ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

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Website: http://www.neb.com

(61) Crosslinking stabilization strategy: A novel approach to cartilage-like repair of annulus fibrosus (AF) defectsMater Today BioMarch 5, 2025Zihan Wang, Lei Wang, Shaoshuo Li, Xin Chen, Bo Chen, Zhichao Lou, Zheng Li, Rongrong Deng, Lin Xie, Jianwei Wang, et al.(15596026, Invitrogen, USA) following the manufacturer's instructions. A reverse transcription kit (E6560, NEB, USA) was used to synthesize cDNA from the RNA, followed by qPCR analysis using SYBR Premix (E3003,

(62) Improving adenine base editing precision by enlarging the recognition domain of CRISPR-Cas9Nature CommunicationsFebruary 28, 2025Shuliang Gao, Benson Weng, Douglas Wich, Liam Power, Mengting Chen, Huiwen Guan, Zhongfeng Ye, Chutian Xu, Qiaobing XucDNA synthesis was performed using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560) with 1 µg of RNA in a total reaction volume of 20 µl. Reactions were incubated for 2 hr at 42 °C. 2

(63) Phospho-relay feedback loops control egress vs. intracellular development in Toxoplasma gondiiCell ReportsFebruary 25, 2025Ja E. Claywell, Yong Fu, L. David SibleyKit,Qiagen,Cat#69504 RNeasy Mini Kit,Qiagen,Cat#74104 ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat#E6560 PDE-Glo Phosphodiesterase Assay,Promega,Cat# V1361 Cyclic GMP Direct ELISA Kit,Arborassays,Cat#K065 Cyclic

(64) Experimental horizontal transfer of phage-derived genes to Drosophila confers innate immunity to parasitoidsCurrent BiologyFebruary 3, 2025Rebecca L. Tarnopol, Josephine A. Tamsil, Gyöngyi Cinege, Ji Heon Ha, Kirsten I. Verster, Edit Ábrahám, Lilla B. Magyar, Bernard Y. Kim, Susan L. Bernstein, Zoltán Lipinszki, et al.,New England Biolabs,Catalog #M0491S Protoscript II First Strand cDNA Synthesis Kit,New England Biolabs,Catalog #E6560S NEBuilder HiFi DNA Assembly Master Mix,New England Biolabs,Catalog #E2621S pENTR/D-TOPO Cloning Kit,Invitrogen,Catalog

(65) The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcomaNature communicationsFebruary 2, 2025Banday S, Mishra AK, Rashid R, Ye T, Ali A, Li J, Yustein JT, Kelliher MA, Zhu LJ, Deibler SK, et al.(Invitrogen, Cat# 15596). cDNA was synthesized using Proto Script II reverse transcription kit (NEB, Cat# E6560) and real-time PCR reactions were performed and analyzed using a Quant Studio 3 real-time PCR system

(66) Metallothionein II treatment mitigates rotenone-induced neurodegeneration in zebrafish models of Parkinson’s diseaseFrontiers in PharmacologyJanuary 31, 2025Yong Hui Nies, Wei Ling Lim, Norwahidah Abd Karim, Mohamad Fairuz Yahaya, Seong Lin Teohsynthesized from 500 ng of the total RNA using ProtoScript II First Strand cDNA Synthesis Kit (Cat #E6560L, New England BioLabs, Ipswich, MA, United States) in a 20 µL reaction mixture, according to the manufacturer’s

(67) Probing the functional constraints of influenza A virus NEP by deep mutational scanningCell ReportsJanuary 28, 2025Qi Wen Teo, Yiquan Wang, Huibin Lv, Michael S. Oade, Kevin J. Mao, Timothy J.C. Tan, Yang Wei Huan, Joel Rivera-Cardona, Evan K. Shao, Danbi Choi, et al.Mini Kit,Qiagen,Cat# 74104 ProtoScript II First Strand cDNA Synthesis Kit,New England Biolabs,Cat# E6560L Superscript III reverse transcriptase,Thermo Fisher Scientific,Cat# 18080044 RNase inhibitor,ApexBio,Cat#

