Cell Signaling Technology
Cyclin D1 (92G2) Rabbit mAb
2978
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Cyclin D1 Western Blot Antibodies
This study seeks to validate functional genomic mutations observed in human cancer cells resistant to drug therapies. This antibody was used in Western blotting of human cancer cell lysates.
Western Blot
Human cancer cell lysates
1:1000 in 5% non-fat milk in TBS-T, overnight, 4 degrees Celsius
5% non-fat milk in TBS-T, 1 hour, RT
1:10,000 (anti-Ms) in 5% non-fat milk in TBS-T, 30 minutes, RT
N/A
Chemiluminescence HRP substrate
We observed bands at approximately 35 kDa, very near to the predicted size of Cyclin D1 (36 kDa). There are also very large non-specific bands near the top of the gels - these may be an artifact of the electrophoresis procedure and unrelated to the specificity of the antibody.
Recognizes band corresponding to the correct predicted size of Cyclin D1
Observed non-specific bands
Overall, this is a decent antibody that I would purchase and use again.