Dual-Luciferase Reporter Assay System 10-Pack E1960 from Promega

Product Specs
ItemDual-Luciferase Reporter Assay System 10-Pack
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1960
ReactivityMammalian
Detection TargetLuciferase
SpeciesMammalian
Sample TypeMammalian cells
Quantity1,000 assays
Assay Time4 seconds
Sensitivityattomole ( < />^–18)
MethodLuciferase
Molecular Weight~70–76kDa

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutationsMolecular Therapy OncologyJune 18, 2026Joshua Sammons, Jianguo Chen, Kari Thrasher, Lianwu Fu, Ming Du, Hui Wen, J. Robert Bostwick, Paige Vinson, Omar Moukha-Chafiq, Corinne Augelli-Szafran, et al.readthrough compound ± enhancers. 24 h later, cells were lysed with passive lysis buffer (Promega, E1960). Firefly and Renilla activities were assayed using the Promega Dual-Luciferase Reporter Assay System

(2) 2-Hydroxyestradiol regulates extracellular matrix deposition through estrogen receptor beta activation in airway smooth muscle cellsMolecular and Cellular EndocrinologyMay 1, 2026Ashish Kumar, Mohammad Irshad Reza, Anurag Banerjee, Nilesh Sudhakar Ambhore, Premanand Balraj, Buddhadev Layek, Michael A. Thompson, John R. Hawse, Christina M. Pabelick, Y.S. Prakash, et al.acquired from Santa Cruz Biotechnology (Dallas, TX, USA). Dual-Luciferase Reporter Assay System 10-Pack (E1960) was obtained from Promega. IRDye goat anti-rabbit and goat anti-mouse secondary antibodies were used

(3) Role of miR-101a in targeting Cox-2 to attenuate chondrocyte hypertrophic differentiation and osteoarthritis progressionGenes & DiseasesMay 1, 2026Rui Mi, Jinnan Chen, Tianxiang Zhu, Huiqin Bian, Rong Wei, Rushuang Deng, Tiaotiao Han, Qian Wang, Yaojuan Lu, Longwei Qiao, et al.Firefly and Renilla luciferase were measured with a Dual-Glo luciferase reporter assay system (Promega, E1960, Wisconsin, USA). Induction of mouse knee OA model and intra-articular injection Animal experiments

(4) Targeting adipocyte ESRRA alleviates osteoarthritis via interrupting inter-organelle crosstalk of complement C3-CFD-MAC cascadeBone ResApril 29, 2026Tongling Huang, Zihui Wang, Lu Gao, Jun Gao, Zhaocheng Lu, Pengda Li, Chon Him Choy, Zhuolei Yuan, Yanting Zhong, Chang-An Geng, et al.lysed, and luciferase activity was measured with a dual-luciferase reporter assay system (Promega, E1960) and Microplate Luminometer (Promega Glo-Max 96, E6521). The final analysis was normalized to Renilla

(5) Repression of EEF1D by KSHV RTA promotes viral lytic reactivationJournal of VirologyApril 21, 2026Min Xiang, Lei Yu, Chunyan Han, Liang Huang, Lianghui Dong, Lei Bai, Shuwen Wu, Ke LanRenilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1960) according to the manufacturer’s instructions. To account for variations in transfection efficiency,

(6) Delactylation of viral proteins by SIRT1 suppresses influenza A virus replicationmBioApril 8, 2026Dongdong Chen, Gejie Zhao, Jia Zhou, Peng Sun, Shutong He, Changjie Lv, Yuhai Chen, Shouhai Zhu, Ming Gao, Guijie Guovector. The luciferase activity was measured using the dual-luciferase reporter assay system (catalog no. E1960, Promega, USA) and a Luminoskan Ascent luminometer (Thermo Scientific, USA) according to the manufacturer’s

(7) Fine-mapping reveals BcNAC153 act as a negative regulator controlling high temperature tolerance in Brassica rapaMol HorticApril 2, 2026Gaofeng Liu, Entong Li, Qian Hu, Yaliang Xu, Changwei Zhang, Ying Li, Zixin Zhang, Xilin Houcamera was used to observe luciferase activity after 48–96 h. The Dual-Luciferase Reporter Assay System E1960 (Promega, Madison, cat. #e1910) was used to measure the fluorescence intensity of luciferase and renilla

(8) SMALL GRAIN 5 encodes a heat shock transcription factor controlling grain size and plant architecture in riceFront Plant SciApril 1, 2026Chenjie Wang, Nannan Han, Zhao Li, Chen Zhou, Guansong Li, Yongqing Pan, Baolan Zhang, Ke Huang, Yunhai Li, Yingjie LiRenilla luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, E1960, Madison, WI, USA) according to the manufacturer’s instructions. Transcriptional activation activity

