Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(781) Two MC1R loss-of-function alleles in cream-coloured Australian Cattle Dogs and white HuskiesAnimal GeneticsAugust 1, 2018N. DÃ¼rig, A. Letko, V. Lepori, S. Hadji Rasouliha, R. Loechel, A. Kehl, M. K. HytÃ¶nen, H. Lohi, N. Mauri, J. Dietrich, et al.DNA was extracted from EDTA blood samples using the Maxwell RSC Whole Blood DNA kit, used with the Maxwell RSC Instrument (Promega). Samples from 50 Huskies were used. These were either Siberian Huskies, an AKC and FCI recognized

(782) Circular RNA CpG island hypermethylation-associated silencing in human cancerOncotargetJune 26, 2018Ferreira HJ, Davalos V, de Moura MC, Soler M, Perez-Salvia M, Bueno-Costa A, Setien F, Moran S, Villanueva A, Esteller M.DKO were purified using the Maxwell® RSC miRNA Tissue Kit (Cat. #AS1460, Promega) in the automated Maxwell® RSC Instrument (Promega), according to the manufacturer’s instructions. Human colon total RNA was obtained from Ambion,

(783) Combining amplicon sequencing and metabolomics in cirrhotic patients highlights distinctive microbiota features involved in bacterial translocation, systemic inflammation and hepatic encephalopathyScientific reportsMay 29, 2018Iebba V, Guerrieri F, Di Gregorio V, Levrero M, Gagliardi A, Santangelo F, Sobolev AP, Circi S, Giannelli V, Mannina L, et al.biopsies (15 mg each) and from peripheral/portal blood (200 μl each) was automatically extracted with a Maxwell® RSC Instrument (Promega, Wisconsin, USA, kit #AS1400). For all three sample types, the manufacturer’s protocol was

(784) Intron retention and nuclear loss of SFPQ are molecular hallmarks of ALSNature communicationsMay 22, 2018Luisier R, Tyzack GE, Hall CE, Mitchell JS, Devine H, Taha DM, Malik B, Meyer I, Greensmith L, Newcombe J, et al.and sequencing The Promega Maxwell RSC simplyRNA cells kit including DNase treatment, alongside the Maxwell RSC instrument, was used for RNA extractions. For qPCR validations, RNA was extracted using the RNeasy Plus Mini Kit

(785) First case of human infection with Plasmodium knowlesi in LaosPLOS Neglected Tropical DiseasesMarch 23, 2018Moritoshi Iwagami, Masami Nakatsu, Phonepadith Khattignavong, Pheovaly Soundala, Lavy Lorphachan, Sengdeuane Keomalaphet, Phonepadith Xangsayalath, Satoru Kawai, Bouasy Hongvanthong, Paul T. Brey, et al.22, 2018 1/5 DNA analysis DNA was extracted from the dried blood spot on the filter paper with a Maxwell RSC Instrument (Promega, United States of America) in accordance with the manufacturer’s instructions with minor modification

(786) Functional genomics of lipid metabolism in the oleaginous yeast Rhodosporidium toruloideseLifeMarch 9, 2018Samuel T Coradetti et al.a Maxwell RSC Plant RNA Kit (Promega, AS1500, Madison, WI) using a Maxwell RSC instrument (Promega, AS4500). RNA was sequenced and mapped to the R. toruloides IFO 0880 genome at the Department of Energy Joint

(787) A novel PCR-based system for the detection of four species of human malaria parasites and Plasmodium knowlesiPLOS ONEJanuary 25, 2018Kanako Komaki-Yasuda, Jeanne PerpÃ©tue Vincent, Masami Nakatsu, Yasuyuki Kato, Norio Ohmagari, Shigeyuki KanoQIAamp DNA Mini Kit (Qiagen, Venlo, Netherlands). We also performed automated DNA extraction using a Maxwell RSC Instrument (Promega, Madison, WI, USA). After the purification step, the DNA samples were eluted with 50 μL or