(68) Identification of glutamine as a potential therapeutic target in dry eye diseaseSignal Transduct Target TherJanuary 22, 2025Xiaoniao Chen, Chuyue Zhang, Fei Peng, Lingling Wu, Deyi Zhuo, Liqiang Wang, Min Zhang, Zhaohui Li, Lei Tian, Ying Jie, et al.using TRIzol and reversely transcribed to cDNA using ProtoScript® II First Strand cDNA Synthesis Kit (E6560S, NEB) according to the manufacturer’s instructions. RT-qPCR was conducted using PowerUp SYBR Green Master Mix

(69) A conserved element in the first intron of Cd4 has a lineage specific, TCR signal-responsive, canonical enhancer function that matches the timing of cell surface CD4 upregulation required to prevent lineage choice errorFront ImmunolJanuary 15, 2025Gregory A. Swan, Chika Fujii, Mia E. Guzynski, Sheridan M. Page, Isabelle V. Meyers, Yordan P. Penev, Sejiro Littleton, Adinda Azzahra, Christine Richardson, Sophia D. SarafovaResearch R2060), then converted into cDNA using the Protoscript II First Strand cDNA Synthesis Kit (NEB #E6560S). Rpl13 was used as the internal reference gene, and quantitative PCR was completed using Thermo Scientific’s

(70) SMAD5 as a novel gene for familial pulmonary arterial hypertensionClinical ScienceJanuary 15, 2025Ding Cao, Ekkehard Grünig, Yuriy Sirenko, Ganna Radchenko, Henning Gall, Ayat Ahmed, Susanne Theiß, Mareike Lankeit, Benjamin Meder, Magdalena Laugsch, et al.Analytik Jena, Germany). The first chain of copy DNA (cDNA) was created by First Strand cDNA Synthesis Kit (E6560L, NEB, U.S.A.), and messenger ribonucleic acid (mRNA) was measured by quantitative polymerase chain reaction

(71) Expression of the IGF‑1Ea isoform in human placentas from third trimester normal and idiopathic intrauterine growth restriction singleton pregnancies: Correlations with clinical and histopathological parametersMol Med RepJanuary 9, 2025Apostolos Fasoulopoulos, Michail Varras, Fani-Niki Varra, Anastasios Philippou, Despina Myoteri, Viktoria-Konstantina Varra, Evgenia Kouroglou, Alexandros Gryparis, Argyro Papadopetraki, Iakovos Vlachos, et al.First Strand cDNA synthesis kit according to the manufacturer's instructions (New England BioLabs; cat. no. NE, E6560L). Quantitative PCR (qPCR) Oligonucleotide sequences were as follows: IGF-1Ea Forward, 5′-GTGGAGACAGGGGCTTTTATTTC-3′

(72) rbfox1 loss of function in zebrafish leads to dysregulation in bdnf/trkb2 and pac1a expression resulting in HPI axis hyperactivation, altered stress response and allostatic overloadbioRxivJanuary 9, 2025Adele Leggieri, Judit Judit García-Gonzalez, Saeedeh Hosseinian, Peter Ashdown, Sofia Anagianni, Xian Wang, William Havelange, Noèlia Fernàndez-Castillo, Bru Cormand, Caroline Helen Brennanwas reverse transcribed to cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (NEB, Cat.E6560) following manufacturer’s instructions. Resulting cDNA yield and quality were evaluated using BioDrop

(73) Rapid and quantitative functional interrogation of human enhancer variant activity in live miceNat CommunJanuary 6, 2025Ethan W. Hollingsworth, Taryn A. Liu, Joshua A. Alcantara, Cindy X. Chen, Sandra H. Jacinto, Evgeny Z. Kvonwere constructed from isolated RNA with the ProtoScript® II First Strand cDNA Synthesis Kit (NEB, #E6560) using Oligo(d)T primers and following the manufacturer’s recommended protocol. Quantitative PCR Quantitative PCR

