Cell Signaling Technology
Snail (C15D3) Rabbit mAb
3879
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We have identified a class of ATPase inhibitors that show promise for treatment of ovarian cancer. We are currently evaluating what mechanisms, if any, may lead to resistance to theses ATPase inhibitors. We are measuring changes in protein expression in an animal model of drug resistance. I chose this product because of CST's reputation for reliable antibodies.
Western Blot
Cellular ascites from PDX mouse models of resistance (lysates)
1:1000 dilution in 5% milk in TBS-T, overnight at 4 degrees C
5% milk in TBS-T
1:10,000 in 5% milk in TBS-T, 30 minutes at RT
N/A
Standard ECL solution (Pierce), 1 minute incubation
Good bands, with some background. Overall there are clean, single bands observed.
Good clean bands
Nothing!
Good antibody, would recommend.