ROS/Superoxide Detection Assay Kit (Cell-based) ab139476 from Abcam

Product Specs
ItemROS/Superoxide Detection Assay Kit (Cell-based)
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab139476
Quantity1 Kit
Sample TypeAdherent cells, Suspension cells
Assay Time0h 90m

Add to Compare List

Abcam

Discovery Drive

Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

Company Profile

Website: http://www.abcam.com

(21) In vitro toxicity of Lithium bis(trifluoromethanesulfonyl)imide (LiTFSI) on Human Renal and Hepatoma CellsToxicol RepMarch 1, 2024Xing Zhang, Mia Sands, Mindy Lin, Jennifer Guelfo, Joseph Irudayarajsuperoxide were measured in A498, and HepG2 cells by ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476; Abcam; Waltham, MA, USA). Cells were loaded with the ROS/Superoxide Detection Mix at 37°C for 30–60

(22) Luteolin prevents TNF-α-induced NF-κB activation and ROS production in cultured human placental explants and endothelial cellsPlacentaJanuary 1, 2024Adrian C. Eddy, Augustine Rajakumar, Frank T. Spradley, Joey P. Granger, Sarosh Ranawere seeded on 4-well chamber slides as above. The ROS/superoxide detection assay kit (Abcam; Cat # ab139476) was used with the associated protocol. Briefly, cells were pre-treated with luteolin (5μM) for 1 hour

(23) Andrographolide nanophytosomes exhibit enhanced cellular delivery and pro-apoptotic activities in HepG2 liver cancer cellsDrug deliveryDecember 1, 2023Neamatallah T, Malebari AM, Alamoudi AJ, Nazreen S, Alam MM, Bin-Melaih HH, Abuzinadah OA, Badr-Eldin SM, Alhassani G, Makki L, et al.Reactive oxygen species determination Reactive oxygen species (ROS)/Superoxide detection assay kit (ab139476, Abcam, Cambridge, MA, USA) was used to assess intracellular reactive oxygen species following the manufacturer’s

(24) Oxathiazinane derivatives display both antineoplastic and antibacterial activity: a structure activity studyJournal of Cancer Research and Clinical OncologySeptember 1, 2023B. Majchrzak-Stiller, M. Buchholz, I. Peters, J. Strotmann, J. Möhrke, L. Zelichowski, L. Oehlke, C. Quensel, D. Fein, P. Höhn, et al.level of the cells. Measurements were performed using the Cellular ROS/Superoxide Detection Assay KIT (ab139476, Abcam, Cambridge, UK) following the manufacturer’s instructions. Measurements were performed at 490

(25) CXCL8 Promotes Endothelial-to-Mesenchymal Transition of Endothelial Cells and Protects Cells from Erastin-Induced Ferroptosis via CXCR2-Mediated Activation of the NF-κB Signaling PathwayPharmaceuticals (Basel)August 25, 2023Hai-zhou Ji, Li Chen, Mi Ren, Sang Li, Tong-yu Liu, Hong-ju Chen, Hui-hui Yu, Yang SunGSH/GSSG Ratio Detection Assay (S0053, Beyotime), ROS/Superoxide Detection Assay Kit (Cell-based) (ab139476, Abcam), Lipid Peroxidation (MDA) Assay Kit (S0131S, Beyotime), and CCK-8 Kit (Beyotime). 4.9. Detection

(26) GP‐2250, a novel anticancer agent, inhibits the energy metabolism, activates AMP‐Kinase and impairs the NF‐kB pathway in pancreatic cancer cellsJournal of Cellular and Molecular MedicineJuly 1, 2023Britta Majchrzak‐Stiller, Marie Buchholz, Ilka Peters, Daniel Waschestjuk, Johanna Strotmann, Philipp Höhn, Stephan Hahn, Chris Braumann, Waldemar Uhl, Thomas Müller, et al.passages. 2.11 ROS assay ROS was analysed using the Cellular ROS/Superoxide Detection Assay Kit (ab139476, Abcam) 21 following the manufacturer's instructions. 2.12 NF‐κB transcription factor binding assay The

