NADP/NADPH Assay Kit (Fluorometric) ab176724 from Abcam

Product Specs
ItemNADP/NADPH Assay Kit (Fluorometric)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab176724
Quantity250 Test
Sample TypeCell culture extracts, Tissue Extracts
Assay Time1h 0m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(1) Metabolic engineering of Corynebacterium glutamicum for high-yield de novo biosynthesis of 5-aminovaleramide, a promising bio-based monomerMicrobial Cell FactoriesFebruary 2, 2026Annalena Sommer, Sarah Pauli, Michael Kohlstedt, Judith Becker, Christoph Wittmannand NADPH The NADP⁺/NADPH ratio in selected strains was determined using the NADP⁺/NADPH Assay Kit (ab176724, Abcam, Cambridge, UK). Reagents and materials were prepared following the manufacturer’s protocol [18].

(2) Protein O-GlcNAcylation and hexokinase mitochondrial dissociation drive heart failure with preserved ejection fractionCell MetabolismJuly 1, 2025Yuki Tatekoshi, Amir Mahmoodzadeh, Jason S. Shapiro, Mingyang Liu, George M. Bianco, Ayumi Tatekoshi, Spencer Duncan Camp, Adam De Jesus, Navid Koleini, Santiago De La Torre, et al.in cells overexpressing FL-HK1 or Tr-HK1 were measured using the NADP/NADPH Assay Kit (Fluorometric, ab176724 Abcam) along with the manufacturer’s instructions. Briefly, cells were treated with various concentrations

(3) Neutrophil glucose flux as a therapeutic target in antiphospholipid syndromeJournal of Clinical InvestigationAugust 1, 2024Ajay Tambralli, Alyssa Harbaugh, Somanathapura K. NaveenKumar, Megan D. Radyk, Christine E. Rysenga, Kaitlyn Sabb, Julia M. Hurley, Gautam J. Sule, Srilakshmi Yalavarthi, Shanea K. Estes, et al.collected, snap frozen in liquid nitrogen, and stored at –80°C. G6P (Abcam, ab107923) and NADPH (Abcam, ab176724) were quantified according to the manufacturer’s instructions. Animal housing and treatments Mice

(4) The immune response to RNA suppresses nucleic acid synthesis by limiting ribose 5-phosphateThe EMBO journalJuly 1, 2024Bhattacharjee P, Wang D, Anderson D, Buckler JN, de Geus E, Yan F, Polekhina G, Schittenhelm R, Creek DJ, Harris LD, et al.were measured by an enzyme cycling reaction by bioluminescence using a commercial assay kit (Abcam ab176724) following the manufacturer protocol. Assays in mammalian cells Three separate murine macrophage cell

(5) Neutrophil glucose flux as a therapeutic target in antiphospholipid syndromeJ Clin InvestJune 13, 2024Ajay Tambralli, Alyssa Harbaugh, Somanathapura K. NaveenKumar, Megan D. Radyk, Christine E. Rysenga, Kaitlyn Sabb, Julia M. Hurley, Gautam J. Sule, Srilakshmi Yalavarthi, Shanea K. Estes, et al.collected, snap frozen in liquid nitrogen, and stored at –80°C. G6P (Abcam, ab107923) and NADPH (Abcam, ab176724) were quantified according to the manufacturer’s instructions. Animal housing and treatments Mice

(6) Adaptive changes in tumor cells in response to reductive stressBiochemical PharmacologyJanuary 1, 2024Leilei Zhang, Jie Zhang, Zhi-wei Ye, Aslam Muhammad, Li Li, John W. Culpepper, Danyelle M. Townsend, Kenneth D. TewNADP+/NADPH levels in cells were measured using the colorimetric NAD+ /NADH (Ab65348) and NADP+/NADPH (Ab176724) kit assays, respectively, according to the manufacturer’s protocols (Abcam, Cambridge, United Kingdom).

(7) Metabolic reprogramming contributes to radioprotection by protein kinase CδJournal of Biological ChemistryOctober 1, 2023Angela M. Ohm, Trisiani Affandi, Julie A. Reisz, M. Cecilia Caino, Angelo D’Alessandro, Mary E. Reylandmin. NADPH/NADP+ assay The NADPH/NADP+ ratio was measured using the NADPH/NADP+ Fluorometric Assay Kit (Cat # ab176724, Abcam) according to the manufacturer’s protocols. An equal number of cells from each cell line was

(8) Succinate mediates inflammation-induced adrenocortical dysfunctionElifeJuly 14, 2023Ivona Mateska, Anke Witt, Eman Hagag, Anupam Sinha, Canelif Yilmaz, Evangelia Thanou, Na Sun, Ourania Kolliniati, Maria Patschin, Heba Abdelmegeed, et al.measurement Intracellular NADPH/NADP+ ratio was measured with the NADP/NADPH Assay Kit (Fluorometric) (ab176724, Abcam). NCI-H295R cells were plated at 5×106 cells/10 cm – diameter dish. Fluorescence (Ex/Em = 540/590

(9) Insulin regulates neurovascular coupling through astrocytesProceedings of the National Academy of SciencesJuly 20, 2022Ana M. Fernandez, Laura Martinez-Rachadell, Marta Navarrete, Julia Pose-Utrilla, Jose Carlos Davila, Jaime Pignatelli, Sonia Diaz-Pacheco, Santiago Guerra-Cantera, Emilia Viedma-Moreno, Rocio Palenzuela, et al.Assays. The NADPH/NADP ratio in astrocytes was calculated using a fluorometric NADP/NADPH assay (Abcam, ab176724). ROS levels in shRNA transfected astrocytes was determined using the fluorescent H2DCFDA (Invitrogen,

