Antibody Pair

Antibody pairs are commonly used in sandwich ELISA assays, where one antibody (capture antibody) binds to the target antigen, and a second antibody (detection antibody) binds to a different epitope on the antigen. Using matched antibody pairs ensures that the detection is highly specific to the target antigen, reducing the likelihood of cross-reactivity with other proteins. They also enable quantitative measurement of antigen levels in various sample types, such as serum, plasma, and cell culture supernatants. Pre-matched antibody pairs simplify the assay development process, as researchers do not need to spend time optimizing antibody combinations. When selecting antibodies, carefully evaluate within the supplier datasheet if the product is validated for the intended application, reactive to the appropriate species, and if any cross-reactivity has been noted. This setup allows for highly specific and sensitive detection of the target protein. Check if the antibody pair has been validated for the specific application you intend to use, such as ELISA