ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(261) Rapid in-country sequencing of whole virus genomes to inform rabies elimination programmesWellcome Open ResearchJanuary 7, 2020Kirstyn Brunker, Gurdeep Jaswant, S.M. Thumbi, Kennedy Lushasi, Ahmed Lugelo, Anna M. Czupryna, Fred Ade, Gati Wambura, Veronicah Chuchu, Rachel Steenson, et al.2019d). Initially, extracted RNA was reverse transcribed using Protoscript II First Strand Sequencing kit (E6560, NEB) under manufacturer’s instructions. At later stages this was changed to Lunascript RT Supermix

(262) Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic SteatohepatitisCell metabolismJanuary 7, 2020Sun X, Seidman JS, Zhao P, Troutman TD, Spann NJ, Que X, Zhou F, Liao Z, Pasillas M, Yang X, et al.,Thermo Fisher Scientific,BP3815 DSG,Peirce,PI20593 ATP,Roche,R0441 GTP,Roche,R0461 CTP,Roche,R0451 Postscript II reagents,NEB,E6560S T4 RNA ligase 2 truncated,NEB,M0242S SUPERase,Ambion,AM2696 RNA 5’-Pyrophosphohydrolase,NEB,M0356S NEB

(263) Inhibition of Microglial TGFβ Signaling Increases Expression of Mrc1Frontiers in cellular neuroscienceJanuary 1, 2020von Ehr A, Attaai A, Neidert N, Potru PS, Ruß T, Zöller T, Spittau B.sample was reverse-transcribed to cDNA using the Protoscript® II First Strand cDNA Synthesis Kit (#E6560S, New England Biolabs, Frankfurt, Germany) according to the manufacturer’s instructions. Quantitative

(264) Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor-Dependent R-Loops and DNA Damage in Breast Epithelial Cells and MiceEnvironmental health perspectivesJanuary 1, 2020Majhi PD, Sharma A, Roberts AL, Daniele E, Majewski AR, Chuong LM, Black AL, Vandenberg LN, Schneider SS, Dunphy KA, et al.RNA in 20 lL reaction mix with Protoscript II First Strand cDNA Synthesis Kit (New England Biolabs #E6560S), following the standard protocol provided by the manufacturer. qPCR for TFF1, progesterone receptor

(265) BRD4 bimodal binding at promoters and drug-induced displacement at Pol II pause sites associates with I-BET sensitivityEpigenetics & ChromatinDecember 1, 2019P. Khoueiry et al.using the Rneasy mini kit (Qiagen, #74104). cDNA was synthesized using the Protoscript 2 kit (NEB, #E6560S) according to manufacturer’s specifications. qPCR was performed in duplicate using 0.9 µl cDNA per reaction,

(266) Maturation-driven transport and AP-1–dependent recycling of a secretory cargo in the GolgiJournal of Cell BiologyMay 6, 2019Jason C. Casler, Effrosyni Papanikou, Juan J. Barrero, Benjamin S. GlickcDNA synthesis was performed using the ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs (E6560S). Briefly, 6 µl of the isolated RNA was combined with 10 µl of 2× ProtoScript II Reaction Mix, 2 µl

(267) Mitochondrial Dysfunction in C. elegans Activates Mitochondrial Relocalization and Nuclear Hormone Receptor-Dependent Detoxification GenesCell MetabolismMay 1, 2019Kai Mao, Fei Ji, Peter Breen, Aileen Sewell, Min Han, Ruslan Sadreyev, Gary Ruvkun#15596026). The cDNA was generated by ProtoScript® II First Strand cDNA Synthesis Kit (New England BioLabs, #E6560L). qPCR was performed toward interested genes with act-1 as control by iQ™ SYBR® Green Supermix (BIO-RAD,

(268) Comprehensive Genetic Characterization of Mitochondrial Ca²⁺ Uniporter Components Reveals Their Different Physiological Requirements In VivoCell reportsApril 30, 2019Tufi R, Gleeson TP, von Stockum S, Hewitt VL, Lee JJ, Terriente-Felix A, Sanchez-Martinez A, Ziviani E, Whitworth AJ.synthesized from total RNA using ProtoScript® II first strand cDNA Synthesis Kit (New England BioLabs, E6560S) according to manufacturer’s instructions. Total RNA concentration was ascertained spectrophotometrically,

(269) The proteasome biogenesis regulator Rpn4 cooperates with the unfolded protein response to promote ER stress resistanceeLifeMarch 13, 2019Rolf M Schmidt, Julia P Schessner, Georg HH Borner, Sebastian SchuckPierce Cat#23225 Commercial assay or kit ProtoScript II cDNA Synthesis kit New England Biolabs Cat#E6560L Commercial assay or kit SensiFAST SYBR No-ROX kit Bioline Cat#BIO-98050 Chemical compound, drug ß-estradiol Sigma-Aldrich Cat#E8875; CAS:

(270) Histone concentration regulates the cell cycle and transcription in early developmentDevelopmentFebruary 26, 2019Sudarshan Wilky, Sudarshan Chari, Jayalakshmi Govindan, Amanda A. AmodeoProtoScript First Strand cDNA Synthesis Kit following the manufacturer’s protocol (New England Biolabs, E6560L). qPCR was conducted on an ABI ViiA7 using the FG Taqman GEX master mix (Thermo Fisher Scientific, 4369016)

(271) TET1 regulates fibroblast growth factor 8 transcription in gonadotropin releasing hormone neuronsPloS oneJanuary 1, 2019Linscott ML, Chung WCJ.Micro-Volume plate adapter (Biotek). ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L). ProtoScript II First Strand cDNA Synthesis Kit was used to reverse transcribe 0.5 μg of total RNA.