(788) Prognostic value of quantitative ctDNA levels in non small cell lung cancer patientsOncotargetJanuary 2, 2018Mariano Provencio, MarÃa Torrente, Virginia Calvo, David PÃ©rez-Callejo, Lourdes GutiÃ©rrez, Fernando Franco, Clara PÃ©rez-Barrios, Miguel BarquÃn, Ana Royuela, Francisco GarcÃa-GarcÃa, et al.previously described [5, 15]. cfDNA was extracted using a starting volume of 1 ml of plasma with a Maxwell® RSC instrument (Promega), using the Maxwell® RSC ccfDNA Plasma Kit, as specified by the manufacturer and was eluted

(789) Periparturient lipolysis and oxylipid biosynthesis in bovine adipose tissuesPLOS ONEDecember 5, 2017G. Andres Contreras, Clarissa Strieder-Barboza, Jonas de Souza, Jeff Gandy, Vengai Mavangira, Adam L. Lock, Lorraine M. SordilloGene expression analysis RNA was extracted from AT samples using the Maxwell® RSC simplyRNA Tissue Kit (Cat# AS1390) and a Maxwell® RSC Instrument according to manufacturer instructions (Promega, Madison, WI). In brief, 50 mg of subcutaneous AT sample

(790) Damage-induced lncRNAs control the DNA damage response through interaction with DDRNAs at individual double-strand breaksNature cell biologyDecember 1, 2017Michelini F, Pitchiaya S, Vitelli V, Sharma S, Gioia U, Pessina F, Cabrini M, Wang Y, Capozzo I, Iannelli F, et al.RNA. For gel-extraction experiments, total RNA was purified using the Maxwell® miRNA Tissue Kit with the Maxwell® RSC Instrument (Promega) and loaded onto 10% Urea-PAGE. For gel-extracted small RNA analysis, a C. elegans miRNA was

(791) Circulating Cell-free DNA for Metastatic Cervical Cancer Detection, Genotyping, and MonitoringClinical Cancer ResearchNovember 15, 2017Zhigang Kang, Sanja StevanoviÄ‡, Christian S. Hinrichs, Liang CaoHPV ccfDNA isolation and ddPCR analysis The ccfDNA was extracted from 1 mL of sera with an automated Maxwell RSC Instrument (Promega) using a cellulose magnet resin–based ccfDNA purification process. Briefly, serum or plasma

(792) OCA2 splice site variant in German Spitz dogs with oculocutaneous albinismPLOS ONEOctober 3, 2017Madleina Caduff, Anina Bauer, Vidhya Jagannathan, Tosso LeebSpitz litter (Giant Spitz variety). We isolated genomic DNA with the Maxwell RSC Whole Blood DNA Kit and the Maxwell RSC Instrument (Promega). Genotyping of the two parents and six littermates was performed by GeneSeek/Neogen on the

(793) Dynamic circulating tumor DNA quantificaton for the individualization of non-small-cell lung cancer patients treatmentOncotargetSeptember 1, 2017Mariano Provencio, MarÃa Torrente, Virgina Calvo, Lourdes GutiÃ©rrez, David PÃ©rez-Callejo, Clara PÃ©rez-Barrios, Miguel BarquÃn, Ana Royuela, BegoÃ±a Rodriguez-Alfonso, Miguel Sotelo, et al.previously described [14, 25]. cfDNA was extracted using a starting volume of 1 ml of plasma with a Maxwell® RSC instrument (Promega), using the Maxwell® RSC ccfDNA Plasma Kit, as specified by the manufacturer and was eluted

(794) A Large Deletion in the NSDHL Gene in Labrador Retrievers with a Congenital Cornification DisorderG3 Genes|Genomes|GeneticsSeptember 1, 2017Anina Bauer, Michela De Lucia, Vidhya Jagannathan, Giorgia Mezzalira, Margret L. Casal, Monika M. Welle, Tosso Leebrevision. DNA isolation Genomic DNA was isolated from EDTA blood of the two affected dogs using a Maxwell RSC instrument (Promega). We additionally used DNA from EDTA blood of 29 female and 21 male nonaffected control Labrador

