ROS/Superoxide Detection Assay Kit (Cell-based) ab139476 from Abcam

Product Specs
ItemROS/Superoxide Detection Assay Kit (Cell-based)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139476
Quantity1 Kit
Sample TypeAdherent cells, Suspension cells
Assay Time0h 90m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(61) Ligand-induced gene activation is associated with oxidative genome damage whose repair is required for transcriptionProceedings of the National Academy of Sciences of the United States of AmericaSeptember 8, 2020Sengupta S, Wang H, Yang C, Szczesny B, Hegde ML, Mitra S.cellular O2.– and H2O2 levels were measured by using the Superoxide Detection Assay Kit (Abcam; no. ab139476) and Hydrogen Peroxide Assay Kit (Cell Biolabs; no. STA-844), respectively, per the manufacturers’ instructions. Subcellular

(62) Marriage of black phosphorus and Cu(2+) as effective photothermal agents for PET-guided combination cancer therapyNature communicationsJune 8, 2020Hu K, Xie L, Zhang Y, Hanyu M, Yang Z, Nagatsu K, Suzuki H, Ouyang J, Ji X, Wei J, et al.according to the manufacturer’s protocol for the cellular ROS/superoxide detection assay kit (Abcam, ab139476). Briefly, before treatment, the medium was replaced with fresh medium. Then, either vehicle or BPNS

(63) 4-Hexylresorcinol and silk sericin increase the expression of vascular endothelial growth factor via different pathwaysScientific ReportsDecember 1, 2019You-Young Jo, Dae-Won Kim, Je-Yong Choi, Seong-Gon KimROS and superoxide levels were measured using a cellular ROS/Superoxide detection assay kit (CAT#: ab139476, Abcam). The procedure was performed in accordance with the manufacturer’ protocol. After incubating

(64) The fungal metabolite chaetocin is a sensitizer for pro-apoptotic therapies in glioblastomaCell Death & DiseaseDecember 1, 2019Ezgi Ozyerli-Goknar et al.buffer and centrifuged at RT. Cells were induced by loading with ROS/Superoxide detection mix (Abcam, ab139476 kit) supplemented with above mentioned treatments and incubated for 30 min in the cell culture incubator

(65) Closing the Mitochondrial Permeability Transition Pore in hiPSC-Derived Endothelial Cells Induces Glycocalyx Formation and Functional MaturationStem cell reportsNovember 12, 2019Tiemeier GL, Wang G, Dumas SJ, Sol WMPJ, Avramut MC, Karakach T, Orlova VV, van den Berg CW, Mummery CL, Carmeliet P, et al.intracellular reactive oxygen species, we used the Cellular ROS/Superoxide detection assay kit (Abcam, ab139476). ECs plated in a black 96-well plate (20,000 cells per well) were incubated with ROS/Superoxide detection

(66) IL-37 Attenuates Lung Fibrosis by Inducing Autophagy and Regulating TGF-β1 Production in MiceThe Journal of ImmunologyOctober 15, 2019Mi So Kim, Ae Rin Baek, June Hyuk Lee, An Soo Jang, Do Jin Kim, Su Sie Chin, Sung Woo ParkCalbiochem-Novabiochem (La Jolla, CA). Reactive oxygen species (ROS)/superoxide detection assay kits (no. ab139476; Abcam) and lipid peroxidation assay kits (no. 118970; Abcam) were used. The in situ cell death detection

(67) Pseudomonas aeruginosa Quorum Sensing Molecule Alters Skeletal Muscle Protein Homeostasis by Perturbing the Antioxidant Defense SystemmBioOctober 1, 2019Bandyopadhaya A, Tzika AA, Rahme LG.levels in myotubes were measured using the Cellular ROS/Superoxide Detection assay kit (catalog no. ab139476; Abcam). The C2C12 myotube culture conditions and treatments were the same as described above. Following

(68) miR-425 deficiency promotes necroptosis and dopaminergic neurodegeneration in Parkinson’s diseaseCell Death & DiseaseAugust 1, 2019Yong-Bo Hu, Yong-Fang Zhang, Hao Wang, Ru-Jing Ren, Hai-Lun Cui, Wan-Ying Huang, Qi Cheng, Hong-Zhuan Chen, Gang Wangoxidative stress production was investigated through the Cellular ROS/Superoxide detection assay kit (ab139476, Abcam, UK) following the manufacturer’s protocol. Briefly, cells were seeded onto 96-well black/clear

(69) Reduced Basal Nitric Oxide Production Induces Precancerous Mammary Lesions via ERBB2 and TGFβScientific reportsApril 30, 2019Ren G, Zheng X, Bommarito M, Metzger S, Walia Y, Letson J, Schroering A, Kalinoski A, Weaver D, Figy C, et al.lines of the MCF10A progression series was determined with Cellular ROS/Superoxide Detection Assay Kit (ab139476, Abcam), which measures total reactive oxygen species and superoxide separately, according to the manufacturer’s

