Maxwell RSC Instrument from Promega

Product Specs
ItemMaxwell RSC Instrument
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberAS4500
QuantityEA
Processing Time30 to 60 min (depending on sample type and method)
CapacityUp to 16
Dimensions(WxDxH) 330.2 x 345.2 x 299.7 mm (13 x 13.6 x 11.8 in)
UV LampAvg. lifetime approximately 6,000 hrs
Molecular Weight~85kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(761) Sequential Changes in the Host Gut Microbiota During Infection With the Intestinal Parasitic Nematode Strongyloides venezuelensisFrontiers in Cellular and Infection MicrobiologyJune 25, 2019Tanzila Afrin, Kazunori Murase, Asuka Kounosu, Vicky L. Hunt, Mark Bligh, Yasunobu Maeda, Akina Hino, Haruhiko Maruyama, Isheng J. Tsai, Taisei Kikuchitransferred to the Maxwell RSC Cartridge (Promega). Automatic DNA extraction was then performed with the Maxwell RSC instrument (Promega). The yield and purity of the extracted DNA were evaluated using NanoDrop (Thermo Fisher Scientific)

(762) A SIX6 Nonsense Variant in Golden Retrievers with Congenital Eye MalformationsGenes (Basel)June 14, 2019Petra Hug, Linda Anderegg, Nicole DÃ¼rig, Vincent Lepori, Vidhya Jagannathan, Bernhard Spiess, Marianne Richter, Tosso Leebblood of 30 members of an extended Golden Retriever family with the Maxwell RSC Whole Blood Kit using a Maxwell RSC instrument (Promega, Dübendorf, Switzerland). Additionally we used DNA from EDTA blood of 459 non-affected control

(763) Genome-Wide Association Studies Based on Equine Joint Angle Measurements Reveal New QTL Affecting the Conformation of HorsesGenes (Basel)May 14, 2019Annik Imogen Gmel, Thomas Druml, Rudolf von NiederhÃ¤usern, Tosso Leeb, Markus Neuditschkogenomic DNA was isolated from the EDTA blood samples using the Maxwell RSC Whole Blood DNA Kit and the Maxwell RSC Instrument (Promega). The Lipizzan sample regrouped photographs and DNA from 224 breeding animals (125 stallions

(764) Fusion of the Genes PHF1 and TFE3 in Malignant Chondroid SyringomaCancer Genomics - ProteomicsApril 9, 2019IOANNIS PANAGOPOULOS, LUDMILA GORUNOVA, MARIUS LUND-IVERSEN, ASSIA BASSAROVA, SVERRE HEIMaccording to the manufacturer’s instructions (BioRad, Oslo, Norway). Genomic DNA was extracted using the Maxwell RSC Instrument and the Maxwell RSC Tissue DNA Kit (Promega, Madison, WI, USA) and the concentration was measured using

(765) Circulating Cell-Free mtDNA Contributes to AIM2 Inflammasome-Mediated Chronic Inflammation in Patients with Type 2 DiabetesCellsApril 8, 2019Bae JH, Jo SI, Kim SJ, Lee JM, Jeong JH, Kang JS, Cho NJ, Kim SS, Lee EY, Moon JS.stored at −80 °C. The ccf-DNA was isolated from the plasma (100 μL) using a Maxwell® RSC Instrument (AS4500, Promega, Madison, WI, USA) and a Maxwell® RSC ccf-DNA Plasma Kit (AS1480, Promega), according to the

(766) Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cellsBMC cancerMarch 21, 2019Bakke J, Wright WC, Zamora AE, Oladimeji P, Crawford JC, Brewer CT, Autry RJ, Evans WE, Thomas PG, Chen T.quantitative reverse transcription PCR RNA was extracted with a Maxwell RSC simplyRNA Tissue Kit and a Maxwell RSC Instrument (Promega, Madison, WI). The RNA concentration was measured with a NanoDrop 8000 UV-Vis Spectrophotometer