(27) Grpel2 maintains cardiomyocyte survival in diabetic cardiomyopathy through DLST-mediated mitochondrial dysfunction: a proof-of-concept studyJournal of translational medicineMarch 16, 2023Yang R, Zhang X, Zhang Y, Wang Y, Li M, Meng Y, Wang J, Wen X, Yu J, Chang P.software (NIH, USA). Total cellular ROS in NCMs were detected by a ROS/Superoxide Detection Assay Kit (Ab139476, Abcam, USA) as previously described via a microplate reader [22]. The mitochondrial ROS in NCMs were

(28) Metal Nanoparticles with Antimicrobial Properties: The Toxicity Response in Mouse Mesenchymal Stem CellsToxicsMarch 9, 2023Pavel Rossner, Tereza Cervena, Barbora Echalar, Katerina Palacka, Alena Milcova, Zuzana Novakova, Michal Sima, Zuzana Simova, Jolana Vankova, Vladimir HolanPeroxidation Intracellular ROS production was measured using a ROS/Superoxide Detection assay kit (ab139476, Abcam, Cambridge, UK) according to the manufacturer’s instructions. Briefly, MSC grown in 96 well black

(29) Arthrocolins Synergizing with Fluconazole Inhibit Fluconazole-Resistant Candida albicans by Increasing Riboflavin Metabolism and Causing Mitochondrial Dysfunction and AutophagyMicrobiology spectrumFebruary 27, 2023Wu Z, Wu QF, Yuan WL, Chen YH, Hu D, Deng DY, Zhang LL, Niu XM.over time. Reactive oxygen species assay. ROS/superoxide detection assay kit (Abcam; catalog no. ab139476) was used to monitor production of reactive oxygen species (ROS). The fungal conidia were stained with

(30) Characterization of Oxygen Nanobubbles and In Vitro Evaluation of Retinal Cells in HypoxiaTranslational Vision Science & TechnologyFebruary 10, 2023Victoria Messerschmidt, Wen Ren, Michael Tsipursky, Joseph Irudayarajdocument from Kerafast. ROS and Superoxide Determination The ROS and Superoxide Detection assay kit (ab139476, Abcam, Waltham, MA) was used to determine the level of ROS and superoxide after ONBs treatment following

(31) Tryptophan-centered metabolic alterations coincides with lipid-mediated fungal response to cold stressHeliyonFebruary 1, 2023Yonghong Chen, Xiaoyu Yang, Longlong Zhang, Qunfu Wu, Shuhong Li, Jianghui Gou, Jiangbo He, Keqin Zhang, Shenghong Li, Xuemei Niusoftware (BD Biosciences, USA) [8]. 5.12 ROS/superoxide assay ROS/Superoxide Detection Assay Kit (#ab139476, Abcam) was used to directly monitor real time production of reactive oxygen species (ROS) in mycelia

(32) Altered ethanol metabolism and increased oxidative stress enhance alcohol‐associated liver injury in farnesoid X receptor‐deficient miceLiver InternationalJanuary 1, 2023Caroline Morel, Vivek Chowdhary, Prashanth Thevkar Thevkar Nagesh, Marcelle Ribeiro, Danielle Hawryluk, Donna Catalano, Luciano Adorini, Gyongyi SzaboAssay Reactive oxygen species (ROS) levels were detected using the manufacturers’ protocol (Abcam, ab139476). Briefly, bone marrow-derived macrophages (BMDMs) (50 000 cells) or hepatocytes (25 000 cells) were

(33) Kurarinone alleviates hemin-induced neuroinflammation and microglia-mediated neurotoxicity by shifting microglial M1/M2 polarization via regulating the IGF1/PI3K/Akt signalingThe Kaohsiung journal of medical sciencesDecember 1, 2022Jia ZQ, Zuo C, Yue WF.microglia/SH‐SY5Y cells, SH‐SY5Y cells were lysed and centrifuged for assay of the content or activity of ROS (ab139476; Abcam), malondialdehyde (MDA; ab118970; Abcam), superoxide dismutase (SOD; ab65354; Abcam), and catalase

(34) Myoglobin-mediated lipid shuttling increases adrenergic activation of brown and white adipocyte metabolism and is as a marker of thermogenic adipocytes in humansClinical and translational medicineDecember 1, 2022Christen L, Broghammer H, Rapöhn I, Möhlis K, Strehlau C, Ribas-Latre A, Gebhardt C, Roth L, Krause K, Landgraf K, et al.brown adipocytes, pre‐treated with or without 1 μM CL for 60 min, were measured using a standard assay kit (ab139476, Abcam). 4.2.7 Oxygen consumption assays 3T3‐L1, imBA or primary brown adipocytes were seeded into

