A Probe for Mitochondrial Superoxide

April 22, 2019

BlueskyReddit
University of Birmingham
ICGS
Graduate Student

Overall

Quality of Results

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Company:

Invitrogen

Product Name:

MitoSox

Catalog Number:

M36008

Easy-to-use fluorescent probe, binding to mitochondrial superoxide in live cells. Requires a brief incubation of 15 mins at 37 degrees after which you can detect the mitochondrial superoxide using a plate reader.

Experimental Design and Results Summary

Application

To detect mitochondrial superoxide in various cell lines

Starting Material

Different cell lines, a plate reader, Krebs medium, CaCl

Tips

Make sure to perform the protein assay in duplicate

Results Summary

Easy-to-perform assay, although one needs to be careful with data analysis. Its good to create an excel template for data analysis

DOI or PMID #

N/A

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Summary

The Good

Quite sensitive assay

The Bad

Data analysis might take some time

The Bottom Line

Once everything is prepared for the assay (Krebs medium could be pre-made, aliquoted and stored at -20), it is a relatively easy experiment to perform. Whilst cells are in the plate reader, make sure to prepare everything for the protein assay (sufficient volume of Bradford reagent, etc.). Overall, it's a precise probe to use for the detection of mitochondrial superoxide.

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