ROS-ID® Total ROS detection kit ENZ-51011 from Enzo Life Sciences, Inc.

Product Specs
ItemROS-ID® Total ROS detection kit
CompanyEnzo Life Sciences, Inc.
Price Supplier Page View Company Product Page
Catalog NumberENZ-51011
ApplicationsFlow Cytometry, Fluorescence microscopy, Fluorescent detection, HTS
Quantity1 Kit
Detection TargetROS

Specifications/Features

Directly monitors global levels of reactive oxygen species (ROS), but not superoxide in live cells
Distinguishes between different reactive species, such as hydrogen peroxide, peroxynitrite and hydroxyl radicals when specific inhibitors are used
High sensitivity, specificity and accuracy for live cell studies
Compatible with major components of tissue culture media (phenol red, FBS and BSA)
Complete set of reagents, including ROS inducers and scavengers
Stringently manufactured, to control and eliminate non-specific assay artifacts

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Enzo Life Sciences, Inc.

10 Executive Blvd.

Farmingdale, NY 11735

United States

Phone: 800-942-0430
Fax: 610-941-9252

Company Profile

Website: http://www.enzolifesciences.com

(1) Source-Specific Photobiomodulation Regulates Mitochondrial Bioenergetics, Redox Signaling, and Functional Outputs in C2C12 Myoblasts Across Replicative AgingInternational Journal of Molecular SciencesMarch 25, 2026Ana Elena Aviña, Nguyen Le Thanh Hang, Che-Yi Chang, Yi-Fan Chen, Yun Yen, Xavier Pei-Chun Wong, Aline Yen Ling Wang, Cheng-Jen Chang, Tzu-Sen Yangassessed using the ROS-ID® Total ROS Detection Kit (Enzo Life Sciences, Inc., Farmingdale, NY, USA; Cat# ENZ-51011). Cells were seeded at 1 × 105 cells per well in 24-well plates and allowed to adhere overnight. Following

(2) Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 CellInternational Journal of Molecular SciencesFebruary 22, 2025Min Jung Kim, Ye Jin Yang, Ji Woong Heo, Jae-dong Son, Young Zoo You, Ju-Hye Yang, Kwang Il ParkAfterward, ROS stained using ROS-ID total ROS detection kit according to the manufacturer’s protocol (ENZ-51011, Enzo Life Sciences, Plym, MA). DCF-DA (Thermo Fisher Scientific, Waltham, MA, USA) was introduced and

(3) Impact of singular versus combinatorial environmental stress on RONS generation in Drosophila melanogaster larvaeFrontiers in PhysiologyAugust 29, 2024Pratibha Bomble, Bimalendu B. NathRONS concentration Total RONS generation was quantified using the ROS-ID® Total ROS Detection Kit (ENZ-51011, Enzo Chemicals) in the larval hemolymph of D. melanogaster from both the control and treated groups,

(4) Hypoxia‐induced oxidative stress promotes therapy resistance via upregulation of heme oxygenase‐1 in multiple myelomaCancer MedicineApril 1, 2023Ko Abe, Sho Ikeda, Miho Nara, Akihiro Kitadate, Hiroyuki Tagawa, Naoto Takahashispecies detection on flow cytometry The reactive oxygen species (ROS)‐ID Total ROS Detection Kit (ENZ‐51011) was purchased from Enzo Life Sciences. ROS detection was performed using FACSCanto or FACSLyric (BD Biosciences

(5) A Selective SARS-CoV-2 Host-Directed Antiviral Targeting Stress Response to Reactive Oxygen SpeciesACS central scienceJanuary 25, 2023Tang C, Coelho AR, Rebelo M, Kiely-Collins H, Carvalho T, Bernardes GJL.level of reactive oxygen species (ROS) was measured using the ROS-ID Total ROS detection kit (Enzo, Cat#ENZ-51011), according to the manufacturer’s instructions. Briefly, VERO-CCL 81 cells were seeded in 96-well black