(35) Nox4 expression in osteo-progenitors controls bone development in mice during early lifeCommunications BiologyDecember 1, 2022Jin-Ran Chen, Oxana P. Lazarenko, Michael L. Blackburn, Jennifer F. Chen, Christopher E. Randolph, Jovanny Zabaleta, Katrin Schroder, Kim B. Pedersen, Martin J. J. Ronispreviously38. Measurement of ROS Cell-based ROS/superoxide detection assay kit was purchased from Abcam (abcam.com), cat#ab139476. According to manufactory’s recommendation, this assay Kit was designed to directly monitor real-time

(36) Parkin regulates adiposity by coordinating mitophagy with mitochondrial biogenesis in white adipocytesNature communicationsNovember 4, 2022Moore TM, Cheng L, Wolf DM, Ngo J, Segawa M, Zhu X, Strumwasser AR, Cao Y, Clifford BL, Ma A, et al.detection Hydrogen peroxide and superoxide were detected using ROS/superoxide detection assay kit (Abcam, ab139476). Briefly, 3T3-L1 adipocytes were cultured in 96 well plate and incubated in ROS/Superoxide detection

(37) LINC02389/miR-7-5p Regulated Cisplatin Resistance of Non-Small-Cell Lung Cancer via Promoting Oxidative StressAnalytical Cellular PathologyOctober 19, 2022Peng Ma, Wen Han, Cunying Meng, Xiaohong Tan, Pengfei Liu, Lei Dongthe miR-7-5p expression. 2.9. ROS The ROS/superoxide detection assay kit (cell-based) (Abcam, UK, ab139476) was utilized to detect the ROS level in each group. Seed the cells into polystyrene tissue culture

(38) Biologically Synthesized Rosa rugosa-Based Gold Nanoparticles Suppress Skin Inflammatory Responses via MAPK and NF-ÎºB Signaling Pathway in TNF-Î±/IFN-Î³-Induced HaCaT KeratinocytesACS OmegaOctober 11, 2022Rongbo Wang, Sung-Kwon Moon, Woo-Jung Kim, Sanjeevram Dhandapani, Hoon Kim, Yeon-Ju KimROS Detection After T + I stimulation for 24 h, ROS was detected using an ROS detection assay kit (ab139476; Abcam, Cambridge, UK) and a fluorescence microscope (Leica Microsystems) according to the manufacturer’s

(39) Therapeutic Potential of Mesenchymal Stem Cell-Secreted Factors on Delay in Corneal Wound Healing by Nitrogen MustardInternational journal of molecular sciencesSeptember 29, 2022An S, Shen X, Anwar K, Ashraf M, Lee H, Koganti R, Ghassemi M, Djalilian AR.and basal media for 24 h. After 24 h, cells were treated with ROS/Superoxide Detection Mix reagent (#ab139476, Abcam, Cambridge, Massachusetts, UK) in a culture medium for 30 min. For confocal microscopy, cells

(40) Mitochondrial mitophagy protection combining rivaroxaban and aspirin in high glucose-exposed human coronary artery endothelial cell. An in vitro studyDiabetes & vascular disease researchSeptember 1, 2022Zekri-Nechar K, Zamorano-León JJ, Cortina-Gredilla M, López-de-Andrés A, Jiménez-García R, Navarro-Cuellar C, López-Farré A, Martínez-Martínez CH.was determined by a fluorescence (fluorescein Ex/Em = 490/525 nm) microplate assay commercial kit (ab139476, Abcam, Cambridge, UK) following the manufacturer´s instructions. ROS production was measured after

Write a Review

(1)

8/21/2023ROS Detection Assay Kit From Abcam Shivali RawatIn my study, proteostasis stress resulted in the aggregation of mitochondrial proteins inside cells. To investigate the effect on mitochondrial function, I experimented to test levels of ROS under ... Read Review

ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

Abcam

Citations (78)

Reviews

ROS/Superoxide Detection Assay Kit (Cell-based) from Abcam

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map