Dissociate Tissues for Cell Recovery

Dana Farber Cancer Institute
Cancer Immunology & AIDS
Research Fellow

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Company:

Miltenyi Biotec

Product Name:

gentleMACS Dissociator

Catalog Number:

130-093-235

This is basically a glorified blender that dissociates any tissue digested with collagenase, to extract infiltrating cells. It can be used for tumors, pancreases, kidneys, heart etc. Some solid tissues are difficult to dissociate by manual agitation, and this is where it comes handy. Plus, who wants to hurt their hands when you have a million tissues to process? We actually performed a yield test and found that tumors dissociated with this instrument gave 5 times higher numbers of infiltrating leukocytes than manually agitated samples.

Experimental Design and Results Summary

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Summary

The Good

High yield of infiltrating cells

The Bad

High price, proprietary sale of C tubes by the company

The Bottom Line

It's a great instrument for isolating tumor infiltrating leukocytes, but overpriced.

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