcam, Cambridge,

(5) Seawater Immersion Hypothermia Triggers Cardiac Pyroptosis via the NF-κB/NLRP3 Inflammasome Axis: A Mechanistic Study in RatsInternational Journal of Molecular SciencesJune 30, 2026Huifang Deng, Chaoyue Sun, Zhibo Wang, Hongbiao Chen, Yiwen Ben, Yukun Wu, Wumu Xu, Jiaqi Wang, Yajing Wang, Yanrong Gong, et al.Immunoway, San Jose, CA, USA, 1:2000), IKK-α/β (PT0435R, Immunoway, San Jose, CA, USA, 1:1000), IκBα (#4812S, Cell Signaling Technology, Danvers, MA, USA, 1:1000), NF-κB p65 (D14E12), (#8242T, Cell Signaling Technology,

(6) Compound 4′-O-methylbroussochalcone B attenuates LPS/ATP-induced macrophage pyroptosis in vitro through modulation of the NLRP3 inflammasome and MAPK/NF-κB pathwaysFrontiers in PharmacologyJune 24, 2026Jihee Choi, You Chul Chung, Ami Lee, Han Na Kang, Youn-Hwan Hwang(#63124), phospho-ERK (#4376), ERK (#4696), phospho-p38 (#4511), p38 (#9212), phospho-IκBα (#2859), IκBα (#4812), NF-κB p65 (#8242), Lamin B1 (#12586), and β-actin (#3700) (all from Cell Signaling Technology, unless

(7) Mycobacterium tuberculosis subverts macrophage-mediated defense through exploitation of the Zn transporter ZIP8iScienceJune 19, 2026Eusondia Arnett, Miranda Lumbreras, Elizabeth Hernandez, Daniela Campos, Chrissy M. Leopold Wager, Susanta Pahari, Dahlia Matouba, Brenden F. Determann, Charles Renshaw, Brandon Longstreet, et al.(Cell Signaling 3033), p65 (Cell Signaling 4764), p-IκBα (Cell Signaling 2859S), IκBα (Cell Signaling 4812S), and β-actin (clone 13E5, cat# 5125 Cell Signaling). The membranes were developed using clarity ECL

(8) Theaflavins counteract free fatty acid-driven oxidative-inflammatory injury in endothelial cells through Nrf2-NF-κB axisFront ImmunolJune 9, 2026Yufei Zhao, Biao QuNrf2 (Cat No. 12721), HO-1(Cat no: 43966), SOD2 (Cat no: 13141), GPX1(Cat no: 3286), IκBα (Cat no: 4812), NF-κB p65 (Cat no: 8242), GAPDH (Cat no: 2118) and Histone H3 (Cat no: 4499)were supplied by Cell Signaling Technology

(9) De novo NFKBIA variants within the N-terminal hotspot: consistent immunophenotype and divergent clinical presentationsFront ImmunolJune 5, 2026Rui Gan, Guangzhao Li, Lina Zhou, Li Wang, Rongxin Dai, Xuemei Tang, Junfeng Wu, Yanjun Jia, Qing Zhou, Xiaodong Zhao, et al.subjected to SDS-PAGE and immunoblotting. Antibodies used were anti-IκBα (Cell Signaling Technology, #4812, 1:1000), anti-vinculin (Proteintech, #66305-1-Ig, 1:1000), anti-α-tubulin (Proteintech, #11224-1-AP,

(10) Urolithin A supplementation alleviates osteogenic disfunction and promotes bone fracture healing in inflammatory environmentsFood & Nutrition ResearchMay 21, 2026Jinwu Bai, Jixing Fan, Ruideng Wang, Shilong Su, Daole Hu, Xi He, Shan Gao, Fang ZhouAntibodies against GAPDH (#2118), RUNX2 (#12556), p-P65 (#3033), P65 (#8242), p-IκBα (#2859), IκBα (#4812), p-JNK (#4668), JNK (#9252), p-ERK (#4370), ERK (#4695), p-P38 (#4511), and P38 (#9212) were purchased

(11) Metformin Attenuates Angiotensin II-Induced Cardiac Inflammaging-Like Injury Through Coordinated Nrf2 Activation and NF-κB SuppressionIranian Journal of Pharmaceutical ResearchMay 20, 2026Huarui Li, Deming Fu, Umar Saeed, Zahra Zahid Piracha, Jianjun Bao(Cat. No. 62262), NF-κB p65 (Cat. No. 8242), phospho-NF-κB p65 (Ser536) (Cat. No. 3033), IκBα (Cat. No. 4812), BAX (Cat. No. 2772), BCL-2 (Cat. No. 4223), cleaved caspase-3 (Asp175) (Cat. No. 9664), cleaved PARP

(12) Targeting ANGPTL3 and IL‐33/ST2 Ameliorates Diabetic Kidney Disease by Reducing Lipotoxicity, Alleviating Inflammation and Inhibiting FibrosisAdvanced ScienceMay 19, 2026Zhuojin Li, Zhonglian Cao, Rongrui Zhou, Xu Cheng, Xiaozhi Hu, Shuwen Xu, Zihan Dou, Ling Du, Dianwen Jurabbit or mouse monoclonal antibodies were employed: NF‐κB p65 (#8242), p‐NF‐κB p65 (#3033), IκBα (#4812), and p‐IκBα (#2859) from Cell Signaling Technology, alongside caspase‐1 (ab179515, Abcam) and GAPDH