(767) 2-Methacryloyloxyethyl phosphorylcholine (MPC)-polymer suppresses an increase of oral bacteria: a single-blind, crossover clinical trialClinical Oral InvestigationsFebruary 8, 2019Natsumi Fujiwara, Hiromichi Yumoto, Koji Miyamoto, Katsuhiko Hirota, Hiromi Nakae, Saya Tanaka, Keiji Murakami, Yasusei Kudo, Kazumi Ozaki, Yoichiro MiyakeMaxwell® Rapid Sample Concentrator (RSC) DNA FFPE Kit-PKK Custom (Promega Corporation, Wisconsin, USA) and Maxwell® RSC Instrument (Promega Corporation) according to the manufacturer’s instructions. In this study, we did not exclude

(768) Comprehensive mutation profiling and mRNA expression analysis in atypical chronic myeloid leukemia in comparison with chronic myelomonocytic leukemiaCancer MedicineFebruary 1, 2019Muhammad Faisal, Helge Stark, Guntram BÃ¼sche, Jerome Schlue, Kristin Teiken, Hans H. Kreipe, Ulrich Lehmann, Stephan Bartelspatients under study. 2.1 Nucleic acid extraction Extraction of DNA and RNA was performed with the Maxwell RSC instrument (Promega, Madison, WI, USA) according to the manufacturer's recommendations. Three to five sections

(769) Preventive Effect of Spontaneous Physical Activity on the Gut-Adipose Tissue in a Mouse Model That Mimics Crohn's Disease SusceptibilityCellsJanuary 9, 2019Maillard F, Vazeille E, Sauvanet P, Sirvent P, Bonnet R, Combaret L, Chausse P, Chevarin C, Otero YF, Delcros G, et al.again to ensure that all beads were removed. Then, supernatants were loaded in the cartridges of a Maxwell® RSC Instrument (Promega, Madison, WI, USA) for DNA extraction. DNA concentration was determined with a Qubit Fluorometer

(770) High intensity interval training promotes total and visceral fat mass loss in obese Zucker rats without modulating gut microbiotaPloS oneJanuary 1, 2019Maillard F, Vazeille E, Sauvanet P, Sirvent P, Combaret L, Sourdrille A, Chavanelle V, Bonnet R, Otero YF, Delcros G, et al.centrifuged again to ensure that all beads were removed. The supernatants were then placed in cartridges in Maxwell RSC Instrument (Promega) to extract DNA. DNA concentration was determined by Qubit Fluorometric Quantitation (Invitrogen)

(771) BRCA2 controls DNA:RNA hybrid level at DSBs by mediating RNase H2 recruitmentNature CommunicationsDecember 18, 2018Giuseppina D’Alessandro et al.extraction Total RNA from cultured cells was extracted with Maxwell® RSC simplyRNA Tissue Kit with the Maxwell® RSC Instrument (Promega), according to manufacturer instructions. For dilncRNAs detection, chromatin-bound RNA was

(772) High-sensitivity assay for monitoring ESR1 mutations in circulating cell-free DNA of breast cancer patients receiving endocrine therapyScientific ReportsDecember 1, 2018Laura Lupini, Anna Moretti, Cristian Bassi, Alessio Schirone, Massimo Pedriali, Patrizia Querzoli, Roberta Roncarati, Antonio Frassoldati, Massimo Negrinicentrifugation at 1,000 g for 10 min and stored at −80 °C. DNA was extracted from 1 mL of plasma using the Maxwell RSC instrument (Promega) and Maxwell RSC ccfDNA plasma kit (Promega), according to the manufacturer’s instructions.

