ProtoScript II First Strand cDNA Synthesis Kit E6560L from New England Biolabs

Product Specs
ItemProtoScript II First Strand cDNA Synthesis Kit
CompanyNew England Biolabs
Price $737.00 Inquire
Catalog NumberE6560L
Quantity150 reactions
ApplicationsDNA Analysis
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(221) Drosophila STING protein has a role in lipid metabolismeLifeSeptember 1, 2021Akhmetova K, Balasov M, Chesnokov I.generated from 1 μg of total RNA using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560). RT-qPCR analysis was performed in Luna Universal qPCR Master Mix (New England Biolabs, #M3003) using

(222) Targeted RNA-seq improves efficiency, resolution, and accuracy of allele specific expression for human term placentasG3 Genes|Genomes|GeneticsAugust 7, 2021Weisheng Wu, Jennie L Lovett, Kerby Shedden, Beverly I Strassmann, Claudius Vincenzimmediately after DNaseI digestion of placental RNA with the ProtoScript®II First Strand cDNA Synthesis Kit (E6560, New England Biolabs) and random hexamer primers. Qiagen PyroMark Assay Design 2.0 software was used

(223) Baculovirus actin-rearrangement-inducing factor ARIF-1 induces the formation of dynamic invadosome clustersMolecular Biology of the CellAugust 1, 2021Domokos I. Lauko, Taro Ohkawa, Sergio E. Mares, Matthew D. Welchreverse-transcribed to cDNA using a Protoscript II First Strand DNA Synthesis kit (New England Biolabs; E6560S) using random hexamers as primers. S. frugiperda cortactin-specific primers were used to PCR-amplify

(224) Baculovirus actin-rearrangement-inducing factor ARIF-1 induces the formation of dynamic invadosome clustersMol Biol CellAugust 1, 2021Domokos I. Lauko, Taro Ohkawa, Sergio E. Mares, Matthew D. Welchreverse-transcribed to cDNA using a Protoscript II First Strand DNA Synthesis kit (New England Biolabs; E6560S) using random hexamers as primers. S. frugiperda cortactin-specific primers were used to PCR-amplify

(225) Structure and analysis of nanobody binding to the human ASIC1a ion channeleLifeJuly 28, 2021Wu Y, Chen Z, Sigworth FJ, Canessa CM.200521,Mutagenesis of DNA Commercial assay or kit,ProtoScrript II First strand cDNA,New England Biolabs,Cat#: E6560L,Synthesis of single strand DNA "Chemical compound, drug",Pierce anti-HA magnetic beads,Thermo Fisher Scientific

(226) Reciprocal priming between receptor tyrosine kinases at recycling endosomes orchestrates cellular signalling outputsThe EMBO journalJuly 15, 2021Smith MP, Ferguson HR, Ferguson J, Zindy E, Kowalczyk KM, Kedward T, Bates C, Parsons J, Watson J, Chandler S, et al.tests),Cambridge Bioscience,11‐1100 ProtoScript; II First Strand cDNA Synthesis Kit,New England Biolabs,E6560L ReliaPrep RNA Cell Miniprep System,Promega,Z6011 Tumor dissociation kit,Miltenyi Biotec,130‐095‐929 Isolate II PCR and Gel kit

(227) Orphan CpG islands amplify poised enhancer regulatory activity and determine target gene responsivenessNature geneticsJuly 1, 2021Pachano T, Sánchez-Gaya V, Ealo T, Mariner-Faulí M, Bleckwehl T, Asenjo HG, Respuela P, Cruz-Molina S, Muñoz-San Martín M, Haro E, et al.845-KS-2040250). cDNA was generated using ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs, E6560L). RT-qPCRs were performed on the Light Cycler 480II (Roche) using Eef1a1 and Hptr as housekeeping genes.

(228) Persistent NF-κB activation in muscle stem cells induces proliferation-independent telomere shorteningCell reportsMay 11, 2021Tichy ED, Ma N, Sidibe D, Loro E, Kocan J, Chen DZ, Khurana TS, Hasty P, Mourkioti F.Assay,Sekisui Diagnostics,Cat# 326-10 Protoscript II cDNA First Strand Synthesis Kit,New England Biolabs,Cat# E6560S Qiafilter Plasmid Midi Kit,QIAGEN,Cat# 12243 Rneasy Plus Micro Kit,QIAGEN,Cat# 74034 Trichrome Staining

(229) Emergence and rapid transmission of SARS-CoV-2 B.1.1.7 in the United StatesCellMay 1, 2021Nicole L. Washington, Karthik Gangavarapu, Mark Zeller, Alexandre Bolze, Elizabeth T. Cirulli, Kelly M. Schiabor Barrett, Brendan B. Larsen, Catelyn Anderson, Simon White, Tyler Cassens, et al.al., 2017) and reagents from the Protoscript First Strand cDNA Synthesis Kit (New England Biolabs, #E6560L) and the Q5 Hot Start High-Fidelity DNA Polymerase Kit (New England Biolabs, #M0493L). Amplicons were

(230) Control of Neuronal Excitability by Cell Surface Receptor Density and Phosphoinositide MetabolismFrontiers in PharmacologyApril 21, 2021Martin Kruse, Rayne J. Whittenreverse transcription using the ProtoScript II First Strand cDNA Synthesis Kit (New England Biolabs Cat# E6560S) with D(T)23 VN oligonucleotides based on manufacturer instructions. Subsequently, synthesized cDNA

