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Assay Kits
Assay Kit
NADP/NADPH Assay Kit (Fluorometric)
NADP/NADPH Assay Kit (Fluorometric) from Abcam
(3)
Write a review
Citations:
(17)
Supplier Page
Supplier Page from
Abcam for
NADP/NADPH Assay Kit (Fluorometric)
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
NADP/NADPH Assay Kit (Fluorometric)
Company
Abcam
Price
Supplier Page
View Company Product Page
Catalog Number
ab176724
Quantity
250 Test
Sample Type
Cell culture extracts, Tissue Extracts
Assay Time
1h 0m
Add to Compare List
Abcam
Discovery Drive
Cambridge Biomedical Campus
Cambridge
CB2 0AX
United Kingdom
Phone
: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600
Company Profile
Website:
http://www.abcam.com
Citations (17)
(1)
Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomer
Microbial Cell Factories
February 2, 2026
Annalena Sommer, Sarah Pauli, Michael Kohlstedt, Judith Becker, Christoph Wittmann
and NADPH The
NADP
⁺/NADPH ratio in selected
strains
was determined using the
NADP⁺/NADPH Assay Kit
(
ab176724
,
Abcam
, Cambridge, UK). Reagents and materials were prepared following the manufacturer’s protocol [18].
(2)
Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fraction
Cell Metabolism
July 1, 2025
Yuki Tatekoshi, Amir Mahmoodzadeh, Jason S. Shapiro, Mingyang Liu, George M. Bianco, Ayumi Tatekoshi, Spencer Duncan Camp, Adam De Jesus, Navid Koleini, Santiago De La Torre,
et al.
in cells overexpressing FL-
HK1
or Tr-
HK1
were measured using the
NADP/NADPH Assay Kit (Fluorometric
,
ab176724
Abcam
) along with the manufacturer’s instructions. Briefly, cells were treated with various concentrations
(3)
Neutrophil glucose flux as a therapeutic target in antiphospholipid syndrome
Journal of Clinical Investigation
August 1, 2024
Ajay Tambralli, Alyssa Harbaugh, Somanathapura K. NaveenKumar, Megan D. Radyk, Christine E. Rysenga, Kaitlyn Sabb, Julia M. Hurley, Gautam J. Sule, Srilakshmi Yalavarthi, Shanea K. Estes,
et al.
collected, snap frozen in liquid nitrogen, and stored at –80°C. G6P (
Abcam
,
ab107923
) and NADPH (
Abcam
,
ab176724
) were quantified according to the manufacturer’s instructions. Animal housing and treatments
Mice
(4)
The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphate
The EMBO journal
July 1, 2024
Bhattacharjee P, Wang D, Anderson D, Buckler JN, de Geus E, Yan F, Polekhina G, Schittenhelm R, Creek DJ, Harris LD,
et al
.
were measured by an enzyme cycling reaction by bioluminescence using a commercial
assay
kit (
Abcam
ab176724
) following the manufacturer protocol.
Assays
in mammalian cells Three separate murine
macrophage
cell
(5)
Neutrophil glucose flux as a therapeutic target in antiphospholipid syndrome
J Clin Invest
June 13, 2024
Ajay Tambralli, Alyssa Harbaugh, Somanathapura K. NaveenKumar, Megan D. Radyk, Christine E. Rysenga, Kaitlyn Sabb, Julia M. Hurley, Gautam J. Sule, Srilakshmi Yalavarthi, Shanea K. Estes,
et al.
collected, snap frozen in liquid nitrogen, and stored at –80°C. G6P (
Abcam
,
ab107923
) and NADPH (
Abcam
,
ab176724
) were quantified according to the manufacturer’s instructions. Animal housing and treatments
Mice
(6)
Adaptive changes in tumor cells in response to reductive stress
Biochemical Pharmacology
January 1, 2024
Leilei Zhang, Jie Zhang, Zhi-wei Ye, Aslam Muhammad, Li Li, John W. Culpepper, Danyelle M. Townsend, Kenneth D. Tew
NADP
+/NADPH levels in cells were measured using the colorimetric NAD+ /NADH (
Ab65348
) and
NADP
+/NADPH (
Ab176724
) kit
assays
, respectively, according to the manufacturer’s protocols (
Abcam
, Cambridge, United Kingdom).
(7)
Metabolic reprogramming contributes to radioprotection by protein kinase Cδ
Journal of Biological Chemistry
October 1, 2023
Angela M. Ohm, Trisiani Affandi, Julie A. Reisz, M. Cecilia Caino, Angelo D’Alessandro, Mary E. Reyland
min. NADPH/NADP+
assay
The NADPH/NADP+ ratio was measured using the NADPH/NADP+ Fluorometric
Assay
Kit (Cat #
ab176724
,
Abcam
) according to the manufacturer’s protocols. An equal number of cells from each cell line was
(8)
Succinate mediates inflammation-induced adrenocortical dysfunction
Elife
July 14, 2023
Ivona Mateska, Anke Witt, Eman Hagag, Anupam Sinha, Canelif Yilmaz, Evangelia Thanou, Na Sun, Ourania Kolliniati, Maria Patschin, Heba Abdelmegeed,
et al.
measurement Intracellular NADPH/
NADP
+ ratio was measured with the
NADP/NADPH Assay Kit (Fluorometric
) (
ab176724
,
Abcam
). NCI-H295R cells were plated at 5×106 cells/10 cm – diameter dish.
Fluorescence
(Ex/Em = 540/590
(9)
Insulin regulates neurovascular coupling through astrocytes
Proceedings of the National Academy of Sciences
July 20, 2022
Ana M. Fernandez, Laura Martinez-Rachadell, Marta Navarrete, Julia Pose-Utrilla, Jose Carlos Davila, Jaime Pignatelli, Sonia Diaz-Pacheco, Santiago Guerra-Cantera, Emilia Viedma-Moreno, Rocio Palenzuela,
et al.