(272) Insulin-Like Growth Factor Binding Protein 5-A Probable Target of Kidney Renal Papillary Renal Cell CarcinomaBioMed research internationalJanuary 1, 2019Wang S, Hong Q, Geng X, Chi K, Cai G, Wu D.(Peprotech, AF-100-21C). 2.3.4. qPCR Verification A First Strand cDNA Synthesis Kit (New England Biolabs, E6560S) was used for cDNA synthesis with a standard protocol. First, the RNA sample (up to 1 µg) was mixed

(273) Mechanical force regulates tendon extracellular matrix organization and tenocyte morphogenesis through TGFbeta signalingeLifeNovember 26, 2018Subramanian A, Kanzaki LF, Galloway JL, Schilling TF.SCR_016224 Commercial assay or kit Protoscript II first strand cDNA synthesis kit New England Biolabs Cat# E6560, RRID: SCR_016225 Commercial assay or kit Luna universal qPCR master mix New England Biolabs Cat#

(274) Esophageal IgG4 levels correlate with histopathologic and transcriptomic features in eosinophilic esophagitisAllergySeptember 1, 2018Chen E Rosenberg et al.immunoglobulin quantitation. RNA (500 ng) was reverse transcribed using ProtoScript cDNA Synthesis Kit (E6560, New England BioLabs, Ipswich, MA), according to the manufacturer’s protocol. Transcriptomic analysis Author

(275) Microglia-Specific Expression of Olfml3 Is Directly Regulated by Transforming Growth Factor β1-Induced Smad2 SignalingFrontiers in ImmunologyJuly 26, 2018Nicolas Neidert, Alexander von Ehr, Tanja Zöller, Björn Spittaueach sample was reverse transcribed to cDNA using Protoscript® II First Strand cDNA Synthesis Kit (#E6560S, New England Biolabs, Frankfurt, Germany) according to the manufacturer’s instructions. Quantitative

(276) RNA isolation from Peyer's patch lymphocytes and mononuclear phagocytes to determine gene expression profiles using NanoString technologyJournal of Biological MethodsJuly 2, 2018Navjot Singh, Heather C. Gallagher, Renjie Song, Jaskiran K. Dhinsa, Gary R. Ostroff, Magdia De Jesus(New England Biolabs, cat. # M3003X)ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, cat. # E6560S)DNA Oglios (IDT, # See Fig. S1 for sequences)Gold Taq Flexi DNA polymerase (Promega, cat. # M8295)Deoxynucleotide (dNTP) Solution Set

(277) CRISPR/Cas9 genome editing in wheatFunctional & Integrative GenomicsJanuary 1, 2018Dongjin Kim, Burcu Alptekin, Hikmet BudakCat# SLBR4100) and reverse-transcribed to cDNA using ProtoScript II first-strand cDNA synthesis kit (NEB Cat# E6560) following the manufacturer ’s suggestion. Quantitative real-time PCR was performed using a BioRad CFX96

(278) Comparison of the human gastric microbiota in hypochlorhydric states arising as a result of Helicobacter pylori-induced atrophic gastritis, autoimmune atrophic gastritis and proton pump inhibitor usePLOS PathogensNovember 2, 2017Bryony N. Parsons et al.before Proto reaction mix and Proto enzyme from a ProtoScript II First Strand cDNA Synthesis kit (NEB, E6560L) were added. Samples were then incubated at 25˚C for 5 minutes, 42˚C for 20 minutes, and 80˚C for 5

(279) Reduced FAK-STAT3 signaling contributes to ER stress-induced mitochondrial dysfunction and death in endothelial cellsCellular signallingAugust 1, 2017Banerjee K, Keasey MP, Razskazovskiy V, Visavadiya NP, Jia C, Hagg T.2 μg of template RNA with NEB's ProtoScript II kit according to manufacturer's instructions (Cat # E6560S, NEB). All PCR reactions were performed with a high fidelity Taq polymerase, using 2 μl of cDNA as a

(280) Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samplesNature ProtocolsJune 1, 2017Joshua Quick et al.Thermo Fisher Scientific, cat. no. N8080127) • Protoscript II First Strand cDNA Synthesis Kit (NEB, cat. no. E6560) • dNTP solution mix (NEB, cat. no. N0447) • Q5 Hot Start High-Fidelity DNA Polymerase (NEB, cat. no.

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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