ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(241) The Direct and Indirect Impact of SARS-CoV-2 Infections on Neonates: A Series of 26 Cases in BangladeshPediatric Infectious Disease JournalDecember 1, 2020Senjuti Saha et al.Quick-RNA/DNA Microprep Extraction Kit (D7005; Zymo), and was converted to cDNA using ProtoScript II (E6560; New England Biolabs, Ipswich, MA). Libraries prepared following the ARTIC pipeline,14 were sequenced

(242) High-resolution Repli-Seq defines the temporal choreography of initiation, elongation and termination of replication in mammalian cellsGenome BiologyDecember 1, 2020Peiyao A. Zhao, Takayo Sasaki, David M. GilbertFrom 0.5 μg total RNA, cDNA was synthesised by ProtoScript® II First Strand cDNA Synthesis Kit (NEB E6560) using dT23 primer. Ct was normalised against GAPDH. E/L Repli-seq library preparation and sequence

(243) The Direct and Indirect Impact of SARS-CoV-2 Infections on Neonates: A Series of 26 Cases in BangladeshPediatr Infect Dis JOctober 6, 2020Senjuti Saha, ASM Nawshad Uddin Ahmed, Probir Kumar Sarkar, Mohammed Rizwanul Ahsan Bipul, Kinkar Ghosh, Sheikh Wasik Rahman, Hafizur Rahman, Yogesh Hooda, Nafiz Ahsan, Roly Malaker, et al.Quick-RNA/DNA Microprep Extraction Kit (D7005; Zymo), and was converted to cDNA using ProtoScript II (E6560; New England Biolabs, Ipswich, MA). Libraries prepared following the ARTIC pipeline,14 were sequenced

(244) Neurodegeneration Caused by S1P-Lyase Deficiency Involves Calcium-Dependent Tau Pathology and Abnormal Histone AcetylationCellsSeptember 28, 2020Shah Alam, Antonia Piazzesi, Mariam Abd El Fatah, Maren Raucamp, Gerhild van Echten-Deckertthe ProtoScript® II First Strand cDNA Synthesis kit (New England Biolabs, Frankfurt/Main, Germany, E6560L). The resulting total cDNA was then applied to real-time PCR (CFX96-real time PCR, Bio-Rad Laboratories,

(245) Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine CellsSTAR protocolsSeptember 18, 2020Hor P, Ichida JK, Borok Z, Ryan AL.RNA Easy Mini Kit,Qiagen,"74004, 74134" Protoscript first strand synthesis kit,New England Biolabs,E6560S Enolase Quantikine ELISA Kit,R&D,DENL20 ,, Experimental Models: Cell Lines,Experimental Models: Cell

(246) Transcription of intragenic CpG islands influences spatiotemporal host gene pre-mRNA processingNucleic Acids ResearchSeptember 4, 2020Samuele M Amante et al.strand cDNA was synthesised using a ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560) using oligo d(T)23 primers and 500 ng total RNA. RT-qPCR RT-qPCR was performed on a QuantStudio 6

(247) Identification of Cardiac Glycosides as Novel Inhibitors of eIF4A1-Mediated Translation in Triple-Negative Breast Cancer CellsCancersAugust 4, 2020Howard CM, Estrada M, Terrero D, Tiwari AK, Raman D.synthesized using a ProtoScript® II First Strand cDNA Synthesis Kit (NEB, Ipswich, MA, USA, Cat. No. E6560S). Then, 1 μg of input RNA and oligo-dT primers were used according to the manufacturer’s instructions.

(248) Patterns of Herpes Simplex Virus 1 Infection in Neural Progenitor CellsJournal of VirologyJuly 30, 2020Wenxiao Zheng, Alissa M. Klammer, Jennifer N. Naciri, Jason Yeung, Matthew Demers, Jadranka Milosevic, Paul R. Kinchington, David C. Bloom, Vishwajit L. Nimgaonkar, Leonardo D’Aiutoperformed with random primer mix using a ProtoScript II ﬁrst-strand cDNA synthesis kit (catalog no. E6560S; New England BioLabs, Inc.) following the manufacturer’s instructions. The qPCR was performed using

(249) Heterogeneity in human hippocampal CaMKII transcripts reveals allosteric hub-dependent regulationScience SignalingJuly 23, 2020Roman Sloutsky, Noelle Dziedzic, Matthew J. Dunn, Rachel M. Bates, Ana P. Torres-Ocampo, Sivakumar Boopathy, Brendan Page, John G. Weeks, Luke H. Chao, Margaret M. Strattonfrom donor 1 and donor 2 RNA samples using the ProtoScript II first strand cDNA synthesis kit (NEB #E6560) with oligo dT priming. Variable regions of transcripts were PCR-amplified for 35 cycles with high-specificity

(250) Marine Oomycetes of the Genus Halophytophthora Harbor Viruses Related to BunyavirusesFrontiers in MicrobiologyJuly 15, 2020Leticia Botella, Josef Janoušek, Cristiana Maia, Marilia Horta Jung, Milica Raco, Thomas Jungand stored at −80°C. cDNA was synthesized using the ProtoScript II First Strand cDNA Synthesis Kit (E6560; New England Biolabs, MA, United States). Here, oligo d(T)23VN was incubated with the RNA at 65°C for