(10) From macro to micro: a combined bioluminescence‐fluorescence approach to monitor bacterial localizationEnvironmental MicrobiologyApril 1, 2021Riccardo Soldan, Nattapong Sanguankiattichai, Marcel Bach‐Pages, Indra Bervoets, Wei E. Huang, Gail M. Prestonrpm for 6 h. Cultures were processed according to the manufacturer's instructions (NADP/NADPH assay ab176724, Abcam, UK) at a final OD600 of 3,3. Determining the effect of bacterial bioluminescence expression

(11) Integrated Metabolomics and Targeted Gene Transcription Analysis Reveal Global Bacterial Antimonite Resistance MechanismsFrontiers in MicrobiologyJanuary 28, 2021Jingxin Li, Yuxiao Zhang, Xing Wang, Seth T. Walk, Gejiao WangThe detection of total NADP+ and NADPH was performed using the NADP + /NADPH fluorometric assay kit (ab176724, Abcam) according to the manufacturer’s instructions. Fluorescence intensity was measured with a multimode

(12) Inhibition of carnitine palmitoyl transferase 1A-induced fatty acid oxidation suppresses cell progression in gastric cancerArchives of Biochemistry and BiophysicsDecember 1, 2020Liqiang Wang, Changfeng Li, Yumei Song, ZhenKun Yaninstructions, using FAO Detection Kit (Genmed Scientifics, Shanghai, China) and NADP/NADPH Assay Kit (ab176724; Abcam) assayed FAO rate and the intracellular NADPH/NADP levels. BODIPY 493/503 Staining GC cells

(13) Dietary magnesium supplementation improves lifespan in a mouse model of progeriaEMBO Molecular MedicineOctober 7, 2020Ricardo Villa‐Bellosta(GR) activity in both VSMCs and liver lysates were measured with commercials kit (Abcam, ab138881, ab176724, and ab83461, respectively), using a 96‐well plate reader, according to the manufacturer's protocols.

(14) Myeloid FBW7 deficiency disrupts redox homeostasis and aggravates dietary-induced insulin resistanceRedox biologyOctober 1, 2020Wang C, Chao Y, Xu W, Liu Z, Wang H, Huang K.ratios Fresh cells were used immediately to determine the GSH/GSSG (ab138881, Abcam) and NADPH/NADP + ratios (ab176724, Abcam) using commercially available kits. Protein concentrations of the cells lysates or actual tissue

(15) The Role of Nonglycolytic Glucose Metabolism in Myocardial Recovery Upon Mechanical Unloading and Circulatory Support in Chronic Heart FailureCirculationJuly 21, 2020Badolia R, Ramadurai DKA, Abel ED, Ferrin P, Taleb I, Shankar TS, Krokidi AT, Navankasattusas S, McKellar SH, Yin M, et al.800CW Donkey anti-rabbit (no. 926–32213) were from LI-COR (Lincoln, NE). NADP/ NADPH Assay kit (no. ab176724) were from Abcam (Cambridge, MA), and OxyBlot Protein Oxidation Detection kit (no. S7150) was from Millipore Sigma

(16) Cardiovascular phenotype of mice lacking 3-mercaptopyruvate sulfurtransferaseBiochemical pharmacologyJune 1, 2020Peleli M, Bibli SI, Li Z, Chatzianastasiou A, Varela A, Katsouda A, Zukunft S, Bucci M, Vellecco V, Davos CH, et al.with the RIPA buffer. NADPH/NADP+ and GSH/GSSG ratios were measured using commercially available kits (ab176724 and ab138881, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Tissue weight was

(17) Cyclin D1 unbalances the redox status controlling cell adhesion, migration, and drug resistance in myeloma cellsOncotargetJuly 19, 2016Bustany S, Bourgeais J, Tchakarska G, Body S, Hérault O, Gouilleux F, Sola B.determination of the NADPH/NADP+ ratio (from 106 cells) was performed using the NADP+/NADPH Assay Kit (ab176724, Abcam). This colorimetric assay was carried out according to the manufacturer's instructions. The optical

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(1)

6/7/2023Easy and Straightforward Kit to Detect NADP/NADPH Wei Yang SitGlucose 6-phosphate (G6P) is oxidized to 6-phosphate gluconolactone (6-PG) by glucose-6-phosphate dehydrogenase (G6PDH), and NADP+ is reduced to NADPH during this reaction. The kit allows the ... Read Review
(1)

6/6/2022NADP/NADPH Assay Kit Worked Well Lucy LiuThe goal of this research is to evaluate NADP, NADPH in mouse derived macrophages. We seed cells in 96-well plate and used this kit to detect NADP, NADPH and their ration. This kit worked well for ... Read Review
(1)

6/17/2016This Kit Did Not Work. Netanya SpencerThe manual contains many mistakes and unclear instructions. However, I communicated with tech support at Abcam, and got clearer instructions. I then followed exactly what they said to do. I ... Read Review

NADP/NADPH Assay Kit (Fluorometric) from Abcam

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NADP/NADPH Assay Kit (Fluorometric) from Abcam

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