(9) The birth, death, and evolutionary compensation of uORFs in DrosophilaNucleic Acids ResMarch 26, 2026Mengze Xu, Chenlu Liu, Wanting Jin, Yuanqiang Sun, Yuange Duan, Xiaolu Tang, Jian Luluciferase enzyme and firefly luciferase enzyme were measured via the Dual-Luciferase Reporter Assay System (E1960, Promega) at 24 h after transfection. Moreover, RNA extraction from cells and reverse transcription

(10) CRM1-dependent nuclear export of TRIM28 promotes MAVS K48-linked ubiquitination and suppresses RIG-I-mediated antiviral responseFrontiers in ImmunologyMarch 24, 2026Ruihua Xin, Wenshu Zou, Zhengying Qiu, Lina Huang, Xiaohan Jing, Qilin Huang, Zixiang Zhu, Jianxi Li, Mutien-Marie Gariglianykits were also from Thermo Fisher Scientific/Invitrogen. The Dual-Luciferase® Reporter Assay System (E1960) was from Promega (USA). Fetal bovine serum (FBS, 10270), penicillin-streptomycin (15140-122), and GlutaMAX Supplement

(11) WNT7B drives a program for pancreatic cancer subtype switching and progressioniScienceMarch 20, 2026Joep Sprangers, Jeroen M. Bugter, Despina Xanthakis, Michiel Boekhout, Rutger N.U. Kok, Veerle E. Geurts, Hans Clevers, Lodewijk A.A. Brosens, Frederike Dijk, Maria J. Rodríguez Colman, et al.iScript cDNA Synthesis Kit,Bio-Rad,1708890 iQ SYBR Green Mix,Bio-Rad,1708880 Dual-Luciferase Reporter Assay System,Promega,E1960 ,, Deposited data,Deposited data,Deposited data ,, SPACIOUS-2 cohort RNA expression data,"J Annelie

(12) The Galectin-3-binding protein promotes angiogenesis in pancreatic cancer via simultaneous upregulation of VEGFA and direct HUVEC activation mediated by and VAMP5-STAT3Cell Communication and SignalingMarch 18, 2026Ye Jin Lim, Dong Woo Son, Eun Ji Lee, Chae Won Yu, Yun Ok Oh, Min Jung Cha, Hyori Kim, Kyunggon Kim, Suhwan Changcells expressing luciferase. Subsequently, we performed the Dual-Luciferase® Reporter (DLR™) Assay (E1960, Promega) to validate luciferase expression. Antibody treatment of the pancreatic cancer orthotopic

(13) The REDD1–NF-κB–miRNAs–eNOS/SIRT1 axis mediates obesity-induced endothelial cell senescence and hypertensionNature CommunicationsMarch 12, 2026Yoon Kyung Choi, Dong-Keon Lee, Minsik Park, Junyoung Byeon, Woo-Young Nam, Thuy Linh Lai, Kyu Nam Kim, Okhwa Kim, Sungwoo Ryoo, Jeong-Hyung Lee, et al.fresh medium for 24 h, and reporter gene activity was assessed using the Dual-Luciferase Assay Kit (#E1960, Promega, Madison, WI, USA). Firefly luciferase activity was normalized to Renilla luciferase activity. eNOS–NO–cGMP

(14) ROME, an ancient gene with a novel function in vertebrates, is a key modulator of embryonal development and cancer metastasisCancer Research CommunicationsMarch 1, 2026Anna Molotkova, Emre Deniz, Matthew Swift, Eric Glasgow, Junfeng Ma, Jeffrey A. Petro, Isabel Frye, Metin Ozdemirli, Dusan Pesic, Gerard P. Ahern, et al.and then luciferase assay was conducted with the Dual Luciferase Reporter Assay System Kit (Promega, #E1960) according to the manufacturer’s protocol. ROME expression across different cancer types The expression

(15) Activation of IRF3 in cardiomyocytes impairs mitochondrial oxidative function through PGC-1α inhibition and drives heart failureNature CommunicationsFebruary 27, 2026Manju Kumari, Ioannis Evangelakos, Anushka Deshpande, Kirstie A. De Jong, Nesrin Schmiedel, Nicolàs Palacio-Escat, Adriano de Britto Chaves-Filho, Roberto Carlos Frias-Soler, Glynis Klinke, Hyun Cheol Roh, et al.After 48 h of transfection, chemiluminescence was measured using a Dual-luciferase® Reporter Assay kit (E1960 Promega) on TECAN Spark multimode reader with injector module following manufacturer’s instructions.