(6) Differential manifestation of RONS and antioxidant enzymes in response to singular versus combinatorial stress in Chironomus ramosusStress BiologyDecember 29, 2022Pratibha Bomble, Bimalendu B. Nathconcentration Total RONS generation was quantified by using total RONS detection kit (Enzo chemical, ENZ-51011) in salivary gland cells of C. ramosus larvae of control and treatment group by following manufacture’s

(7) Suppression of Ribose-5-Phosphate Isomerase a Induces ROS to Activate Autophagy, Apoptosis, and Cellular Senescence in Lung CancerInternational Journal of Molecular SciencesJuly 17, 2022Yu-Chin Nieh, Yu-Ting Chou, Yu-Ting Chou, Chao-Yung Wang, Shi-Xian Lin, Shih-Ci Ciou, Chiou-Hwa Yuh, Horng-Dar Wanganalysis of ROS cells was performed by FACS (BD). The detection reagent was performed by ENZO (Catalog No: ENZ-51011). For the cells prepared for analysis, the cells were first washed twice with 1X ROS buffer, then resuspended

(8) Molecular and Cellular Response of the Myocardium (H9C2 Cells) Towards Hypoxia and HIF-1α InhibitionFrontiers in cardiovascular medicineJanuary 1, 2022Osuru HP, Lavallee M, Thiele RH.ROS levels were measured using a 2′-7′-dichlorofluorescein diacetate-based total ROS detection kit (#ENZ-51011, Enzo Life Sciences). Oxidative stress levels were assayed using Protein Carbonyl assay using the bioluminescent assay

(9) Age-dependent instability of mature neuronal fate in induced neurons from Alzheimer's patientsCell stem cellSeptember 2, 2021Mertens J, Herdy JR, Traxler L, Schafer ST, Schlachetzki JCM, Böhnke L, Reid DA, Lee H, Zangwill D, Fernandes DP, et al.Click-iT EdU Alexa Fluor 488 Imaging Kit,Thermo Fisher Scientific,Cat#C10337 Total ROS detection kit,Enzo Life Sciences,Cat#ENZ-51011 ProteinSimple 12-230 kDa Separation Module,Bio-Techne,Cat#SM-W004 ProteinSimple Jess 25-Capillary

(10) TET2 as a tumor suppressor and therapeutic target in T-cell acute lymphoblastic leukemiaProceedings of the National Academy of Sciences of the United States of AmericaAugust 24, 2021Bensberg M, Rundquist O, Selimović A, Lagerwall C, Benson M, Gustafsson M, Vogt H, Lentini A, Nestor CE.were treated with 100 μL of the desired concentration of vitamin C in ROS detection solution (Enzo, ENZ-51011) according to the manufacturer’s instructions. After 10 min of treatment, ROS was detected by measuring

(11) Toll-like receptor 4 is activated by platinum and contributes to cisplatin-induced ototoxicityEMBO reportsMay 5, 2021Babolmorad G, Latif A, Domingo IK, Pollock NM, Delyea C, Rieger AM, Allison WT, Bhavsar AP.response of cisplatin treatment in vitro using the Total ROS‐ID detection kit (Enzo Life Sciences, ENZ‐51011). 5 × 103 HEI‐OC1, Tlr4‐deleted HEI‐OC1, or control cells were seeded in 96‐well plates. Cell density

(12) TMBIM6 (transmembrane BAX inhibitor motif containing 6) enhances autophagy through regulation of lysosomal calciumAutophagyMarch 1, 2021Kim HK, Lee GH, Bhattarai KR, Lee MS, Back SH, Kim HR, Chae HJ.protocol. ROS measurement For ROS detection, the ROS-ID Total ROS detection kit (Enzo Life Sciences, ENZ-51011) was used according to the manufacturer’s instructions. Real-time PCR analysis Total RNA was isolated

(13) Stress Response of Mouse Embryonic Fibroblasts Exposed to Polystyrene NanoplasticsInternational journal of molecular sciencesFebruary 20, 2021Han SW, Choi J, Ryu KY.assay was performed using an ROS-ID® Total ROS detection kit following the manufacturer’s protocol (#ENZ-51011, Enzo Life Sciences, Farmingdale, NY, USA). Briefly, cells were washed with PBS and ROS detection solution

