Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(741) Activity and safety of temozolomide in advanced adrenocortical carcinoma patientsEuropean Journal of EndocrinologyDecember 1, 2019Deborah Cosentini, Giuseppe Badalamenti, Salvatore Grisanti, Vittoria Basile, Ida Rapa, Sara Cerri, Andrea Spallanzani, Paola Perotti, Emanuela Musso, Marta Laganà, et al.after manual microdissection, for neoplastic cell enrichment (at least 50% of tumor cells), using the Maxwell® RSC instrument and tissue DNA Kit (Promega s.r.l). A total of 500 ng of gDNA was modified by bisulfite conversion using

(742) Enrichment technique to allow early detection and monitor emergence of KRAS mutation in response to treatmentScientific ReportsDecember 1, 2019Yoshiyasu Kitagawa et al.further. ctDNA was extracted from 500 μL of serum using the Maxwell RSC ccfDNA Plasma Kit and an automated Maxwell RSC Instrument (Promega, Madison, Wisconsin, USA) according to the manufacturer’s protocol, as described previously44.

(743) Isolation of photoprotective signal transduction mutants by systematic bioluminescence screening in Chlamydomonas reinhardtiiScientific ReportsDecember 1, 2019Ryutaro Tokutsu, Konomi Fujimura-Kamada, Tomohito Yamasaki, Takuya Matsuo, Jun Minagawaactivity as described above. RT-PCR analysis Total RNA from light-treated cells was extracted with the Maxwell RSC instrument (Promega) equipped with the Maxwell RSC simplyRNA Tissue Kit (Promega). The isolated RNA was quantified

(744) Optimisation of robust singleplex and multiplex droplet digital PCR assays for high confidence mutation detection in circulating tumour DNAScientific ReportsDecember 1, 2019Vicky Rowlands, Andrzej J. Rutkowski, Elena Meuser, T. Hedley Carr, Elizabeth A. Harrington, J. Carl BarrettEDTA blood collection tubes were thawed on wet ice. cfDNA was isolated from up to 2 ml plasma using a Maxwell® RSC instrument with ccfDNA Plasma Kit (AS1480) from Promega (Madison, Wisconsin, USA), KingFisher Flex System from

(745) Plasma Levels of snoRNAs are Associated with Platelet Activation in Patients with Peripheral Artery DiseaseInternational Journal of Molecular SciencesNovember 27, 2019Anne Yaël Nossent, Neda Ektefaie, Johann Wojta, Beate Eichelberger, Christoph Kopp, Simon Panzer, Thomas Gremmelisolated from 200 µL citrate-anticoagulated plasma, using the Maxwell® RSC simplyRNA Tissue Kit and the Maxwell® RSC instrument (Promega GmbH, Mannheim, Germany), according to the manufacturer’s protocol. A DNase treatment was included

(746) Changes of Metabolic Phenotype of Cardiac Progenitor Cells During Differentiation: Neutral Effect of Stimulation of AMP-Activated Protein KinaseStem cells and developmentNovember 15, 2019André E, De Pauw A, Verdoy R, Brusa D, Bouzin C, Timmermans A, Bertrand L, Balligand JL.isolated from cells using Maxwell RSC simplyRNA Tissue Kit (Promega, Madison, WI; AS1340) with a Maxwell RSC instrument (Promega). RNA was quantified using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific). Reverse

(747) The Lack of Mitochondrial Thioredoxin TRXo1 Affects In Vivo Alternative Oxidase Activity and Carbon Metabolism under Different Light ConditionsPlant and Cell PhysiologyNovember 1, 2019Igor Florez-Sarasa, Toshihiro Obata, NÃ¯Â¿Â½stor FernÃ¯Â¿Â½ndez Del-Saz, Jean-Philippe Reichheld, Etienne H Meyer, Manuel Rodriguez-Concepcion, Miquel Ribas-Carbo, Alisdair R Fernieisolated from lyophilized leaves by using Maxwell® RSC Plant RNA Kit 508 (Promega) and automated system Maxwell® RSC Instrument (Promega) according to the 509 manufacturer’s 510 spectrophotometer (Thermo Scientific, http://www.nanodrop.com/).

(748) Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tensionNature communicationsOctober 31, 2019Biancospino M, Buel GR, Niño CA, Maspero E, Scotto di Perrotolo R, Raimondi A, Redlingshöfer L, Weber J, Brodsky FM, Walters KJ, et al.lines or tissues was assessed by PCR. Messenger RNA from cultured cells or cysts was isolated with Maxwell RSC instrument (Promega) according to the manufacturer’s protocols. RNA from mouse kidney and intestine tissues was

(749)

Hyperprogressive Disease In Cervical Small Cell Carcinoma Treated By Immune Checkpoint Inhibitor

OncoTargets and TherapyOctober 30, 2019Zihan Xu, Liying Chen, Linpeng Zheng, Qiao Yang, Mingjing Chen, Jianmin Wang, Guangkuo Zhu, Zhengtang Chen, Jianguo Sun1D–I). For further analysis, tumor tissue and blood of the patient were used for extracting DNA by Maxwell® RSC Instrument according to the manufacturer’s instruction of the Maxwell RSC Tissue DNA Kit and Maxwell RSC Blood DNA Kit

(750) A TAC3 Missense Variant in a Domestic Shorthair Cat with Testicular Hypoplasia and Persistent Primary DentitionGenes (Basel)October 14, 2019Petra Hug, Patricia Kern, Vidhya Jagannathan, Tosso LeebDNA Extraction Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega). Additionally we used DNA from EDTA blood of 171 non-affected control cats of various breeds

