Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(741) An NMR-based approach reveals the core structure of the functional domain of SINEUP lncRNAsNucleic acids researchSeptember 18, 2020Ohyama T, Takahashi H, Sharma H, Yamazaki T, Gustincich S, Ishii Y, Carninci P.RT-PCR Total RNA was extracted by using a Maxwell RSC simplyRNA Cells Kit (Promega, Fitchburg, WI, USA) and Maxwell RSC Instrument in accordance with the manufacturer's protocol. Total RNA (500 ng) was reverse transcribed to cDNA by

(742) Analysis of major otosclerosis-associated variants in RELN and TGFB1 genes in Polish patientsArchives of Medical ScienceSeptember 16, 2020Dominika OziÄ™bÅ‚o, Sara DomagaÅ‚a, Marcin L. Leja, Henryk SkarÅ¼yÅ„ski, Monika OÅ‚dakfrom whole blood samples or buccal swabs using a standard salting out procedure or automated method (Maxwell RSC Instrument, Promega, Germany), respectively. Three SNPs located in the RELN gene (rs39335, rs39350, rs39374) and

(743) Isolation and Characterization of Two Novel Colorectal Cancer Cell Lines, Containing a Subpopulation with Potential Stem-Like Properties: Treatment Options by MYC/NMYC InhibitionCancersSeptember 10, 2020Schulte Am Esch JSA, Windmöller BA, Hanewinkel J, Storm J, Förster C, Wilkens L, Krüger M, Kaltschmidt B, Kaltschmidt C.mutation analysis was performed using automatically DNA-extraction with Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC instrument (Promega, Walldorf, Germany) according to the manufacturers guidelines in the Institute of Pathology

(744) LAMB3 Missense Variant in Australian Shepherd Dogs with Junctional Epidermolysis BullosaGenes (Basel)September 7, 2020Sarah Kiener, Aurore Laprais, Elizabeth A. Mauldin, Vidhya Jagannathan, Thierry Olivry, Tosso Leebfrom formalin-fixed paraffin-embedded (FFPE) tissue samples with the Maxwell RSC DNA FFPE Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). 2.3. Histopathological Examinations Skin punch biopsies (8 mm) were

(745) Dietary α-Linolenic Acid Counters Cardioprotective Dysfunction in Diabetic Mice: Unconventional PUFA ProtectionNutrientsSeptember 2, 2020Russell JS, Griffith TA, Naghipour S, Vider J, Du Toit EF, Patel HH, Peart JN, Headrick JP.using the Maxwell® RSC simplyRNA Tissue Kit (Promega Corporation, Alexandria, NSW, Australia) on the Maxwell® RSC instrument (Promega Corporation, Alexandria, NSW, Australia), per manufacturer’s instructions. RNA was eluted into

(746) COLORECTAL ADENOCARCINOMAS HARBORING ALK FUSION GENES: A CLINICOPATHOLOGIC AND MOLECULAR GENETIC STUDY OF 12 CASES AND REVIEW OF THE LITERATUREAm J Surg PatholSeptember 1, 2020Jerzy Lasota, Małgorzata Chłopek, Bartosz Wasąg, Artur Kowalik, Jason Christiansen, Jennifer Lamoureux, Alina Kuźniacka, Anna Felisiak-Gołąbek, Yalan Liu, Tiffany Ashley R. Reyes, et al.next-generation sequencing were previously reported.24 Briefly, nucleic acids were recovered from FFPE tumor samples using a Maxwell® RSC instrument (Promega, Madison, WI). Target-specific RNA libraries for next-generation sequencing (NGS) were constructed

(747) Recombinant thrombomodulin protects against LPS-induced acute respiratory distress syndrome via preservation of pulmonary endothelial glycocalyxBritish journal of pharmacologySeptember 1, 2020Suzuki K, Okada H, Takemura G, Takada C, Tomita H, Yano H, Muraki I, Zaikokuji R, Kuroda A, Fukuda H, et al.= 3 each). Total RNA was extracted using a simplyRNA Tissue Kit (Promega, Fitchburg, WI, USA) on a Maxwell RSC instrument. Gene expression analysis of the RNA samples was performed using an Agilent Expression Array (SurePrint