(14) Angiotensin II increases activity of the ClC-K2 Cl(-) channel in collecting duct intercalated cells by stimulating production of reactive oxygen speciesThe Journal of biological chemistryJanuary 1, 2021Khayyat NH, Zaika O, Tomilin VN, Pyrshev K, Pochynyuk O.split-opened collecting ducts were loaded with the oxidative stress detection reagent (Enzo Life Sciences, ENZ-51011) in 1X Wash Buffer for 45 min at room temperature according to the manufacturer’s protocol. Fluorescent

(15) The Impaired Bioenergetics of Diabetic Cardiac Microvascular Endothelial CellsFrontiers in endocrinologyJanuary 1, 2021Zhang H, Shen Y, Kim IM, Weintraub NL, Tang Y.ROS Detection in Endothelial Cells ROS was measured using the total ROS-ID Total ROS Detection Kit (ENZ-51011, Enzo Life Sciences, Farmingdale, NY) according to the manufacturer’s instructions. Brieﬂy, CMEC from

(16) β-Glucan-Induced Trained Immunity in DogsFrontiers in ImmunologyOctober 9, 2020Simon Paris et al.peroxide, peroxynitrite and hydroxyl radicals was achieved using the ROS-ID® Total ROS detection kit (ENZ-51011, ENZO Life Sciences, Inc.). All experiments were performed following the manufacturer's recommendations.

(17) Probiotic Properties of Escherichia coli Nissle in Human Intestinal OrganoidsmBioAugust 25, 2020Suman Pradhan, Alison Ann Weissproduction, infected HIOs were injected with 100 nl of 300 pM ROS detection reagent (catalog number ENZ-51011; Enzo Life Sciences) at the times indicated above. The HIOs were further incubated at 37°C with 5% CO2

(18) Neutralization of Oxidized Phospholipids Ameliorates Non-alcoholic SteatohepatitisCell metabolismJanuary 7, 2020Sun X, Seidman JS, Zhao P, Troutman TD, Spann NJ, Que X, Zhou F, Liao Z, Pasillas M, Yang X, et al.Mitochondrial membrane potential measurement: Total ROS was determined with Total ROS detection Kit (ENZ-51011 Enzo Life Sciences Inc.) and mitochondrial ROS levels using MitoSOX (Invitrogen) following the manufacturers’

(19) BETP degradation simultaneously targets acute myelogenous leukemia stem cells and the microenvironmentThe Journal of clinical investigationFebruary 21, 2019Piya S, Mu H, Bhattacharya S, Lorenzi PL, Davis RE, McQueen T, Ruvolo V, Baran N, Wang Z, Qian Y, et al.experiments with standards. Measurement of total ROS. Total ROS levels of AML cells were measured using an ENZ-51011 Kit (Enzo Life Sciences) according to the manufacturer’s protocol. Briefly, 5 × 105 cells were pelleted and

(20) PUMILIO hyperactivity drives premature aging of Norad-deficient miceeLifeFebruary 8, 2019Florian Kopp et al.line. Analysis of reactive oxygen species (ROS) and oxidative damage The Enzo Total ROS Detection Kit (ENZ-51011, Enzo Life Sciences) was used for detection of ROS levels in immortalized MEFs and human HCT116 cells.

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(1)

10/5/2020Detection of Total ROS Level in Primary Microglia Joanna PlastiraPrimary murine microglia cells were treated with LPA in the presence or absence of different MAPK inhibitors and the total ROS levels were detected after different time periods.
(1)

6/19/2015Total ROS detection in eosinophils Daniela JanevskaI work on trying to determine the source of ROS production in eosinophils following the engagement of a eosinophil specific surface receptor. I used this kit to understand the kinetics of ROS ... Read Review

ROS-ID® Total ROS detection kit from Enzo Life Sciences, Inc.

Description

Specifications/Features

Enzo Life Sciences, Inc.

Citations (28)

Reviews

ROS-ID® Total ROS detection kit from Enzo Life Sciences, Inc.

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