Nano-Glo Luciferase Assay N1120 from Promega

Product Specs
ItemNano-Glo Luciferase Assay
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberN1120
Quantity100ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutationsMolecular Therapy OncologyJune 18, 2026Joshua Sammons, Jianguo Chen, Kari Thrasher, Lianwu Fu, Ming Du, Hui Wen, J. Robert Bostwick, Paige Vinson, Omar Moukha-Chafiq, Corinne Augelli-Szafran, et al.well was used to perform the NanoLuc assay with the Nano-Glo luciferase assay system (Promega, Cat#N1120) using the GloMax Discover System (Promega). The lysate protein concentration was measured with the

(2) A transferable SARS-CoV-2 IRES module enables dual translation initiation for enhanced antigen expression in COVID-19 mRNA vaccinesMol Ther Nucleic AcidsJune 16, 2026Han Young Seo, Haewon Jung, Se-Young Lee, Hae-Gwang Jung, Hee Cho, Yu-Min Son, Yeonju Bak, Seo-Yeon Hwang, Jung-Hee Kim, In Ho Park, et al.and immunoblotting. NanoLuc luciferase activity was measured using the Nano-Glo Luciferase Assay System (Promega; cat. no. N1120) according to the manufacturer’s protocol. Briefly, harvested cell lysates were clarified by centrifugation

(3) Nucleoprotein and glycoprotein based serological assays for detection of Marburg virus infectionseBioMedicineMay 1, 2026James Kagame, Markus H. Kainulainen, Emmanuel Kabalisa, Polina Brangel, Jean Marie Vianney Uwimana, Jessica R. Harmon, Nouh Saad Mohamed, Jean Luc Benimana, Ange Umwari, Elif Karaaslan, et al.incubated for 20 min at room temperature before adding 30 μl of NanoGlo substrate (Promega, Cat. no. N1120). The plate was read 10 min later using a GloMax® Navigator microplate reader (Promega). Luminescence

(4) Disruption of spike protein N-glycosylation induces its endoplasmic reticulum retention and attenuates SARS-CoV-2 infectivityJournal of VirologyApril 21, 2026Weili Kong, Jiali Zhang, Yingying Song, Jingjing Song, Yuebo Xu, Xinmu Xu, Haoyu Ma, Li Chen, Cong ZengBuffer (Cat# 21004) was purchased from Thermo Scientific. The Nano-Glo Luciferase Assay System (Cat# N1120) was purchased from Promega. The SuperKine Maximum Sensitivity Cell Counting Kit-8 (Cat# BMU106-CN)

(5) STARR-CRAAVT: A platform to identify cell type-specific regulatory elements for next-generation gene therapyiScienceApril 17, 2026Robert Becker, Priyanka Choudhury, Martin Oti, Youssef Fouani, Benjamin Strobel, Stephanie Ketterer, Simon Rumpel, John Park, Sebastian Kreuz, Udo Maier, et al.Index Primer Pairs,New England Biolabs,Cat. #E6440L Nano-Glo® Luciferase Assay System,Promega,Cat. #N1120 ,, Deposited data,Deposited data,Deposited data ,, HCC ATAC-Seq peaks,Genomic Data Commons (GDC)/Corces

(6) 2'-O-Methylation maintains ribosome structural and translation integrityMol CellApril 16, 2026Yu Zhao, Jay Rai, Lauren N. Cohen, Yuerong Shu, Chong Xu, Hemant Goswami, Xin Chen, Hong Jin, Virginie Marchand, Yuri Motorin, et al.AG,NEB,E2080S Monarch® Spin RNA Cleanup Kit,NEB,T2050L The Nano-Glo® Luciferase Assay System,Promega,N1120 Yeast plasmid miniprep system,Zymo Research,D2001 Click-iT™ HPG Alexa Fluor™ 488 Protein Synthesis Assay Kit

(7) Human antibodies against West Nile and related orthoflavivirusesbioRxivApril 6, 2026Tomás Cervantes Cervantes Rincón, Tereza Frckova, Zaria I. Contejean, Jasmine Cantergiani, Kevin Groen, Benedetta Cena, Simone G. Moro, Filippo Bianchini, Luca Simonelli, David Jarrossay, et al.PBS, and 50 μL lysis buffer added (Promega, E1531). The Nano-Glo Luciferase Assay System (Promega, N1120) with GloMAx Discover System reader (Promega) were used to measure the NanoLuc luciferase activity of