(751) c-Myc shuttled by tumour-derived extracellular vesicles promotes lung bronchial cell proliferation through miR-19b and miR-92aCell death & diseaseOctober 7, 2019Borzi C, Calzolari L, Ferretti AM, Caleca L, Pastorino U, Sozzi G, Fortunato O.(dPCR) (Thermo Fisher Scientific) as previously described56. Briefly, total RNA was extracted using the Maxwell RSC Instrument (Promega, Madison, WI, USA) and Maxwell RSC miRNA Tissue Kit (Promega) following the manufacturer’s

(752) Towards Understanding Non-Infectious Growth-Rate Retardation in Growing PigsProteomesSeptember 11, 2019Ana M. Gutiérrez, Juan Sotillo, Sarah Schlosser, Karin Hummel, Ingrid Millercommercial extraction kit in an automated DNA extraction instrument (Maxwell® 16 Blood DNA Purification Kit&Maxwell® RSC Instrument for 16 samples, Promega Corporation, Madison, WI, USA, respectively). The extracted DNA samples were

(753) Liquid biopsy in lymphomas: a potential tool for refining diagnosis and disease monitoringJournal of Cancer Metastasis and TreatmentSeptember 4, 2019Riccardo Moia, Chiara Favini, Silvia Rasi, Clara Deambrogi, Valentina Ferri, Mattia Schipani, Sruthi Sagiraju, Abdurraouf Mokhtar Mahmoud, Ahad Ahmed Kodipad, Ramesh Adhinaveni, et al.that bind the plasma cfDNA[11]. The second approach relies on an automatic method that utilizes the Maxwell RSC Instrument coupled with Maxwell RSC ccfDNA Plasma Kit (Promega). This method consists in an automated nucleic acid purification

(754) The transcribed pseudogene RPSAP52 enhances the oncofetal HMGA2-IGF2BP2-RAS axis through LIN28B-dependent and independent let-7 inhibitionNature communicationsSeptember 4, 2019Oliveira-Mateos C, Sánchez-Castillo A, Soler M, Obiols-Guardia A, Piñeyro D, Boque-Sastre R, Calleja-Cervantes ME, Castro de Moura M, Martínez-Cardús A, Rubio T, et al.autoradiography film. RNA isolation and RT-qPCR analysis Total RNA, including miRNAs, was extracted using the Maxwell® RSC instrument with the Maxwell® RSC miRNA Tissue kit (Promega) according to the manufacturer’s recommendations. For

(755) Widespread FUS mislocalization is a molecular hallmark of amyotrophic lateral sclerosisBrain : a journal of neurologySeptember 1, 2019Tyzack GE, Luisier R, Taha DM, Neeves J, Modic M, Mitchell JS, Meyer I, Greensmith L, Newcombe J, Ule J, et al.fractions using the Promega Maxwell® RSC simplyRNA cells kit including DNase treatment, alongside the Maxwell® RSC instrument. Reverse transcription was performed using the RevertAid™ First Strand cDNA Synthesis Kit (Thermo Fisher Scientific

(756) Conversion of depolymerized sugars and aromatics from engineered feedstocks by two oleaginous red yeastsBioresource TechnologyAugust 1, 2019Alberto Rodriguez, Nadine Ersig, Gina M. Geiselman, Katharina Seibel, Blake A. Simmons, Jon K. Magnuson, Aymerick Eudes, John M. Gladdenby 143 qPCR using genomic DNA from wild-type and recombinant strains as template, extracted with a 144 Maxwell RSC Instrument and Maxwell RSC Plant DNA Kit (Promega, USA) from overnight 145 cultures in YPD broth. Templates were

(757) AKNA Frameshift Variant in Three Dogs with Recurrent Inflammatory Pulmonary DiseaseGenes (Basel)July 26, 2019Petra Hug, Linda Anderegg, Alexandra Kehl, Vidhya Jagannathan, Tosso Leeband Genotyping Genomic DNA was isolated from EDTA blood with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Ten Rough Collies consisting of 3 cases and 7 control dogs from an

(758) Endoplasmic Reticulum Associated Aminopeptidase 2 (ERAP2) Is Released in the Secretome of Activated MDMs and Reduces in vitro HIV-1 InfectionFrontiers in ImmunologyJuly 16, 2019Irma Saulle et al.accordance with the Declaration of Helsinki. Genotyping Analyses Total DNA extracted by DNA purification Maxwell® RSC Instrument (Promega) and quantified by the Nanodrop 2000 Instrument (Thermo Scientific) was used as a template

(759) Primary malignant melanoma of esophagus: clinicopathologic characterization of 20 cases including molecular genetic profiling of 15 tumorsModern PathologyJuly 1, 2019Jerzy Lasota, Artur Kowalik, Anna Felisiak-Golabek, Sebastian ZiÄ™ba, Piotr Waloszczyk, Marek Masiuk, Jaroslaw Wejman, Justyna Szumilo, Markku Miettinensections were available. DNA was extracted from 5 to 10 5 μ sections using Maxwell® RSC DNA FFPE kit and Maxwell® RSC instrument (Promega, Madison, WI) following manufacturer’s protocol provided at www.promega.com. All 15 tumors

(760) Intracellular Zn²⁺ transients modulate global gene expression in dissociated rat hippocampal neuronsScientific reportsJune 28, 2019Sanford L, Carpenter MC, Palmer AE.unless specified otherwise. RNA extraction for next-generation sequencing was performed using a Promega Maxwell RSC Instrument. Quantitative PCR was performed using a BIO-RAD CFX384 Real Time PCR Detection System instrument. In vitro

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