(70) An In Vitro Investigation of Cytotoxic Effects of InP/Zns Quantum Dots with Different Surface ChemistriesNanomaterials (Basel)January 22, 2019Deanna Ayupova, Garima Dobhal, Geoffry Laufersky, Thomas Nann, Renee V. Gorehamcombination of two specific fluorescent probes. Cellular ROS/Superoxide detection assay kit (Abcam, ab139476) was chosen to provide the real-time measurement of global levels of ROS, as well as superoxide concentrations

(71) Effect of Cyclic Stretch on Vascular Endothelial Cells and Abdominal Aortic Aneurysm (AAA): Role in the Inflammatory ResponseInternational Journal of Molecular SciencesJanuary 12, 2019Martina Ramella et al.Cellular ROS/Superoxide Detection Assay Kit Oxidative stress production was investigated through Cellular ROS/Superoxide detection assay kit (ab139476, Abcam, Cambridge, UK) following the manufacturer’s protocol. Briefly, detached cells (static controls,

(72) Tumor-Stroma Mechanics Coordinate Amino Acid Availability to Sustain Tumor Growth and MalignancyCell metabolismJanuary 8, 2019Bertero T, Oldham WM, Grasset EM, Bourget I, Boulter E, Pisano S, Hofman P, Bellvert F, Meneguzzi G, Bulavin DV, et al.Bioscience,CAK1065 NAD/NADH quantification kit,Sigma-Aldrich,MAK037 Cellular ROS/Superoxide detection assay kit,Abcam,ab139476 Glutamate dehydrogenase activity assay kit,Abcam,ab102527 GSH/GSSG Ratio detection assay kit,Abcam,ab138881 LysylOxidase

(73) Nitrosative stress uncovers potent β₂-adrenergic receptor-linked vasodilation further enhanced by blockade of clathrin endosome formationAmerican Journal of Physiology-Heart and Circulatory PhysiologyJune 1, 2018Mary D. Frame, Anthony M. Dewar, Rhodora C. Calizo, Androniqi Qifti, Suzanne F. ScarlataZeiss LSM software, as previously described (3, 14). ROS kit experiments. We used a commercial kit (ab139476, Abcam) to determine changes in the level of reactive oxygen species (ROS). This kit included the oxidative

(74) Aerobic exercise regulates blood lipid and insulin resistance via the toll‑like receptor 4‑mediated extracellular signal‑regulated kinases/AMP‑activated protein kinases signaling pathwayMolecular Medicine ReportsApril 11, 2018Mei Wang, Sen Li, Fubaihui Wang, Jinhui Zou, Yanfeng Zhang(1:500 dilution; cat no. ab13533), CAT (1:500 dilution; cat no. ab52477), ROS (1:500 dilution; cat no. ab139476), ERK (1:500 dilution; cat no. ab17942), AMPK (1:500 dilution; cat no. ab32047), and β‑actin (1:500

(75) Mitotic defects lead to neuronal aneuploidy and apoptosis in frontotemporal lobar degeneration caused by MAPT mutationsMolecular Biology of the CellMarch 1, 2018Julbert Caneus, Antoneta Granic, Rosa Rademakers, Dennis W. Dickson, Christina M. Coughlan, Heidi J. Chial, Huntington Potterwithout Nutlin-3 (Selleckchem; Cat. No. S1061). A cellular ROS/Superoxide Detection Assay Kit (Abcam; ab139476) was used to measure oxidative stress levels in cultured cells. FISH analysis Single nuclei suspensions

(76) A Map of Human Mitochondrial Protein Interactions Linked to Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and NF-κB SignalingCell systemsDecember 27, 2017Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, Goebels F, Musso G, Wu Z, et al.Commercial Assays,Critical Commercial Assays Cellular ROS/superoxide anion detection kits,Abcam,Cat# ab139476 Phospho-NF-κB p65 (Ser536) (93H1) mAb,Cell Signaling,Cat# 3033 E.Z.N.A. gel extraction,Omega Bio,Cat#

(77) Biallelic mutations in the ferredoxin reductase gene cause novel mitochondriopathy with optic atrophyHuman Molecular GeneticsDecember 15, 2017Yanyan Peng et al.measured ROS generation levels in fibroblasts with the Cellular ROS/Superoxide Detection Assay Kit (ab139476, Abcam; Cambridge, UK). Seeded cells were placed directly onto glass slides or polystyrene tissue culture

(78) 18α-Glycyrrhetinic acid lethality for neuroblastoma cells via de-regulating the Beclin-1/Bcl-2 complex and inducing apoptosisBiochemical pharmacologyOctober 1, 2016Rahman MA, Bishayee K, Habib K, Sadra A, Huh SO.

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8/21/2023ROS Detection Assay Kit From Abcam Shivali RawatIn my study, proteostasis stress resulted in the aggregation of mitochondrial proteins inside cells. To investigate the effect on mitochondrial function, I experimented to test levels of ROS under ... Read Review

ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

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ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

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