(231) Repeated introductions and intensive community transmission fueled a mumps virus outbreak in Washington StateElifeApril 19, 2021Louise H Moncla, Allison Black, Chas DeBolt, Misty Lang, Nicholas R Graff, Ailyn C Pérez-Osorio, Nicola F Müller, Dirk Haselow, Scott Lindquist, Trevor Bedfordassay or kit,Protoscript II First strand synthesis kit,"New England Biolabs, Ipswich MD, USA",Cat #: E6560L, Commercial assay or kit,Q5 Hotstart DNA polymerase,"New England Biolabs, Ipswich, MD, USA",Cat #:

(232) Repeated introductions and intensive community transmission fueled a mumps virus outbreak in Washington StateeLifeApril 19, 2021Louise H Moncla, Allison Black, Chase DeBolt, Misty Lang, Nicholas R Graff, Ailyn C Pérez-Osorio, Nicola F Müller, Dirk Haselow, Scott Lindquist, Trevor Bedfordassay or kit,Protoscript II First strand synthesis kit,"New England Biolabs, Ipswich MD, USA",Cat #: E6560L, Commercial assay or kit,Q5 Hotstart DNA polymerase,"New England Biolabs, Ipswich, MD, USA",Cat #:

(233) Live-cell imaging reveals kinetic determinants of quality control triggered by ribosome stallingMolecular cellApril 15, 2021Goldman DH, Livingston NM, Movsik J, Wu B, Green R.manufacturer protocol. RNA was reverse-transcribed using ProtoScript II First Strand cDNA Synthesis Kit (NEB, #E6560), according to manufacturer protocol, using the random primer mix and 4 ul RNA per 20 ul reaction. A

(234) Genome-wide binding potential and regulatory activity of the glucocorticoid receptor's monomeric and dimeric formsNature communicationsMarch 31, 2021Johnson TA, Paakinaho V, Kim S, Hager GL, Presman DM.per the manufacturer’s instructions. cDNA reactions were performed with the NEB Protoscript II kit (#E6560S) per the manufacturer’s instructions. QPCRs were performed with iQ SYBR Green Supermix (Biorad # 1708880)

(235) Interplay of BAF and MLL4 promotes cell type-specific enhancer activationNature communicationsMarch 12, 2021Park YK, Lee JE, Yan Z, McKernan K, O'Haren T, Wang W, Peng W, Ge K.(Life Technologies) and reverse transcribed using ProtoScript II first-strand cDNA synthesis kit (NEB, E6560), following manufacturer’s instructions. Quantitative RT-PCR (qRT-PCR) was performed in duplicate with

(236) Postembryonic screen for mutations affecting spine development in zebrafishDevelopmental BiologyMarch 1, 2021Ryan S. Gray, Roberto Gonzalez, Sarah D. Ackerman, Ryoko Minowa, Johanna F. Griest, Melisa N. Bayrak, Benjamin Troutwine, Stephen Canter, Kelly R. Monk, Diane S. Sepich, et al.Cloning and Genotyping cDNA was synthesized using the ProtoScriptII First Strand cDNA synthesis kit (NEB E6560S). Cloning of the full length admats9 from pooled cDNA from 3, 5, and 15 dpf AB*zebrafish, due to issues

(237) Protocol for Proximity-Dependent Proteomic Profiling in Yeast Cells by APEX and Alk-Ph ProbeSTAR ProtocolsDecember 30, 2020Yi Li, Keke Liu, Ying Zhou, Jing Yang, Peng ZouClean & Concentrator-100 Kit,Zymo,Cat# R1019 ProtoScript® II First Strand cDNA Synthesis Kit,NEB,Cat# E6560 ,, Experimental Models: Organisms/Strains,Experimental Models: Organisms/Strains,Experimental Models:

(238) Decoding an organ regeneration switch by dissecting cardiac regeneration enhancersDevelopment (Cambridge, England)December 23, 2020Begeman IJ, Shin K, Osorio-Méndez D, Kurth A, Lee N, Chamberlain TJ, Pelegri FJ, Kang J.synthesized from 300 ng to 1 μg of total RNA using a NEB ProtoScript II first strand cDNA synthesis kit (NEB, E6560). To design lepb qPCR primers for other Danio species, we initially ran a PCR on gDNA of each species

(239) SARS-CoV-2 Transmission among Marine Recruits during QuarantineNew England Journal of MedicineDecember 17, 2020Andrew G. Letizia, Irene Ramos, Ajay Obla, Carl Goforth, Dawn L. Weir, Yongchao Ge, Marcas M. Bamman, Jayeeta Dutta, Ethan Ellis, Luis Estrella, et al.of RNA was used as input for a cDNA reaction with the NEB Protoscript II First Strand Synthesis Kit (E6560) using random hexamer primers, according to the manufacturer’s instructions. The Thermo Fisher Scientific

(240) Mitochondrial dysfunction induces RNA interference in C. elegans through a pathway homologous to the mammalian RIG-I antiviral responsePLOS BiologyDecember 2, 2020Kai Mao, Peter Breen, Gary RuvkunTRIzol. RT-qPCR The cDNA was generated by ProtoScript II First Strand cDNA Synthesis Kit (New England BioLabs, #E6560L). qPCR was performed toward eol-1, nduo-5, and ctb-1 with act-1 as control by iQ SYBR Green Supermix

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ProtoScript II First Strand cDNA Synthesis Kit

ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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ProtoScript II First Strand cDNA Synthesis Kit from New England Biolabs

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