(8) Targeting oncogenic NTSR1 with liensinine reprograms Gq-mediated signaling to suppress lung adenocarcinomaChin MedMarch 25, 2026Yongfu Wang, Wei Liu, Pengzhuo Tao, Changmin Liu, Yizhen Yuan, Yajing Xue, Hanting Yang, Xiongfeng Liu, Xinyi Zhou, Shilin Chen, et al.37 °C for 30 min. Under light-protected conditions, Nano-Glo® Luciferase Assay substrate (Promega, #N1120) was added, and after a 5-min equilibration at room temperature, BRET signals were quantified using

(9) One-step knock-in CAR constructs in human NK cells enable scalable, TGFβ1-resistant immunotherapy for solid tumorsTheranosticsMarch 25, 2026Su-Min Yee, Ji Hye Jeong, Daeun Kim, Sung-Bae Kang, Hanna Yoon, Hyein Cho, Daechan Park, Eunsung Jun, Mihue Jang(89901, Thermo Fisher Scientific). The resulting lysates were mixed with Nano-Glo Luciferase substrate (N1120; Promega, Madison, WI, USA), and the luminescence intensity was measured using a GloMax Microplate Reader

(10) Enhancing cancer killing and natural killer cell persistence by targeting NOXA, a predictor of poor patient survivalMolecular Therapy OncologyMarch 19, 2026Sung-Bae Kang, Ji Hye Jeong, Seung Wook Kim, Hyejin Yoo, Seunghun Lee, Ji-Hye Oh, Chang Ohk Sung, Seung-Hwan Lee, Eunsung Jun, Mihue JangIL-15 (200-15-250UG; PeproTech). Luminescence was measured using the Nano-Glo Luciferase Assay System (N1120; Promega, Madison, WI, USA) per the manufacturer’s instructions. Luminescence intensity was used as

(11) A comparative analysis of CD70-directed CAR-T cells for glioblastoma treatment demonstrates a superior efficacy of the ligand-based constructMolecular Therapy OncologyMarch 19, 2026Alexandros Kourtesakis, Hiu Nam Hannah Chow, Eileen Bailey, Sandra Horschitz, Ammar Jabali, Rainer Will, Christoph Schifflers, Abigail K. Suwala, Hannah Rohdjess, Melissa Hahn, et al.line. The functionality of the NLuc gene was verified using the Nano-Glo Luciferase Assay System kit (#N1120, Promega) according to the manufacturer’s instructions. Generation of cerebral organoids Human induced pluripotent stem cells

(12) The Fatty Acid Transporter CD36 Mediates Uptake, Biodistribution, and Cardioprotection by Small Extracellular Vesicles From HEK293 CellsJournal of Extracellular VesiclesMarch 17, 2026Elias Sulaiman, Derek M. Yellon, Sean M. DavidsonLuciferase Assay HEK293‐sEVs were detected in vitro by using the Nano‐Glo Luciferase Assay System (N1120, Promega) as previously described (Sulaiman et al. 2025). 10 µL of SEC fractions or 1 µL of HEK293 cell

(13) Immune cells regulate circulating adipocyte extracellular vesicle levels in response to metabolic shiftsCell MetabolismMarch 10, 2026Snigdha Tiash, Saket Awadhesbhai Patel, Marjori Russo, Anusha Suresh, Bo Pang, Yun-Ling Pai, Wentong Jia, Rachael L. Field, Terri Pietka, Nada A. Abumrad, et al.commercial assays,Critical commercial assays Promega Nano-Glo™ Luciferase Assay System,Promega,Cat# N1120 Mouse Adiponectin ELISA Kit,Millipore Sigma,Cat# EZMADP Mix-n-Stain™ CF® Dye Antibody Labeling Kit

(14) GPR39 mediated molecular signaling by bile acidsJournal of Biological ChemistryFebruary 4, 2026Yanyun Huang, Zhentao Zi, Chenliang Xia, Yi Raowith HBSS and incubated with 80 μl of HBSS per well containing the Nano-Glo substrate (Promega, cat# N1120, diluted 1:200) for 2 min. Luminescence was measured at 460 ± 40 nm and 535 ± 15 nm using a BioTek Cytation5

