Zombie Green™ Fixable Viability Kit 423111 from BioLegend

Product Specs
ItemZombie Green™ Fixable Viability Kit
CompanyBioLegend
Price $89.00Supplier Page View Company Product Page
Catalog Number423111
Quantity100 tests
ApplicationsFC - Quality tested, ICFC, ICC - Verified

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Human receptive endometrial assembloid for deciphering the implantation windoweLifeApril 1, 2026Yu Zhang, Rusong Zhao, Chaoyan Yang, Jinzhu Song, Peishu Liu, Yan Li, Boyang Liu, Tao Li, Changjian Yin, Minghui Lu, et al.min at room temperature. Cells were then stained with Zombie Green Fixable Viability Kit (Biolegend 423111) in the dark for 20 min at room temperature. For staining of macrophages, 7×104 single cells were dealt

(2) Endothelial Nitric Oxide Synthase-Dependent Mechanism of Hydroxyurea-Induced S-Phase Arrest in Erythroid CellsAntioxidants (Basel)March 31, 2026Teodora Dragojević, Dragoslava Đikić, Slavko Mojsilović, Miloš Lazarević, Dejan Milenković, Olivera Mitrović Ajtić, Emilija Živković, Miloš Diklić, Tijana Subotički, Juan F. Santibanez, et al.After washing, propidium iodide (PI) (P1304MP, ThermoFisher Scientific) or ZombieGreen (BioLegend, 423111, San Diego, CA, USA) was added, and the samples were analyzed using a BD FACSCalibur flow cytometer.

(3) Liver-directed AAV-IL-10 therapy enhances CD8+ T cell-mediated immunity against hepatocellular carcinomaJ Immunother CancerMarch 27, 2026Chia-I Lin, Yi-Wei Chiu, Meng-Syuan Yen, Yu-Wen Wang, Ying-Hsuan Wu, Ya-Hui Chuang563061 Anti-mouse CD44 (clone IM7),BV786,BD,Cat# 563736 Zombie Green Fixable Viability Kit,FITC,BioLegend,Cat# 423112 Zombie UV Fixable Viability Kit,UV,BioLegend,Cat# 423107 Zombie Red Fixable Viability Kit,PE/Dazzle594,BioLegend,Cat#

(4) Effects of Platelet-Rich Plasma Dose and Application Strategy on Post-Thaw Spermatological Parameters in Goat SemenVet SciMarch 5, 2026Ahmet Eser, Kemal Bağcı, Abdurrahman Alakuş, Aslıhan Çakır Cihangiroğlu, İkra Karaağaç, Selin Yağcıoğlu, Ramazan Arıcı, Kamber DemirSperm Viability Cell viability was determined using the Zombie Green™ Fixable Viability Kit (Cat. no. 423112, BioLegend, San Diego, CA, USA) according to the manufacturer’s protocol with minor modifications [40].

(5) Citrate clearance is a major function of aconitase 2 in the canonical TCA cycleCellFebruary 27, 2026Abigail Xie, Julia S. Brunner, Sangita Chakraborty, Angela M. Montero, Anna E. Bridgeman, Katrina I. Paras, Ruobing Cui, Maider Fagoaga-Eugui, Monika Komza, Paige K. Arnold, et al.Zombie NIR™ Fixable Viability Kit (Biolegend, 423105) or Zombie Green™ Fixable Viability Kit (Biolegend, 423111), followed by fixation with 4% PFA in PBS and permeabilization with saponin-based permeabilization and

(6) Reversing T cell dysfunction in a novel in vitro model of T cell exhaustion reveals differential roles of RASA2Frontiers in ImmunologyFebruary 25, 2026Hilal Saraç, Rachael Nicholson, Rebecca N. Graham, Meera Augustus, Raha Taghavi, Dympna J. Connolly, Lindsay Lim, Sofia Lourencocondition were seeded and washed as above and stained with Zombie Green Fixable Viability Kit (BioLegend, #423111) at room temperature for 20 minutes protected from light. Cells were washed once in FACS buffer and

(7) Mast-cell derived nerve growth factor drives ILC2 pro-tumoral functions in bladder cancerNature CommunicationsFebruary 21, 2026Maryline Falquet, Hajar El Ahanidi, Alejandra Gomez-Cadena, Ziyang Su, Anthony Cornu, Tania Wyss, Burak Kizil, Robert Pick, Katayoun Falamaki, Pratyaksha Wirapati, et al.Lot B318208). Dead cells were excluded using the viability Zombie Green Dye (1:2000, Biolegend Cat 423112, Lot B314570). Bulk mRNAseq on TrkA+ and TrkA- ILC2s Total RNA was extracted using the RNEasy Micro

