
Purification of nucleic acids is a multi-step process, beginning from cellular lysis to the collection of the final DNA or RNA product. In these workflows, various stand-alone accessory reagents may be used to enhance the amount yield or purity of the final product. Among these include specialized cell lysis kits, buffers and enzymes (like lysozyme) which can ensure the effective release of nucleic acid from cellular compartments. For removing contaminants, endotoxin removal reagents can be useful for samples susceptible to bacterial lipopolysaccharide contamination, such as in plasmid DNA preps. Nucleases such as DNases and RNases are useful for eliminating any unwanted residual nucleic acids from RNA or DNA preps. In certain extraction procedures such as ethanol precipitation, the optional addition of glycogen can enhance the formation of the final pellet.
Tips on how to mitigate damage and extract useful DNA
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Tips on how to achieve reliable results in molecular biology workflows
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Viagen DirectPCR Lysis Reagent is a buffer used to lyse animal tissue and release the DNA for future applications, such as PCR. It promises to contain inhibitors of...
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This kit is used for isolating high-molecular-weight DNA from H9 stem cell samples for long reading sequencing. Following the protocol, the procedure is easy and fast and yields high purity and high molecular weight of DNA for further use.
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