Fig 1: ELISA confirms gene expression data in Fig. 4. Ldlr-/- mice were fed the four diets, and the levels of protein in jejunum mucus (except for osteopontin, which was determined in whole jejunum) were determined by ELISA as described in Materials and methods. The gender, age, and number of mice per group for each protein are shown in supplemental Table S3. A: Regenerating islet-derived family member 3 alpha (REG3A). B: Regenerating islet-derived family member 3 beta (REG3B). C: Lysozyme. D: Mucin 2 (MUC2). E: ApoA-I. F: Surfactant A. G: LPS-binding protein (LBP). H: Osteopontin. I: Immunoglobulin A (IgA). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.