Assays
. The NADPH/
NADP
ratio in
astrocytes
was calculated using a fluorometric
NADP
/NADPH
assay
(
Abcam
,
ab176724
). ROS levels in
shRNA
transfected
astrocytes
was determined using the fluorescent H2DCFDA (
Invitrogen
,
(10)
From macro to micro: a combined bioluminescence‐fluorescence approach to monitor bacterial localization
Environmental Microbiology
April 1, 2021
Riccardo Soldan, Nattapong Sanguankiattichai, Marcel Bach‐Pages, Indra Bervoets, Wei E. Huang, Gail M. Preston
rpm for 6 h. Cultures were processed according to the manufacturer's instructions (
NADP
/NADPH
assay
ab176724
,
Abcam
, UK) at a final OD600 of 3,3. Determining the effect of bacterial bioluminescence expression
(11)
Integrated Metabolomics and Targeted Gene Transcription Analysis Reveal Global Bacterial Antimonite Resistance Mechanisms
Frontiers in Microbiology
January 28, 2021
Jingxin Li, Yuxiao Zhang, Xing Wang, Seth T. Walk, Gejiao Wang
The detection of total
NADP
+ and NADPH was performed using the
NADP
+ /NADPH fluorometric
assay
kit (
ab176724
,
Abcam
) according to the manufacturer’s instructions.
Fluorescence
intensity was measured with a multimode
(12)
Inhibition of carnitine palmitoyl transferase 1A-induced fatty acid oxidation suppresses cell progression in gastric cancer
Archives of Biochemistry and Biophysics
December 1, 2020
Liqiang Wang, Changfeng Li, Yumei Song, ZhenKun Yan
instructions, using FAO Detection Kit (Genmed Scientifics, Shanghai, China) and
NADP/NADPH Assay Kit
(
ab176724
;
Abcam
) assayed FAO rate and the intracellular NADPH/
NADP
levels. BODIPY 493/503
Staining
GC cells
(13)
Dietary magnesium supplementation improves lifespan in a mouse model of progeria
EMBO Molecular Medicine
October 7, 2020
Ricardo Villa‐Bellosta
(GR) activity in both VSMCs and liver lysates were measured with commercials kit (
Abcam
,
ab138881
,
ab176724
, and
ab83461
, respectively), using a 96‐well plate reader, according to the manufacturer's protocols.
(14)
Myeloid FBW7 deficiency disrupts redox homeostasis and aggravates dietary-induced insulin resistance
Redox biology
October 1, 2020
Wang C, Chao Y, Xu W, Liu Z, Wang H, Huang K.
ratios Fresh cells were used immediately to determine the GSH/GSSG (
ab138881
,
Abcam
) and NADPH/
NADP
+ ratios (
ab176724
,
Abcam
) using commercially available kits. Protein concentrations of the cells lysates or actual tissue
(15)
The Role of Nonglycolytic Glucose Metabolism in Myocardial Recovery Upon Mechanical Unloading and Circulatory Support in Chronic Heart Failure
Circulation
July 21, 2020
Badolia R, Ramadurai DKA, Abel ED, Ferrin P, Taleb I, Shankar TS, Krokidi AT, Navankasattusas S, McKellar SH, Yin M,
et al
.
800CW
Donkey
anti-rabbit (no.
926–32213
) were from
LI-COR
(Lincoln, NE).
NADP/ NADPH Assay kit
(no.
ab176724
) were from
Abcam
(Cambridge, MA), and
OxyBlot Protein Oxidation Detection kit
(no.
S7150
) was from
Millipore Sigma
(16)
Cardiovascular phenotype of mice lacking 3-mercaptopyruvate sulfurtransferase
Biochemical pharmacology
June 1, 2020
Peleli M, Bibli SI, Li Z, Chatzianastasiou A, Varela A, Katsouda A, Zukunft S, Bucci M, Vellecco V, Davos CH,
et al
.
with the
RIPA buffer
. NADPH/
NADP
+ and GSH/GSSG ratios were measured using commercially available kits (
ab176724
and
ab138881
,
Abcam
, Cambridge, UK) according to the manufacturer’s instructions. Tissue weight was
(17)
Cyclin D1 unbalances the redox status controlling cell adhesion, migration, and drug resistance in myeloma cells
Oncotarget
July 19, 2016
Bustany S, Bourgeais J, Tchakarska G, Body S, Hérault O, Gouilleux F, Sola B.
determination of the NADPH/
NADP
+ ratio (from 106 cells) was performed using the
NADP+/NADPH Assay Kit
(
ab176724
,
Abcam
). This colorimetric
assay
was carried out according to the manufacturer's instructions. The optical
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Reviews
Write a Review
(1)
6/7/2023
Easy and Straightforward Kit to Detect NADP/NADPH
Wei Yang Sit
Glucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. The kit allows the ...
Read Review
(1)
6/6/2022
NADP/NADPH Assay Kit Worked Well
Lucy Liu
The goal of this research is to evaluate NADP, NADPH in mouse derived macrophages. We seed cells in 96-well plate and used this kit to detect NADP, NADPH and their ration. This kit worked well for ...
Read Review
(1)
6/17/2016
This Kit Did Not Work.
Netanya Spencer
The manual contains many mistakes and unclear instructions. However, I communicated with tech support at Abcam, and got clearer instructions. I then followed exactly what they said to do. I ...
Read Review
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NADP/NADPH Assay Kit (Fluorometric) from Abcam
Supplier Page
View Company Product Page
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