(795) A SINE Insertion in ATP1B2 in Belgian Shepherd Dogs Affected by Spongy Degeneration with Cerebellar Ataxia (SDCA2)G3 (Bethesda, Md.)August 7, 2017Mauri N, Kleiter M, Dietschi E, Leschnik M, Högler S, Wiedmer M, Dietrich J, Henke D, Steffen F, Schuller S, et al.isolated from EDTA blood samples with the Maxwell RSC Whole Blood DNA Kit, which were used with the Maxwell RSC Instrument (Promega). Genotyping was done on Illumina CanineHD Chips containing 173,662 genome-wide SNPs by GeneSeek/Neogen.

(796) Subclonal analysis in a lobular breast cancer with classical and solid growth pattern mimicking a solid-papillary carcinomaThe Journal of Pathology: Clinical ResearchJuly 19, 2017Matthias Christgen, Stephan Bartels, Jana Lisa van Luttikhuizen, Maximilian Schieck, Stefanie Pertschy, Sudip Kundu, Ulrich Lehmann, Bjoern Sander, Enrico Pelz, Florian LÃ¤nger, et al.Subsequently, DNA and RNA were extracted with the Maxwell RSC DNA FFPE kit (Promega, Madison, WI, USA) on a Maxwell RSC instrument according to the manufacturer's recommendations. DNA and RNA were quantified using a Qubit 2.0 fluorometer

(797) Whole-exome sequencing in evaluation of patients with venous thromboembolismBlood AdvancesJuly 11, 2017Eun-Ju Lee, Daniel J. Dykas, Andrew D. Leavitt, Rodney M. Camire, Eduard Ebberink, Pablo GarcÃa de Frutos, Kavitha Gnanasambandan, Sean X. Gu, James A. Huntington, Steven R. Lentz, et al.panel Venous blood was collected in a potassium EDTA tube and genomic DNA purified for WES using the Maxwell RSC Instrument (standard instrument protocol; Promega Corp). DNA fragments containing targeted coding sequences were

(798) A highly specific and sensitive massive parallel sequencer-based test for somatic mutations in non-small cell lung cancerPLOS ONEApril 27, 2017Yoshiaki Inoue, Jun Shiihara, Hitoshi Miyazawa, Hiromitsu Ohta, Megumi Higo, Yoshiaki Nagai, Kunihiko Kobayashi, Yasuo Saijo, Masanori Tsuchida, Mitsuo Nakayama, et al.confirmation of cancer cells in the samples are shown in S1 Fig [9, 14]. DNA and RNA were isolated using a Maxwell RSC Instrument (Promega, Madison, WI, USA). Human genomic DNA isolated from immortalized B lymphocyte cell lines established

(799) Glutamine activates STAT3 to control cancer cell proliferation independently of glutamine metabolismOncogeneApril 1, 2017Andrea Cacace, Martina Sboarina, Thibaut Vazeille, Pierre Sonveauxwere analyzed using the Image J software (NIH). qRT-PCR Total RNA was isolated from cells using a Maxwell RSC instrument (Promega) following manufacturer’s instructions. qRT-PCR was performed as previously described64 with

(800) Role of muscle IL-6 in gender-specific metabolism in micePLOS ONEMarch 20, 2017Amalia Molinero, Antonio Fernandez-Perez, Aina Mogas, Mercedes Giralt, Gemma Comes, Olaya Fernandez-Gayol, Mario Vallejo, Juan Hidalgoleptin. Total RNA isolation from frozen hypothalamus was performed using Maxwell RSC simplyRNA tissue Kit and Maxwell RSC instrument (Promega), according to the manufacturer’s instructions. Reverse-transcription was performed using the

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