ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(41) Conversion of IscB and Cas9 into RNA-guided RNA editorsCellOctober 16, 2025Chengtao Xu, Xiaolin Niu, Haifeng Sun, Hao Yan, Weixin Tang, Ailong Ke(Invitrogen) and reverse-transcribed to cDNAs with ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560). Quantitative PCR (qPCR) was performed with the cDNA for each sample on a CFX96 real-time system (Biorad)

(42) RNF114 and RNF166 exemplify reader-writer E3 ligases that extend K11 polyubiquitin onto sites of MARUbylationThe EMBO JournalOctober 2, 2025Rachel E Lacoursiere, Kapil Upadhyaya, Jasleen Kaur Sidhu, Ivan Rodriguez Siordia, Daniel S Bejan, Michael S Cohen, Jonathan N Prunedastudy,N/A NucleoSpin RNA Plus Kit,Macherey-Nagel,NC1749027 ProtoScript II First Strand cDNA Synthesis Kit,NEB,E6560S PowerUp SYBR green Master Mix,ThermoFisher Scientific,A25742 MgCl2,Fisher Scientific,M33-500 Dithiothreitol (DTT

(43) Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality controlNature structural & molecular biologyOctober 1, 2025Rodschinka G, Forcelloni S, Kühner FM, Wani S, Riemenschneider H, Edbauer D, Behrens A, Nedialkova DD.mM deoxynucleotide triphosphates, 10 mM DTT, 20 U of Superase-In and 200 U of Protoscript II (NEB, E6560S). The RNA was hydrolyzed by addition of 0.1 M NaOH and incubation for 5 min at 90 °C and cDNA products

(44) Genotype-by-Inhibitor Interactions to Dissect Enterovirus ReplicationResearch SquareSeptember 30, 2025Patrick Bakhache, William Symonds-Orr, Patrick T. Symonds-OrrQIAGEN RNeasy kit (QIAGEN, 74106). ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L) was used to generate first strand cDNA using a reverse primer in the 3’UTR, TGGTTATAACAAATTTACCCCCACCA.

(45) Dynamic regulation of the oxidative stress response by the E3 ligase TRIP12Cell ReportsSeptember 23, 2025Andrew J. Ingersoll, Devlon M. McCloud, Jenny Y. Hu, Michael Rapeof cDNA prepared from HEK293T cells using the Protoscript II First Strand cDNA Synthesis Kit (NEB, E6560S). Antibodies The following antibodies were used in this study: αNRF2 (Cell Signaling, 12721S), α KEAP1

(46) Metabolite-enhanced normothermic machine perfusion improves kidney transplant viabilityJCI InsightSeptember 23, 2025Jan Czogalla, Fabian Hausmann, Simon Lagies, Sydney E. Gies, Sabrina Christiansen, Nico Kaiser, Fabian Haas, Yusuke Okabayashi, Dominik Kylies, Smilla Hofmann, et al.used for DNA digestion. For reverse transcription, Protoscript II First Strand cDNA Synthesis Kit (E6560L, New England BioLabs) was used. qPCR was performed on a QuantStudio 3 System (A28566, Thermo Fisher

(47) Enhancing RNA base editing on mammalian transcripts with small nuclear RNAsNature Chemical BiologySeptember 18, 2025Aaron A. Smargon, Deepak Pant, Trent A. Gomberg, Christian Fagre, Sofia Glynne, Johnathan Nguyen, Jack T. Naritomi, Wendy V. Gilbert, Gene W. Yeoprecipitation. cDNA synthesis was carried out using the ProtoScript II first-strand cDNA synthesis kit (E6560L, New England Biolabs) with 6 μl of RNA in a 20-μl total volume using random hexamer primers supplied

(48) Regulated Release of Small Extracellular Vesicles Directs Neutrophil Recruitment in Cutaneous Wound HealingJournal of Investigative DermatologySeptember 4, 2025Wooil Choi, Dong Jun Park, Robert A. Dorschner, Katie Pool, Sakeef Sayeed, Jenny Kezios, Jaebin Lee, Sebastian Adlawan, Brian P. Eliceirisynthesized using a cDNA synthesis kit (ProScript II first strand cDNA synthesis kit, catalog number E6560, New England Biolabs). qPCR experiments were carried out by a real-time PCR detection system (CFX connect

(49) Targeting SUMO2 reverses aberrant epigenetic rewiring driven by SS18::SSX fusion oncoproteins and impairs sarcomagenesisThe EMBO JournalAugust 13, 2025Rema Iyer, Anagha Deshpande, Aditi Pedgaonkar, Pramod Akula Bala, Taehee Kim, Gerard L Brien, Darren Finlay, Kristiina Vuori, Alice Soragni, Hiromi I Wetterstein, et al.Propidium iodide,ThermoFisher,00-6990-50 Trizol,ThermoFisher,15596026 Lipofectamine 2000,ThermoFisher,11668500 Protoscript II,NEB,E6560S RIPA lysis buffer,ThermoFisher,89900 protease inhibitor cocktail,ThermoFisher,78429 LDS Sample buffer,ThermoFisher,J61942.AD 10X

(50) Natural SEL1L variants rescue a model of NGLY1 deficiency and modify ERAD function and proteasome sensitivityPLoS GenetAugust 7, 2025Travis K. Tu'ifua, Clement Y. ChowDNAse step. RNA was converted to cDNA using a ProtoScript II First Strand cDNA Synthesis Kit (NEB Cat # E6560L). RT-qPCR was performed using a QuantStudio 3 96-well 0.2 ml block instrument and PowerUp SYBR Green Master Mix