(748) Diagnosis of COVID-19 Based on Symptomatic Analysis of Hospital Healthcare Workers in Belgium: Observational Study in a Large Belgian Tertiary Care Center During Early COVID-19 OutbreakJ Occup Environ MedAugust 26, 2020Nele Van Loon, Mathieu Verbrugghe, Reinoud Cartuyvels, Dirk Ramaekersto each sample. Nucleic acid extraction was done with the Maxwell RSC Viral TNA kit (Promega) on the Maxwell RSC Instrument (Promega) according to the manufacturers’ instructions. An in-house rRT-PCR, based on the CDC oligonucleotide

(749) A New ERAP2/Iso3 Isoform Expression Is Triggered by Different Microbial Stimuli in Human Cells. Could It Play a Role in the Modulation of SARS-CoV-2 Infection?CellsAugust 24, 2020Saulle I, Vanetti C, Goglia S, Vicentini C, Tombetti E, Garziano M, Clerici M, Biasin M.Vacutainer tubes containing EDTA (BD Vacutainer, San Diego, CA, USA). Total DNA was extracted by DNA purification Maxwell® RSC Instrument (Promega, Fitchburg, WI, USA) and quantified using the Nanodrop 2000 Instrument (Thermo Scientific,

(750) Distinct Mutation Patterns Reveal Melanoma Subtypes and Influence Immunotherapy Response in Advanced Melanoma PatientsCancers (Basel)August 20, 2020Franz J. Hilke, Tobias Sinnberg, Axel Gschwind, Heike Niessner, German Demidov, Teresa Amaral, Stephan Ossowski, Irina Bonzheim, Martin RÃ¶cken, Olaf Riess, et al.was extracted from macrodissected 5µm paraffin sections using the Maxwell® RSC DNA FFPE Kit and the Maxwell® RSC Instrument (Promega, Madison, WI, USA) according to the manufacturer’s instructions. 2.2. Sequencing The sequencing

(751) Highly sensitive detection of SARS-CoV-2 RNA by multiplex rRT-PCR for molecular diagnosis of COVID-19 by clinical laboratoriesClinica Chimica ActaAugust 1, 2020Takayuki Ishige, Shota Murata, Toshibumi Taniguchi, Akiko Miyabe, Kouichi Kitamura, Kenji Kawasaki, Motoi Nishimura, Hidetoshi Igari, Kazuyuki Matsushitaextracted using a Maxwell RSC Viral Total Nucleic Acid Purification Kit (Promega, Madison, WI, USA) using a Maxwell RSC Instrument (Promega). Total nucleic acid was eluted in 50 μL of nuclease-free water. 2.4 Multiplex rRT-PCR A

(752) Draft Genome Sequences of Non-H 2 S-Producing Strains of Salmonella enterica Serovars Infantis, Enteritidis, Berta, and Kiambu in JapanMicrobiology Resource AnnouncementsJuly 23, 2020Hiroshi Asakura, Junko Sakata, Shiori Yamamoto, Shizunobu Igimiadded to the deep-well processing plate supplied in the kit, and DNA extraction was performed in a Maxwell RSC instrument (Promega) with default settings. The concentration and purity of the isolated DNA were checked with

(753) Comparison of Abbott ID Now, DiaSorin Simplexa, and CDC FDA Emergency Use Authorization Methods for the Detection of SARS-CoV-2 from Nasopharyngeal and Nasal Swabs from Individuals Diagnosed with COVID-19Journal of Clinical MicrobiologyJuly 23, 2020Daniel D. Rhoads, Sree S. Cherian, Katharine Roman, Lisa M. Stempak, Christine L. Schmotzer, Navid Sadriusing 7500 Fast instead of 7500 Fast Dx instrument, (ii) using an alternate RNA extraction method (Maxwell RSC instrument with viral TNA kit [catalog no. AS1330; Promega, Madison, WI, USA]), and (iii) interpreting “inconclusive”