(773) Saliva samples as a source of DNA for high throughput genotyping: an acceptable and sufficient means in improvement of risk estimation throughout mammographic diagnosticsEuropean Journal of Medical ResearchDecember 1, 2018U. G. Poehls, C. C. Hack, A. B. Ekici, M. W. Beckmann, P. A. Fasching, M. Ruebner, H. Huebnerbuffer. For the automated magnetic bead-based DNA purification, the saliva samples were processed on the Maxwell RSC instrument (Promega, Germany) using the Maxwell RSC Blood DNA Kit. For preprocessing of the samples, 300 µl of

(774) Combination of PURE-DNA extraction and LAMP-DNA amplification methods for accurate malaria diagnosis on dried blood spotsMalaria JournalDecember 1, 2018Jeanne PerpÃ©tue Vincent, Kanako Komaki-Yasuda, Moritoshi Iwagami, Satoru Kawai, Shigeyuki Kanousing a Q IAamp® DNA Mini Kit (QIAGEN, Hilden, Germany). An automated DNA extraction system, the Maxwell RSC Instrument (Promega, Madison, WI, USA), was also used. With this instrument, DNA samples were extracted from 200

(775) Long Non-Coding RNA Modulation of VEGF-A during HypoxiaNon-Coding RNANovember 20, 2018Tiina Nieminen, Tristan A. Scott, Feng-Mao Lin, Zhen Chen, Seppo Yla-Herttuala, Kevin V. MorrisQuantitative Polymerase Chain Reaction RNA was extracted with Maxwell® RSC simplyRNA Cells Kit (Promega) and Maxwell® RSC instrument (Promega) or with TRIzol Reagent (Thermo Fisher Scientific) according to manufacturer’s instructions.

(776) miR-182 and miR-183 Promote Cell Proliferation and Invasion by Targeting FOXO1 in MesotheliomaFrontiers in OncologyOctober 22, 2018Rui Suzuki, Vishwa Jeet Amatya, Kei Kushitani, Yuichiro Kai, Takahiro Kambara, Yukio Takeshimathe microRNA inhibitors and siRNA transfected using a Maxwell RSC simplyRNA Cells kit (Promega) on a Maxwell® RSC Instrument (Promega) according to the manufacturer's protocol. A total of 200 ng of RNA was reverse-transcribed

(777) MicroRNA-199a-3p and MicroRNA-199a-5p Take Part to a Redundant Network of Regulation of the NOS (NO Synthase)/NO Pathway in the EndotheliumArteriosclerosis, Thrombosis, and Vascular BiologyOctober 1, 2018Virginie Joris, Elvira Leon Gomez, Lisa Menchi, Irina Lobysheva, Vittoria Di Mauro, Hrag Esfahani, Gianluigi Condorelli, Jean-Luc Balligand, Daniele Catalucci, Chantal DessyDNAse 1, excepted for samples where miR inhibition by LNA was measured. Samples were processed using Maxwell RSC Instrument (Promega) for miRNA extraction and eluted in nuclease-free water. miRNA Reverse Transcription and Quantitative PCR

(778) Parkin and PINK1 mitigate STING-induced inflammationNatureSeptember 13, 2018Danielle A. Sliter et al.and nuclear DNA was isolated from serum using the Maxwell RSC ccfDNA Plasma Kit (Promega) using the Maxwell RSC Instrument (Promega), then quantified using digital droplet PCR on a Maxwell as described in Ye, W. et. al.34.

(779) IMiDs mobilize acute myeloid leukemia blasts to peripheral blood through downregulation of CXCR4 but fail to potentiate AraC/Idarubicin activity in preclinical models of non del5q/5q- AMLOncoImmunologySeptember 2, 2018Belen Lopez-Millan et al.gene expression profiling (GEP) as described.29 Total RNA was then extracted using a Maxwell® RSC simplyRNA Cells Kit in a Maxwell® RSC Instrument (Promega) and its quality checked in the Agilent 2100 Bioanalyzer. Total RNA samples were labeled with

(780) RNA based individualized drug selection in breast cancer patients without patient-matched normal tissueOncotargetAugust 17, 2018Michael Forster et al.Maxwell RSC RNA FFPE Kit (Cat # AS1440, Promega, Madison, WI, USA) on the Maxwell automated system (Cat # AS4500, Promega, Madison, WI, USA) according to the manufacturers' protocol. This RNA was quantitated using

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