(16) NRMLncR, a myocyte-enriched long non-coding RNA, enhances myogenesis in mousebioRxivFebruary 17, 2026Yufen Li, Yumei Zhou, Qing Ying Li, Justine Arrington, Mostafa F. Abdelhai, Yubo Wang, Junxiao Ren, Yung-Yu Cheng, Mingyi Xie, W. Andy Tao, et al.and luciferase activities were measured using the Dual-Luciferase Reporter Assay System (Promega, cat#E1960) following the manufacturer’s instructions. Firefly luciferase activity was normalized to Renilla luciferase

(17) Tumor-immune-neural circuit disrupts energy homeostasis in cancer cachexiaCancer CellFebruary 12, 2026Xiuhui Shi, Alex X. Arreola, Zhijun Zhou, Jingxuan Yang, Mingyang Liu, Yang Cai, Yu Ren, Hao Yuan, Qun Chen, Xinjie Chen, et al.Luciferase Reporter Assay Luciferase reporter assay was performed using the Dual Luciferase Reporter Assay System (#E1960; Promega) following the manufacturer’s protocol. Briefly, cells were seeded in a 24-well plate and co-transfected

(18) The transcription factor ANAC017 links mitochondrial retrograde signaling with the ubiquitin–proteasome system to control mitochondrial function in ArabidopsisPlant CommunicationsFebruary 9, 2026Yang Zhao, Michael Ogden, Ronghui Pan, Jianping Hu, Staffan Persson, Monika W. Murcha, Huixia Shou, Yan Wang, Ghazanfar Abbas Khan, James Whelanactivity was measured according to the instructions of the Dual-Luciferase Reporter Assay System (Promega, E1960). The Renilla reniformis luciferase (REN) gene driven by CaMV 35S was used as an internal control. Promoter

(19) Identification and characterization of a novel inhibitor of influenza A virus that acts by blocking nucleoprotein oligomerizationAntimicrobial Agents and ChemotherapyFebruary 4, 2026Vincent H. J. Leonard, Dianna B. Vidales, Benjamin R. Taft, Matthew J. Hesse, Patrick S. Lee, Mulugeta Mamo, Dirksen E. Bussiere, Karen C. Wolff, Kelli L. Kuhen, Laura Wedel, et al.Gene Assay System (Perkin-Elmer, cat #6066769) or Dual-Luciferase Reporter Assay System (Promega, cat #E1960), according to the manufacturer’s instructions. To determine cytotoxicity (CC50), cell viability was

(20) TIMELESS promotes LUAD growth via suppressing transferrin-mediated ferroptosis and reprograms the tumor microenvironment against anti-PD-1 immunotherapyCancer CommunicationsFebruary 3, 2026Chenchen Hu, Feiming Hu, Changjian Shao, Yuanli He, Liping Su, Daimei Shi, Lingying Yu, Yuanjie Sun, Jing Wang, Xiyang Zhang, et al.transcriptional activity of the TF promoter was assessed using a Dual Luciferase Reporter System (Promega, Cat. E1960). H1975 and A549 cells at 60% to 80% confluence were transfected with reporter plasmids carrying either

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(1)

7/29/2023Dual-Luciferase Reporter Assay System to Detect Firefly and Renilla Luciferase Activities Parijat SarkarThe Dual-Luciferase® Reporter Assay System has proven to be an invaluable tool in my research for measuring target gene transcription. By fusing a synthetic transcription factor with yeast Gal4 DNA-... Read Review
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2/2/2017Great Kit For Luciferase Assays Jake F.I’m studying regulatory sequences in embryonic stem cells. This kit was used to measure luciferase activity of my reporter after transfecting into mouse embryonic stem cells. I choose this kit because... Read Review
(1)

12/14/2016Luciferase Reporter Assay Arul ManiThe Dual-Luciferase Reporter (Promega) Assay System provides an efficient means of performing two reporter assays. In the assay, activities of firefly and Reilla luciferases are measured sequentially... Read Review
(1)

6/2/2016Luciferase Assay junyan taoThe activities of firefly and Renilla luciferases are measured sequentially from a single sample and the Renilla is used as the inter control to calculate the relativity changes on the interaction ... Read Review
(1)

1/7/2016Dual Luciferase Reporter, Promega Ganesh PusapatiPrimary cilia are tiny microtubule based structures that project from the cell surface. These structures serve as the signaling hubs for the Sonic hedgehog signaling. The goal of this experiment is ... Read Review
(1)

11/3/2015Lucifersae Assay Somasundaram RaghavanReporter systems are generally used to study eukaryotic gene expression. These dual reporters are generally used for experimental accuracy. Basically here we can express as well as measure the two ... Read Review

Dual-Luciferase Reporter Assay System From Promega
The luciferase assay is one of the most well-known and convenient reporter assays. In particular, ... Read More
Dual-Luciferase® Reporter Assay System from Promega
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Dual-Luciferase Reporter Assay System 10-Pack

Dual-Luciferase Reporter Assay System 10-Pack from Promega

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Promega

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Dual-Luciferase Reporter Assay System From Promega

Dual-Luciferase® Reporter Assay System from Promega

Dual-Luciferase Reporter Assay System 10-Pack from Promega

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