(8) Pharmacological perturbation of splicing elicits SMG1 reduction: Implications for cancer therapyiScienceFebruary 20, 2026Martin Barainka, Angelina Zheleva, Angela Pérez-Cervera, Helena Villanueva, Daniel Meraviglia-Crivelli, Beatriz Moreno, Fernando Pastor,Applied Biosystems,Cat#4385612 ONE-Glo™ Luciferase Assay System,Promega,Cat#E6120 Zombie Green™ Fixable Viability Kit,BioLegend,Cat#423111 ,, Deposited data,Deposited data,Deposited data ,, BRCA scRNAseq data,lambrechtslab,https://lambrechtslab.sites.vib.be/en/single-cell Melanoma

(9) A GDF-15–GFRAL axis controls autoimmune T cell responses during neuroinflammationNature ImmunologyJanuary 15, 2026Jana K. Sonner, Audrey Kahn, Lars Binkle-Ladisch, Jan Broder Engler, Beatrice Haack, Christina Zeiler, Lisa Unger, Simone Bauer, Felix Fischbach, Giovanni Almanzar, et al.analysis by staining with Zombie Aqua, Green, Yellow and NIR Fixable Viability Stains (BioLegend, 423101, 423112, 423104, 423106) or 0.8 µM Alexa Fluor 750 NHS (Invitrogen, A20011). For the determination of absolute

(10) Intranasal replicon vaccine establishes mucosal immunity and protects against H5N1 and H7N9 influenzaNature CommunicationsJanuary 13, 2026Matthew R. Ykema, Michael A. Davis, Darshan N. Kasal, Madeleine F. Jennewein, Ethan Lo, Jasneet Singh, Samuel Beaver, Noah Cross, Eduard Melief, Sierra Reed, et al.stimulation solution. Spleen and lung lymphocytes were stained for viability with Zombie Green (BioLegend #423112; 1:1000), then Fc receptors were blocked with CD16/CD32 antibody (Invitrogen #14-0161-82; 1:200). Cells

(11) A Brain Endothelial Cell Caveolin-1/CXCL10 Axis Promotes T Cell Transcellular Migration Across the Blood-Brain BarrierASN NeuroDecember 31, 2025Troy N. Trevino, Ali A. Almousawi, Remy Martins-Goncalves, Andrea Ochoa-Raya, KaReisha F. Robinson, Genesis L. Abad, Leon M. Tai, Suellen D. Oliveira, Richard D. Minshall, Sarah E. Lutzafter selection (Fig. S1). For this, we incubated with fixable viability dye (Zombie Green, Biolegend 423111, 1:200) and Fc receptor blockade (anti-mouse CD16/32, Biolegend 101319, 1:200) followed by incubation

(12) Deletion of Ptpn2 in B cells promotes autoimmunity via TLR and JAK/STAT signalingJCI InsightDecember 22, 2025Bridget N. Alexander, Soojin Kim, Kristen L. Wells, Maya J. Hunter, Kevin P. Toole, Scott M. Wemlinger, Daniel P. Regan, Andrew Getahun, Mia J. SmithCD25-BV421(BioLegend, 102043); and CD127-PeCy7 (BioLegend, 121120). Viability stains included Zombie Green (BioLegend, 423112), Zombie NIR (BioLegend, 423105), or L/D Fixable Blue (Invitrogen, L34961). Single-color controls were

(13) Preclinical synergistic effects when combining a radiolabelled HER2-targeting single domain antibody with PARP inhibitor OlaparibJournal of Translational MedicineDecember 13, 2025Jonatan Dewulf, Laurent Navarro, Nina Dumauthioz, Marion Berdal, Surasa Nagachinta, Vaiva Gaspariunaite, Ana Rita Pombo Antunes, Tony Lahoutte, Sam Massa, Nick Devoogdt, et al.were washed, collected, and stained with 1/1000 Zombie Green™ Fixable viability kit (Biolegend, cat 423112) diluted in PBS for 20 min at 4°C. Cells were washed twice with FACS buffer (PBS, 1% FBS, 2.5 mM EDTA)

(14) Enhanced Glioblastoma Immunotherapy via SMAC Mimetic Dose Escalation and TGFβ BlockadeNeuro-Oncology AdvancesDecember 10, 2025Kyle Malone, Melanie Dugas, Nathalie Earl, Tommy Alain, Robert G Korneluk, Eric LaCasse, Shawn T BeugZombie Violet Fixable Viability Kit,BV421 Equivalent,BioLegend,423114 Zombie Green Fixable Viability Kit,AF488/GFP Equivalent,BioLegend,423112 Anti-mouse phosphatidylserine,AF488,Sigma Aldrich,16-256 Anti-mouse calreticulin,PE,Novus,NBP1-47518PE Anti-mouse

