Very Good BioLegend Kit for Viability QC on FACS

Bladder Immunology Group
Biomedical Research Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Zombie Green Fixable Viability kit

Catalog Number:

423111

Clone Number:

N/A

This reagent is a perfect addition for gating out dead cells during FACS analysis. Pro:Easy to use, Fixable. Cons: Blocks off the FITC channel.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes and cell lines

Primary Incubation

30 min, RT in the dark

Blocking Agent

TruStain FcX (anti-mouse CD16/32) for Fc blocking

Secondary Incubation

Not Applicable

Tertiary Incubation

Not Applicable

Detection

FACS

Results Summary

It accurately stains dead cells which can be gated out as positive signals on FITC channel.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Easy to use, Fixable, Sensitive, and generally efficient

The Bad

Blocks off the FITC channel

The Bottom Line

It is a good product and serves the purpose. I will give 9/10.

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