(251) Unbiased Proteomic Profiling Uncovers a Targetable GNAS/PKA/PP2A Axis in Small Cell Lung Cancer Stem CellsCancer cellJuly 13, 2020Coles GL, Cristea S, Webber JT, Levin RS, Moss SM, He A, Sangodkar J, Hwang YC, Arand J, Drainas AP, et al.Stranded Total RNA LT Kit,Illumina,Cat#15032612 ProtoScript cDNA Synthesis Kit,New England Biolabs,Cat#E6560L Simple Western Quantitative Immunoassay (Wes) 12-230kDa Plates,ProteinSimple,Cat#SM-W004 Wes anti-rabbit

(252) A molecular filter for the cnidarian stinging responseeLifeMay 26, 2020Weir K, Dupre C, van Giesen L, Lee AS, Bellono NW.TRIzol. RNA was isolated and DNase treated (Zymo Research), then used for cDNA library synthesis (NEB E6560). Full-length sequence for a Nematostella calcium channel beta subunit was obtained with a RACE strategy

(253) Rapid in-country sequencing of whole virus genomes to inform rabies elimination programmesWellcome Open ResearchMay 19, 2020Kirstyn Brunker, Gurdeep Jaswant, S.M. Thumbi, Kennedy Lushasi, Ahmed Lugelo, Anna M. Czupryna, Fred Ade, Gati Wambura, Veronicah Chuchu, Rachel Steenson, et al.31 Wellcome Open Research 2020, 5:3 Last updated: 09 NOV 2021 Protoscript II First Strand Sequencing kit (E6560, NEB) under manufacturer’s instructions. At later stages this was changed to Lunascript RT Supermix

(254) The HK5 and HK6 cytokinin receptors mediate diverse developmental pathways in riceDevelopmentApril 17, 2020Christian A. Burr et al.First-strand cDNA synthesis was performed with the ProtoScript II First Strand cDNA Synthesis Kit (NEB, E6560L) using poly-T primers. qRT-PCR was performed with PowerUp SYBR Green Master Mix (ThermoFisher, A25742)

(255) Epigenetic control of the EWS‑FLI1 promoter in Ewing''s sarcomaOncology reportsApril 1, 2020Montoya C, Rey L, Rodríguez J, Fernández MJ, Troncoso D, Cañas A, Moreno O, Henríquez B, Rojas A.reverse transcription of 2 µg RNA using the ProtoScript® II First Strand cDNA Synthesis kit (cat. no. E6560S; New England BioLabs, Inc.). PCR reactions were performed with Taq DNA Polymerase products (cat. no.

(256) LRG1 May Accelerate the Progression of ccRCC via the TGF-β PathwayBioMed Research InternationalMarch 30, 2020Quan Hong, Shuqiang Wang, Shuxin Liu, Xiangmei Chen, Guangyan Caia discovery cohort. 2.4. qPCR Detection The First Strand cDNA Synthesis Kit (New England Biolabs, E6560S) was used for cDNA synthesis with a standard protocol. Each RNA/d(T)23VN sample was predenatured for

(257) A Dimeric Structural Scaffold for PRC2-PCL Targeting to CpG Island ChromatinMolecular cellMarch 19, 2020Chen S, Jiao L, Liu X, Yang X, Liu X.PfuUltra High-Fidelity DNA Polymerase,Agilent Technologies,Cat# 600385 ProtoScript® II First Strand cDNA Synthesis Kit,NEB,Cat# E6560S Bio-Rad gel filtration standard,BioRad,cat # 1511901 Yeast tRNA (10 mg/mL),Invitrogen,cat # AM7119 Amersham Hybond-N

(258) Analysis of Global Collection of Group A Streptococcus Genomes Reveals that the Majority Encode a Trio of M and M-Like ProteinsmSphereFebruary 26, 2020Hannah R. Frost et al.was estimated using a QuickDrop spectrophotometer (Molecular Devices). Reverse transcriptase (NEB; E6560) was performed using approximately 300 ng of RNA and a 6 ␮M concentration of the random primer mix provided

(259) Cardiac injury modulates critical components of prostaglandin E₂ signaling during zebrafish heart regenerationScientific reportsFebruary 20, 2020FitzSimons M, Beauchemin M, Smith AM, Stroh EG, Kelpsch DJ, Lamb MC, Tootle TL, Yin VP.cDNA was synthesized using ProtoScript® II First Strand cDNA Kit (New England Biolabs, Ipswich, MA # E6560S). qPCR studies were performed in technical triplicate using Brilliant III Ultra-Fast SYBR® Green QPCR Master Mix

(260) Loss of Imprinting in Human Placentas Is Widespread, Coordinated, and Predicts Birth PhenotypesMolecular Biology and EvolutionFebruary 1, 2020Claudius Vincenz, Jennie L Lovett, Weisheng Wu, Kerby Shedden, Beverly I Strassmannperformed immediately after DNaseI digestion of RNA with the ProtoScriptII First Strand cDNA Synthesis Kit (E6560, New England Biolabs) and random hexamer primers. Pyrosequencing primers were designed with Qiagen PyroMark

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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