Great Staining for Mitochondria

Johns Hopkins School of Medicine
Oncology
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
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Company:

Thermo Fisher Scientific

Product Name:

MitoTracker™ Deep Red FM

Catalog Number:

M22426

We are interested in studying the mechanism of T cell differentiation through modulating cellular metabolism. This dye is very bright.

Experimental Design and Results Summary

Application

Flow Cytometry

Starting Material

Mouse lymphocytes

Tips

Need to titrate

Results Summary

The dye can be too bright. Needs to optimize for flow staining. Also able to detect differences among different treatment conditions.

DOI or PMID #

N/A

Image Gallery

Summary

The Good

Very bright and can be fixed with PFA

The Bad

Can overspill to nearby channel on flow cytometer

The Bottom Line

Prefer this over MitoTracker Green since this one can be fixed.

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