(13) Linalool targets NR3C2 to inhibit NF-κB-mediated gastric cancer progressionCell DivisionMay 18, 2026Ying Hu, Shunfeng Chen, Yu Wang, Ling Liu, Chaoyi Zhang, Hong Chen, Yuanyuan Duanmu, Zhaotian Su, Jun MaUSA), p65 (1:200, 51–0500, Invitrogen), Phospho-p65 (1:1000, MA5-37156, Invitrogen), IκBα (1:1000, 4812, CST), Phospho-IκBα (1:1000, 2859, CST), IL-1β (1:1000, 12703, CST), IL-8 (1:1000, 94407, CST), GAPDH

(14) Betrixaban activates cGAS-STING to promote antitumor immunity without pathological inflammationEMBO Molecular MedicineMay 14, 2026Yang Zhao, Xingyu Chen, LiRui Tang, Hanjie Liu, Boming Kang, Shuailong Zheng, Songying Ouyang, Yunfei Xie, Fuping You(D14E12) XP (catalog no. 8242), phospho-IκBα (Ser32) (14D4) (catalog no. 2859), IκBα (44D4) (catalog no. 4812), and phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) (catalog no. 2078), were obtained from Cell Signaling Technology

(15) ASC-Derived Extracellular Vesicles Suppress Macrophage-Driven Inflammatory Amplification and Contractile Activation of Uterine Smooth Muscle CellsInt J Mol SciMay 11, 2026Ji-Seon Lee, You-Rin Kim, Dogeon Yoon, Ji Hye Park, Tae-Keun Kim, Eun-Kyoung Choi, Jun Hur, Ji-Eun Song(#9251), phospho-NF-κB p65 (Ser536) (#3033), NF-κB p65 (#8242), phospho-IκBα (Ser32) (#2859), and IκBα (#4812) were obtained from Cell Signaling Technology (Danvers, MA, USA). Lamin B1 antibody (ab133741) was purchased

(16) Ubc9-mediated SUMOylation of Ninj1 alleviates inflammatory responses in hepatic ischaemia/reperfusion injuryClin Transl MedMay 10, 2026Kang Huang, Li Xu, Shufang Na, Yan Xu, Qiaoyun Liu, Shaojun Ye, Cong-Yi Wang, Wei Zhou, Qifa Ye37705T), p‐Mlkl (CST, 37333T), P65 (Proteintech, 10745‐1‐AP), p‐P65 (Proteintech, 82335‐1‐RR), IκBɑ (CST, 4812T), IKKβ (CST, 8943T), p‐IKKβ (CST, 2078T) and Ninj1 (HUABIO, ER61906). β‐Actin, β‐tubulin and Na+/K+ATPase

(17) Immunomodulatory Effects of Human Breast Milk-Derived Exosomes on Myeloid Cells and ChondrocytesBiomolecules & TherapeuticsMay 1, 2026Dahyeon Kwon, Ji Yoon Yoo, Kang-Bin Dan, Ki-Uk Kim, Ji-Yun Lee, Hyeyoung MinCell signaling Technology), phospho-IκBα (Ser32/36, #9246S, Cell signaling Technology), total IκBα (#4812S, Cell signaling Technology), and GAPDH (#2118S, Cell signaling Technology). Following incubation with

(18) Lactate modulates microglial M2 polarization via H3K9 lactylation in ischemic strokeJournal of Translational Internal MedicineApril 28, 2026Bingwei Li, Kan Xu, Jinlu Yu1:1000), anti-NF-κB p65 (CST, 8242; 1:500), antiphospho-NF-κB p65 (CST, 3033; 1:500), anti-IκBα (CST, 4812; 1: 500), anti-phospho-IκBα (CST, 9246; 1:500), and anti-β-actin (Proteintech, 66009–1; 1:5000). Following

(19) Baicalein Protects H9c2 Cardiomyoblasts Against LPS-Induced Inflammatory Injury by Modulating the NF-κB/NLRP3 Inflammasome Pathway and Mitochondrial ROSIran J Pharm ResApril 28, 2026Yi Lu, Zahra Zahid Piracha, Umar Saeed, Fangjun Luo"Cell Signaling Technology (CST; Danvers, MA, USA)",8242 IκBα,"Cell Signaling Technology (CST; Danvers, MA, USA)",4812 GAPDH,"Cell Signaling Technology (CST; Danvers, MA, USA)",5174 List of Primary Antibodies

(20) ABTB1 modulates EGFR signaling and confers icotinib sensitivity in lung adenocarcinomaRespiratory ResearchApril 27, 2026Si-Yang Li, Lu Xia, Peng Xu, Gen-hong Li, Bing-chun Yang, Jing-Xun Wu, Fu-Xing Zhang, Jia-Bian Lian(Tyr1068) (Cat# AP0301) (ABclonal, Wuhan, China); STAT3 (Cat# 9139), phospho-STAT3 (Cat# 9145), IκB (Cat# 4812), phospho-IκB (Cat# 2859), p65 (Cat# 8242), phospho-p65 (Cat# 3033) (Cell Signaling Technology, Danvers,

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1/5/2021Detection of Changes in IkBa Expression Lisha JoshiIn our experimental project we sought to determine downstream signaling pathways that are activated by lysophosphatidic acid (LPA). We investigate possible changes in IKK Complex, which is a regulator... Read Review
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8/3/2015Review for Rabbit Anti–IkB-a Antibody Liyong Z.Protein extract from cells was prepared in radioimmunoprecipitation assay lysis buffer (sc-24948; Santa Cruz Biotechnology) supplied with PMSF, protease inhibitor mixture, sodium orthovanadate, or ... Read Review

IkappaBalpha (44D4) Rabbit mAb

IkappaBalpha (44D4) Rabbit mAb from Cell Signaling Technology

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Cell Signaling Technology

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IkappaBalpha (44D4) Rabbit mAb from Cell Signaling Technology

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