(754) Draft Genome Sequences of Non-H2S-Producing Strains of Salmonella enterica Serovars Infantis, Enteritidis, Berta, and Kiambu in JapanMicrobiol Resour AnnouncJuly 23, 2020Hiroshi Asakura, Junko Sakata, Shiori Yamamoto, Shizunobu Igimiadded to the deep-well processing plate supplied in the kit, and DNA extraction was performed in a Maxwell RSC instrument (Promega) with default settings. The concentration and purity of the isolated DNA were checked with

(755) Expression of NANOG and Its Regulation in Oral Squamous Cell CarcinomaBioMed Research InternationalJuly 18, 2020Gašper Grubelnik, Emanuela Boštjančič, Aleš Grošelj, Nina ZidarMaxwell® RSC Tissue DNA Kit (Promega Corporation, Madison, USA). Automatic isolation was performed using Maxwell® RSC Instrument (Promega Corporation, Madison, USA), according to the manufacturer's instructions. All the reagents

(756) Mitochondrial PCK2 Missense Variant in Shetland Sheepdogs with Paroxysmal Exercise-Induced Dyskinesia (PED)Genes (Basel)July 9, 2020Jasmin Nessler, Petra Hug, Paul J. J. Mandigers, Peter A. J. Leegwater, Vidhya Jagannathan, Anibh M. Das, Marco Rosati, Kaspar Matiasek, Adrian C. Sewell, Marion Kornberg, et al.from the EDTA blood of affected dogs and healthy controls with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Illumina TruSeq PCR-free DNA libraries with 350 bp insert size of

(757) Cancer of Unknown Primary ( CUP ): genetic evidence for a novel nosological entity? A case reportEMBO Molecular MedicineJuly 7, 2020Silvia Benvenuti et al.three different regions of each metastases retrieved at warm autopsy and stored in RNAlater solution by Maxwell® RSC Instrument (Promega) using Maxwell® RSC miRNA Tissue Kit (Promega). Quantification was performed on a Bioanalyzer

(758) Microbiota in Waterlogged Archaeological Wood: Use of Next-Generation Sequencing to Evaluate the Risk of BiodegradationApplied SciencesJuly 4, 2020Federica Antonelli, Alfonso Esposito, Giulia Galotta, Barbara Davidde Petriaggi, Silvano Piazza, Manuela Romagnoli, Francesca Guerrierisamples were processed by the two different kits and total genomic microbial DNA was extracted with Maxwell®RSC Instrument (Promega Corporation, Madison, WI, USA) Appl. Sci. 2020, 10, 4636 4 of 21 Table 2. List of samples

(759) Leukocyte Telomere Length and Its Interaction with Germline Variation in Telomere-Related Genes in Relation to Pancreatic Adenocarcinoma RiskCancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive OncologyJuly 1, 2020Antwi SO, Bamlet WR, Rabe KG, Cawthon RM, Umudi I, Druliner BR, Sicotte H, Oberg AL, Jatoi A, Boardman LA, et al.buffy coat leukocytes stored at −80°C until DNA extraction. DNA extraction was performed using the Maxwell® RSC Instrument with appropriate kits (Promega) and quantitated using a Qubit Fluorometer. Details of the methods used

(760) Seroprevalence and B1 gene Phylogeny of Toxoplasma gondii of Dogs and Cats in Republic of KoreaThe Korean Journal of ParasitologyJune 26, 2020Yeojin Park, Jinhyeong Noh, Hyun-Ji Seo, Keun-Ho Kim, Subin Min, Mi-Sun Yoo, Bo-Ram Yun, Jong-Ho Kim, Eun-Jin Choi, Doo-Sung Cheon, et al.of Maxwell® RSC Whole Blood and Viral TNA Kit (Promega, Madison, Wisconsin, USA), respectively, and Maxwell® RSC Instrument (Promega). The cartridges to be used were placed in the deck tray(s). Each cartridge in the deck tray(s)

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