Enzo
ROS-ID® Total ROS detection kit
ENZ-51011
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Primary murine microglia cells were treated with LPA in the presence or absence of different MAPK inhibitors and the total ROS levels were detected after different time periods.
Total ROS assay
Primary murine microglia
Perform multiple experiments, there is increased variation between replicates.
Inhibition of MAPK signalling decreased the LPA induced production of ROS.
https://doi.org/10.1186/s12974-020-01809-1
Easy and fast to use.
High variation between replicates. The reconstituted detection reagent has a shelf life of just 1 week.
An easy to use kit as long as you optimize it.