ROS/Superoxide Detection Assay Kit (Cell-based) ab139476 from Abcam

Product Specs
ItemROS/Superoxide Detection Assay Kit (Cell-based)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139476
Quantity1 Kit
Sample TypeAdherent cells, Suspension cells
Assay Time0h 90m

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Website: http://www.abcam.com

(41) Ginsenoside Rg1 ameliorates apoptosis, senescence and oxidative stress in ox‑LDL‑induced vascular endothelial cells via the AMPK/SIRT3/p53 signaling pathwayExperimental and Therapeutic MedicineJune 30, 2022Tian-Jiao Lyu, Zi-Xiu Zhang, Jun Chen, Zong-Jun Liuspecies (ROS), malondialdehyde (MDA) and superoxide dismutase (SOD) levels The levels of ROS (cat. no. ab139476; Abcam), MDA (cat. no. A003-1-2; Nanjing Jiancheng Bioengineering Institute) and SOD (cat. no. A001-3-2;

(42) The Molecular Role of HIF1α Is Elucidated in Chronic Myeloid LeukemiaFrontiers in OncologyJune 30, 2022Vivek Singh, Ranjana Singh, Rashmi Kushwaha, Shailendra Prasad Verma, Anil Kumar Tripathi, Abbas Ali Mahdi(51), superoxide dismutase (52), lipid peroxidation (53), and protein carbonyl (54). ROS/SOD by Abcam-ab139476 kit, Nitric oxide assay kit (colorimetric)- ab65328, protein carbonyl content assay kit- ab126287, and

(43) Melanin nanoparticles enhance the neuroprotection of mesenchymal stem cells against hypoxic‐ischemic injury by inhibiting apoptosis and upregulating antioxidant defenseCell Biology InternationalJune 1, 2022Chunliu Tang et al.of ROS and superoxide were detected by using the intracellular ROS/superoxide detection assay kit (ab139476; Abcam). Neurons cultivated in coverslips at a density of 1 × 106 cells per well (12‐well plates) after

(44) The bioengineered HALOA complex induces anoikis in chronic myeloid leukemia cells by targeting the BCR-ABL/Notch/Ikaros/Redox/Inflammation axisJ Med LifeMay 1, 2022Vivek Singh, Ranjana Singh, Abbas Ali Mahdi, Anil Kumar Tripathianalyzed with 2% agarose gel electrophoresis at 100 V for 60 minutes [13]. Redox Assay ROS/SOD by Abcam-ab139476 kit, Nitric oxide assay kit (colorimetric)-ab65328, protein carbonyl content assay kit-ab126287, Antioxidant

(45) Oxaliplatin induces ferroptosis and oxidative stress in HT29 colorectal cancer cells by inhibiting the Nrf2 signaling pathwayExperimental and Therapeutic MedicineApril 13, 2022Ben Liu, Hui WangScientific, Inc.). The levels of ROS in HT29 cells were measured with a commercially available kit (cat. no. ab139476, Abcam) according to the manufacturers' instructions. Briefly, the control cells were pretreated with

(46) Oxidative Stress- and Autophagy-Inducing Effects of PSI-LHCI from Botryococcus braunii in Breast Cancer CellsBioTech (Basel)March 31, 2022Freisa M. Joaquín-Ovalle et al.(version 5.20). 2.8. ROS/Superoxide Production Assay The ROS/Superoxide Detection Assay Kit (Abcam ab139476) was used to detect total ROS and superoxide production in live MDA-MB-231 cells. First, cells were

(47) Inhibition of Autophagy Facilitates XY03-EA-Mediated Neuroprotection against the Cerebral Ischemia/Reperfusion Injury in RatsOxidative Medicine and Cellular LongevityMarch 30, 2022Wenwen Cui, Yuanyuan Hao, Mingye Wang, Qiuyan Zhang, Junmei Wang, Gang Wei, Yunlong HouSuperoxide Assessments ROS and superoxide levels in the live cells were measured using a commercial kit (ab139476, Abcam, USA). The procedure was performed in accordance with the manufacturer' protocol. Upon staining,

(48) Omega-3 fatty acids impair miR-1-3p-dependent Notch3 down-regulation and alleviate sepsis-induced intestinal injuryMolecular medicine (Cambridge, Mass.)January 28, 2022Chen YL, Xie YJ, Liu ZM, Chen WB, Zhang R, Ye HX, Wang W, Liu XY, Chen HS.2′,7′-dichlorofluorescein diacetate staining method with the help of ROS/Superoxide Detection Assay kits (Cell-based) (ab139476, Abcam). Firstly, the homogenate of colon tissues was extracted from the mice and diluted to a ratio

(49) Repurposing Ceritinib Induces DNA Damage and Enhances PARP Inhibitor Responses in High-Grade Serous Ovarian CarcinomaCancer researchJanuary 15, 2022Kanakkanthara A, Hou X, Ekstrom TL, Zanfagnin V, Huehls AM, Kelly RL, Ding H, Larson MC, Vasmatzis G, Oberg AL, et al.hours. ROS and peroxide levels were measured using a cell-based fluorogenic assay kit (catalog no. ab139476, Abcam). Quantitative real-time PCR Total RNA was extracted from cells using an miRNeasy mini kit

(50) Vitamin D has an effect on airway inflammation and Th17/Treg balance in asthmatic miceThe Kaohsiung journal of medical sciencesDecember 1, 2021Ma JG, Wu GJ, Xiao HL, Xiao YM, Zha L.Massachusetts). The level of reactive oxygen species (ROS) was detected using the ROS/Superoxide kit (ab139476, Abcam). The content of superoxide dismutase (SOD) was detected using the SOD kit (ab65354, Abcam).