(74) G-quadruplex stabilization provokes DNA breaks in human PKD1, revealing a second hit mechanism for ADPKDNat CommunJanuary 2, 2025Agata M. Parsons, Seth Byrne, Jesse Kooistra, John Dewey, Aaron L. Zebolsky, Gloria Alvarado, Gerrit J. Bouma, Gregory B. Vanden Heuvel, Erik D. Larsonkit (NEB, T2010S) and 500 ng was converted to cDNA with a Protoscript cDNA kit (New England Biolabs, E6560S). One microliter of cDNA was used in qPCR with primers specific to hPKD1 and mPkd1. The amount of PKD1

(75) PKMζ, a Brain-specific PKCζ Isoform, is Required for Glycolysis and Myofibroblastic Activation of Hepatic Stellate CellsCellular and molecular gastroenterology and hepatologyJanuary 1, 2025Wang X, Wang Y, Bai B, Shaha A, Bao W, He L, Wang T, Kitange GJ, Kang N.154025593, Qiagen). Reverse transcription was done with a ProtoScript II First Strand cDNA Synthesis Kit (E6560 Lot# 10208713, New England Biolabs), and PCR was completed with Phire Tissue Direct PCR Mater Mix Kit

(76) Omics-driven onboarding of the carotenoid producing red yeast Xanthophyllomyces dendrorhous CBS 6938Applied Microbiology and BiotechnologyDecember 28, 2024Emma E. Tobin, Joseph H. Collins, Celeste B. Marsan, Gillian T. Nadeau, Kim Mori, Anna Lipzen, Stephen Mondo, Igor V. Grigoriev, Eric M. Youngconverted to cDNA using the Protoscript II First-Strand cDNA Synthesis Kit (NEB, Ipswich, MA, USA, E6560S) following the supplier’s standard protocol instructions. RT-qPCR was then performed using IDT’s PrimeTime Gene Expression Master Mix

(77) Cellular retinoic acid-binding proteins regulate germ cell proliferation and sex determination in zebrafishDevelopment (Cambridge, England)December 15, 2024Fung L, Dranow DB, Subramanian A, Libby N, Schilling TF.input to produce cDNA using the ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). cDNA was diluted 1:25 in water and used as template in PCR reactions with Luna Universal qPCR Master Mix

(78) Caenorhabditis elegans inositol hexaphosphate pathways couple to RNA interference and pathogen defenseProceedings of the National Academy of SciencesDecember 3, 2024Wenjing Xu, Yifan Sun, Peter Breen, Gary Ruvkun, Kai Maoreverse transcribed using the ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs, E6560L). qPCR was carried out with iQ™ SYBR® Green Supermix (BIO-RAD, 1708880). For quantification, C. elegans

(79) The impact of S2 mutations on Omicron SARS-CoV-2 cell surface expression and fusogenicityEmerging microbes & infectionsDecember 1, 2024Escalera A, Laporte M, Turner S, Karakus U, Gonzalez-Reiche AS, van de Guchte A, Farrugia K, Khalil Z, van Bakel H, Smith D, et al.Briefly, cDNA synthesis was performed with random hexamers and ProtoScript II (New England Biolabs, cat. E6560) using 7 μL of RNA according to manufacturer’s recommendations. The RT reaction was incubated for 30

(80) Mutations in fibronectin dysregulate chondrogenesis in skeletal dysplasiaCellular and Molecular Life SciencesDecember 1, 2024Neha E. H. Dinesh, Justine Rousseau, Deane F. Mosher, Mike Strauss, Jeannie Mui, Philippe M. Campeau, Dieter P. Reinhardtof total RNA using the Protoscript First Strand cDNA Synthesis kit (New England Biolabs; Catalogue #E6560L) as per the manufacturer’s instructions. For real-time qPCR, experiments were performed using an SYBR

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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