(15) Lipid Nanoparticle Library Screen Reveals Lymphatic Endothelial Cell-Targeting Lipid Nanoparticle for Delivering Vascular Endothelial Growth Factor C mRNA after Lymphatic InjuryACS NanoDecember 9, 2025Eleftheria Michalaki, Kiyoung Jeong, Rachel Chin, Zhiming Qi, Lauren Liebman, Yarelis González-Vargas, Elisa Schrader Echeverri, Kalina Paunovska, Hiromi Muramatsu, Norbert Pardi, et al.quantification with the Zombie NIR Fixable Viability Kit following the manufacturer’s protocol (1:100 dilution, 423111; BioLegend, San Diego, CA). Subsequently, cells were washed with FACS buffer (10 mg/mL BSA (A7906; Sigma-Aldrich)

(16) Short-term animal product dietary restriction alters metabolic profiles and modulates immune functionCommunications MedicineDecember 3, 2025Eleni M. Loizidou, Aikaterini Palaiokrassa, Samuel Opoku Asiedu, Alexandros Simistiras, Petros Barmpounakis, Stavros Glentis, Maria Anezaki, Kristina V. Bergersen, Iosif Chatzimichalis, Eleni Kotsanopoulou, et al.min at room temperature. Cells were stained with Zombie GreenTM (Fixable Viability Kit, BioLegend, #423111) at a 1:100 dilution in 1x PBS and incubated for 30 min. Fc receptor blocking (anti-CD16/CD32) was performed

(17) PAX3-FOXO1 Drives Targetable Cell State–Dependent Metabolic Vulnerabilities in RhabdomyosarcomaCancer ResearchDecember 1, 2025Katrina I. Paras, Julia S. Brunner, Angela M. Montero, Jacob A. Boyer, Benjamin T. Jackson, Sangita Chakraborty, Abigail Xie, Kristina Guillan, Armaan Siddiquee, Lourdes Pajuelo Pajuelo Torres, et al.Cells labeled with EdU were collected and stained with ZombieGreen Fixable Viability dye (Biolegend, 423111), followed by fixation with 4% paraformaldehyde and permeabilization with saponin-based permeabilization

(18) 3-in-one PD-1CAR Tregs: A bioengineered cellular therapy for target engagement, activation, and immunosuppression with reparative potentialiScienceNovember 21, 2025Shuyun Dong, Tianxiao Zhang, Yujia Zhai, Lauren C. Naatz, Noel G. Carlson, John W. Rose, Brian Evavold, Mingnan ChenDynabeads™ Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation,Thermo Fisher,Cat#11456D Zombie Green™ Fixable Viability Kit,Biolegend,Cat#423111 Zombie Violet™ Fixable Viability Kit,Biolegend,Cat#423113 DAPI,invitrogen,Cat#D21490 PE Annexin V,Biolegend,Cat#640908 Cell

(19) Eradicating Drug-tolerant Persister Cells in EGFR- Mutated Non–Small Cell Lung Cancer by Targeting TROP2 with CAR-T Cellular TherapyCancer DiscoveryNovember 3, 2025Simon Baldacci, Elliott J. Brea, Francesco Facchinetti, Zhaorong Li, Kenneth Ngo, Soumya Malhotra, Matthew A. Booker, Michael Yevgeniy Tolstorukov, Sachiv Chakravarti, Conor Hinchey, et al.3 days after transduction using Vex reporter gene as a readout and zombie green as a viability dye #423111 (Biolegend London UK). CAR-T doses were calculated by percent transduction of viable cells. Cytotoxicity/cell

(20) Spliceosome inhibition induces Z-RNA and ZBP1-driven cell death in small cell lung cancerCell ReportsOctober 28, 2025Xinpei Jiang, Xueying Ma, Yunyun Zhou, Xiaodan Liu, Ting Zhang, William Kim, Siddharth Balachandran, Israel Cañadasand washed with PBS followed by 10 min incubation with Zombie Green Fixable Viability dye (BioLegend; 423112). Cells were washed with PBS and followed by 10 min fixation with 4% PFA in PBS (Thermo Fisher; J61899).

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(1)

10/17/2023Superb Kit From BioLegend for Live/Dead Cell Analysis Danielle SchmidtDoes a great job staining dead cells for accurate live/dead analysis in flow cytometry. Be sure to keep it in the dark, as it is light-sensitive!
(1)

7/22/2023Great Viability Dye for Flow Cytometry From BioLegend Troy TrevinoInflammation in brain endothelial cells is measured during viral respiratory infection in mice. Zombie Green viability dye was used to exclude dead cells from analysis.
(1)

7/4/2019Very Good BioLegend Kit for Viability QC on FACS Evaristus MbanefoIt is a good product and serves the purpose. I will give 9/10.

Zombie Green™ Fixable Viability Kit from BioLegend

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Zombie Green™ Fixable Viability Kit from BioLegend

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