(51) Reduced Sphingosine-1-Phosphate Levels Exacerbate Type 2 Diabetes Mellitus and Associated Complications in a High-Fat Diet Mouse ModelEndocrinol Metab (Seoul)August 5, 2025Shih-Chang Hsu, Ching-Lu Chen, Chung Te Liu, Hung-Chieh Lo, Ying-Kuo Liu, Pei-Song Gao, Shau-Ku Huang, Chin-Wang Hsutotal RNA was reverse-transcribed into cDNA using the ProtoScript® First Strand cDNA Synthesis Kit (#E6560L; New England Biolabs). Quantitative real-time PCR (qRT-PCR) was performed using Fast SYBRTM Green Master

(52) Large-scale RNA-Seq mining reveals ciclopirox olamine induces TDP-43 cryptic exonsNat CommunJuly 25, 2025Irika R. Sinha, Parker S. Sandal, Holly Spence, Grace D. Burns, Aswathy Peethambaran Mallika, Fatemeh Abbasinejad, Katherine E. Irwin, Anna Lourdes F. Cruz, Vania Wang, Shaelyn R. Marx, et al.cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L). cDNA was amplified using primer pairs designed to target either two wildtype sequences flanking the

(53) Canalization of flower production across thermal environments requires Florigen and CLAVATA signalingCurrent BiologyJuly 21, 2025Elizabeth S. Smith, Amala John, Andrew C. Willoughby, Daniel S. Jones, Vinicius C. Galvão, Christian Fankhauser, Zachary L. NimchukResearch Center) BN 191, Glycoblue,Thermo,AM9515 Protoscript ii First Strand cDNA synthesis kit,NEB,E6560L 17-505B qPCRBIO SyGreen Blue Mix Lo-RO 5 x 1mL Master Mix,Genesee,17-505B BpiI,Thermo,FD1014 T4 DNA ligase

(54) Orphan receptor GPR153 facilitates vascular damage responses by modulating cAMP levels, YAP/TAZ signaling, and NF-κB activationNat CommunJuly 7, 2025Jingchen Shao, Jeonghyeon Kwon, Tianpeng Wang, Stefan Günther, Lukas S. Tombor, Timothy Warwick, Zaib Shaheryar, Ralf P. Brandes, Stefanie Dimmeler, Jan Wenzel, et al.reverse transcription were performed using the Quick RNA Micro kit (R1051; Zymo) and ProtoScriptII (E6560; New England Biolabs) according to the manufacturer’s instruction. Gene expression levels were determined

(55) An endothelial specific mouse model for the capillary malformation mutation Gnaq p.R183QAngiogenesisJune 11, 2025Patrick Smits, Leanna Marrs, Yu Sheng Cheng, Michal Ad, Sana Nasim, David Zurakowski, Joyce Bischoff, Arin K. Greeneperformed by generating cDNA with the Protoscript II kit using the oligo-dT primer (New England Biolabs: E6560) and then performing ddPCR to detect wild-type and missense mutant transcripts. ddPCR primers and probes

(56) MyD88 knockdown by RNAi prevents bacterial stimulation of tubeworm metamorphosisProc Natl Acad Sci U S AJune 10, 2025Emily Darin, Morgan V. Farrell, Tatyana N. Ali, Josefa Rivera Alfaro, Kyle E. Malter, Nicholas J. Shikumaperformed on RNA samples using NEB biolabs Protoscript II First Strand cDNA Synthesis kit (Catalog # E6560S). Each cDNA reaction was standardized to synthesize 1 µg of RNA. Reactions were carried out at 42 °C

(57) Activation of intestinal mast cells contributes to gut damage after cardiac arrest in miceShockJune 6, 2025Lihong Dang, Ata Ur Rehman, Jin Zhang, Ran Zhang, Xinyuan Yu, Huaxin Sheng, Jörn Karhausen, Wei YangUSA), and cDNA was obtained using ProtoScript II first strand cDNA Synthesis Kit (New England Biolabs; E6560), both according to the manufacturer’s instructions. Quantitative PCR was performed using iTaq Universal

(58) Short-Term Evolutionary Features and Circadian Clock-Modulated Gene Expression Analysis of Piezo, nanchung, and αTubulin at 67C in a Romanian Population of Drosophila suzukiiInsectsJune 4, 2025Adriana-Sebastiana Musca, Attila Cristian Ratiu, Adrian Ionascu, Nicoleta-Denisa Constantin, Marius Zahanwas conducted using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, cat. no. E6560), allowing the conversion of 1000 ng of RNA into the corresponding cDNA. For qRT-PCR reactions, three

(59) Bile acids target an exposed cavity in the glucocorticoid receptor modulating receptor self-assembly, chromatin binding and transcriptional activityBIORXIVMay 16, 2025Alba Jiménez-Panizo, Thomas A. Johnson, Kaustubh Wagh, Andrea Alegre-Martí, Inés Montoya Novoa, Agustina L. Lafuente, Ulrich Eckhard, Luis Ángel Rodríguez-Lumbreras, Le Hoang, Martín Stortz, et al.#12183018 A; Thermo Fisher Scientific) and cDNA reactions were performed with the NEB Protoscript II kit (#E6560S) following the manufacturers’ instructions. RT-qPCRs were performed with iQ SYBR Green Supermix (Biorad

(60) Genotype-specific differences in infertile men due to loss-of-function variants in M1AP or ZZS genesEMBO Molecular MedicineMay 15, 2025Nadja Rotte, Jessica E M Dunleavy, Michelle D Runkel, Lina Bosse, Daniela Fietz, Adrian Pilatz, Johanna Kuss, Ann-Kristin Dicke, Sofia B Winge, Sara Di Persio, et al.transcribed into cDNA using the ProtoScript® II first strand cDNA synthesis kit (New England Biolabs, #E6560). Amplification was conducted with primers annealing to rat insulin exon 3 and exon 4, respectively.

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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