(51) FABP4 knockdown suppresses inflammation, apoptosis and extracellular matrix degradation in IL-1β-induced chondrocytes by activating PPARγ to regulate the NF-κB signaling pathwayMolecular Medicine ReportsOctober 14, 2021Huajie Mao, Bin Han, Hao Li, Yiqing Tao, Weigang Wucontent in cells was detected by using a ROS/Superoxide Detection Assay kit (Cell-based) (cat. no. ab139476; Abcam). The expression of superoxide dismutase (SOD) was detected using a SOD kit (cat. no. ab277415;

(52) Endothelial Aryl Hydrocarbon Receptor Nuclear Translocator Mediates the Angiogenic Response to Peripheral Ischemia in Mice With Type 2 Diabetes MellitusFrontiers in Cell and Developmental BiologyJune 10, 2021Tu Nguyen et al.media for 48 h, the ROS levels were assessed using the Cellular ROS/Superoxide Detection Assay Kit (ab139476 from Abcam), which has been widely and successfully utilized in many different studies (Hu et al., 2019,

(53) Astragaloside IV Protects 6-Hydroxydopamine-Induced SH-SY5Y Cell Model of Parkinson’s Disease via Activating the JAK2/STAT3 PathwayFrontiers in NeuroscienceMarch 23, 2021ZhengHu Xu, Dongfeng Yang, Xiaojing Huang, Huai HuangMA, United States). The level of reactive oxygen species (ROS) was detected using the ROS/Superoxide kit (ab139476, Abcam). The content of superoxide dismutase (SOD) was detected using the SOD kit (ab65354, Abcam). Flow Cytometry

(54) KLF11 protects against abdominal aortic aneurysm through inhibition of endothelial cell dysfunctionJCI insightMarch 8, 2021Zhao G, Chang Z, Zhao Y, Guo Y, Lu H, Liang W, Rom O, Wang H, Sun J, Zhu T, et al.superoxide and ROS in HAECs was measured using the Cellular ROS/Superoxide Detection Assay Kit (Abcam, ab139476) following the manufacturer’s protocol. Briefly, HAECs were infected with Ad-shKLF11 or Ad-KLF11 or

(55) Pulsed electromagnetic fields synergize with graphene to enhance dental pulp stem cell-derived neurogenesis by selectively targeting TRPC1 channelsEuropean cells & materialsMarch 1, 2021Madanagopal TT, Tai YK, Lim SH, Fong CH, Cao T, Rosa V, Franco-Obregón A.production were quantified using a cellular ROS/Superoxide Detection Assay Kit (cell-based) (Abcam, ab139476). hDPSCs were seeded onto either 2DG or glass and neurogenic differentiation was induced using neurogenic induction

(56) Resveratrol protects against high glucose‑induced oxidative damage in human lens epithelial cells by activating autophagyExperimental and Therapeutic MedicineFebruary 26, 2021Pengzhi Chen, Zhenyu Yao, Zhihui HeHLECs were measured by flow cytometry using the Cell-based ROS/Superoxide Detection Assay kit (cat. no. ab139476; Abcam) according to the manufacturer's instructions. Briefly, HLECs after specified treatments were

(57) Beneficial Effect of Tempol, a Membrane-Permeable Radical Scavenger, on Inflammation and Osteoarthritis in In Vitro ModelsBiomoleculesFebruary 25, 2021Giovanna Calabrese, Alessio Ardizzone, Michela Campolo, Sabrina Conoci, Emanuela Esposito, Irene Paternitiintracellular reactive oxygen species, we used the Cellular ROS/Superoxide detection assay kit (Abcam, ab139476, Cambridge, MA, USA) [29]. CC cells plated in a black 96-well plate (20,000 cells per well) were incubated

(58) Protective Effect of Epigallocatechin-3-Gallate in Hydrogen Peroxide-Induced Oxidative Damage in Chicken LymphocytesOxidative Medicine and Cellular LongevityDecember 31, 2020Xiaoqing Chi, Xiaodan Ma, Zoushuyi Li, Yong Zhang, Yong Wang, Lijia Yuan, Ye Wu, Wei Xu, Songhua Hu(Rockville, MD, USA); assay kits for reactive oxygen species (ROS) and superoxide dismutase (SOD) (ab139476), genomic DNA extraction kit (ab156900), and intracellular calcium ion ([Ca2+]i) detection kit (Fluo3/AM)

(59) Enterovirus 71 induces neural cell apoptosis and autophagy through promoting ACOX1 downregulation and ROS generationVirulenceDecember 1, 2020You L, Chen J, Liu W, Xiang Q, Luo Z, Wang W, Xu W, Wu K, Zhang Q, Liu Y, et al.FITC Annexin V Apoptosis Detection Kit was purchased from BD Biosciences. Cellular ROS detection assay kit (ab139476) was purchased from Abcam. Lentivirus production and infection Oligonucleotides target human ACOX1

(60) Inhibition of Lysosomal Function Mitigates Protective Mitophagy and Augments Ceramide Nanoliposome-Induced Cell Death in Head and Neck Squamous Cell CarcinomaMolecular cancer therapeuticsDecember 1, 2020Shaw JJP, Boyer TL, Venner E, Beck PJ, Slamowitz T, Caste T, Hickman A, Raymond MH, Costa-Pinheiro P, Jameson MJ, et al.Assay: Cells were treated as described in the text and the ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476) (Abcam) and Luminescent ATP Detection Assay Kit (ab113849) (Abcam) were utilized according to the manufacturer’s

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8/21/2023ROS Detection Assay Kit From Abcam Shivali RawatIn my study, proteostasis stress resulted in the aggregation of mitochondrial proteins inside cells. To investigate the effect on mitochondrial function, I experimented to test levels of ROS under ... Read Review

ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

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ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

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