(15) Regulation of virion production by the ORF8 signal peptide across SARS-CoV-2 variantsbioRxivFebruary 3, 2026Mir M. Khalid, Irene P. Chen, Frank S. Soveg, Taha Y. Taha, Takako Tabata, Rahul K. Suryawanshi, Abdullah M. Syed, Alison Ciling, Maria McCavitt-Malvido, Ursula Schulze-Gahmen, et al.medium was replaced and 72 hours post-infection 50μl of sup was used for nano-luciferase assay (Promega, N1120) according to manufacturer protocol. Luminescence was measured using a TECAN plate reader (Spark) with

(16) Identification of novel small chemical compounds that inhibit Ebola virus VP40-mediated virus-like particle productionVirus ResearchFebruary 1, 2026Tomoko Tsuruta, Satoshi Mizuta, Ayako Izumisawa, Jiro Yasuda, Shuzo Uratacells, and both were lysed using the Nano-Glo Assay Reagent (Nano-Glo Luciferase assay system, Promega, N1120). After incubation for 10–30 min at room temperature, each sample was transferred to a white 96-well

(17) Expanding the toolbox to develop IAP-based degraders of TEAD transcription factorsCommunications chemistryJanuary 19, 2026Gupta N, Trainor N, Radwan M, Nguyen S, Duncan L, Tang AX, Beveridge J, Silke N, Yousef J, Bilgilier C, et al.incubated at 37 °C, 10% CO2 for 1 h. A 10 μL NanoGlo Substrate (1/50 dilution in Opti-MEM, Promega, N1120) was transferred per well and BRET signals were collected on a CLARIOstarPlus plate reader (BMG Labtech)

(18) Targeting the ERα DBD-LBD Interface with Mitoxantrone Disrupts Receptor Function through Proteasomal DegradationMolecular cancer therapeuticsJanuary 2, 2026Wang H, Luo Y, Artham S, Wang Q, Peng Y, Yun Z, Li X, Wu C, Liu Z, Weber-Bonk KL, et al.a 3:1 lipid:DNA ratio. After 48 hours, 25 μL of 5X Nano-Glo Luciferase assay substrate (Promega, # N1120) was added per well in Nano-Glo LCS dilution buffer (Promega, #N1150). Following a 5-minute room temperature

(19) HTRF-based identification of small molecules targeting SARS-CoV-2 E protein interaction with ZO-1 PDZ2Scientific reportsDecember 28, 2025Alvarez F, Larrous F, Mechaly A, Bardiaux B, Goor Q, Haouz A, Séon-Méniel B, de Sousa RA, Bourg S, Desmaële D, et al.for 72 h post-infection, after which luminescence was measured following the manufacturer’s protocol (N1120, Promega). Briefly, 10 µL of cell culture supernatant was diluted 10-fold into a final reaction volume

(20) Developing Synthetic Full-Length SARS-CoV-2 cDNAs and Reporter Viruses for High-Throughput Antiviral Drug ScreeningVirusesDecember 27, 2025Megha Rohamare, Nidhi Kaushik, Juveriya Qamar Khan, Mahrokh Balouchi, Joaquin Lopez-Orozco, Robert Kozak, Tom C. Hobman, Darryl Falzarano, Anil Kumar, Joyce A. WilsonNluc expression was measured using the Nano-Glo® luciferase assay system (Promega, Madison, WI, USA, #N1120) after equilibrating the 96-well plate at room temperature for 10 min. Then, 50 μL volume of the Nano-Glo® luciferase assay

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3/16/2017NanoLuciferase Assay System Nina FlerinOur laboratory investigates different ways to improve immune responses against HIV.. We developed different mouse models to study the efficacy of therapeutic candidates against HIV. Some of these mice... Read Review
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3/2/2017Nano-Glo® Luciferase Assay System Review Lana JagannathanOur lab uses HIV molecular clones that express Nano luciferase reporter. This reporter is very stable and allows quatification of low HIV infection, because it provides a very sensitive reading. We ... Read Review

Nano-Glo Luciferase Assay

Nano-Glo Luciferase Assay from Promega

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Nano-Glo Luciferase Assay from Promega

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