Pyruvate Assay Kit ab65342 from Abcam

Product Specs
ItemPyruvate Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65342
Quantity100 Test
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay Time0h 40m
SensitivityMore than 1 µM
Detection Range1 - 200 µM
Research AreaMetabolism

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) Esketamine Attenuates Postoperative Neurocognitive Disorder in Aged Mice: Associations with Bioenergetic Remodeling in Hippocampal CD11b-Enriched Cell Fractions and Glycolysis-Related SignalingDrug Des Devel TherAugust 25, 2026Di Liu, Guangchun Li, Hui Huang, Yong Yang, Fei Jiang, Yue Zhangmeasured using Abcam assay kits (L-Lactate Assay Kit, Cat. No. ab65330; Pyruvate Assay Kit, Cat. No. ab65342; Pyruvate Kinase Assay Kit, Cat. No. ab83432; and LDH Assay Kit, Cat. No. ab102526; Abcam, Cambridge,

(2) PDK4 drives abdominal aortic aneurysm by promoting smooth muscle cell metabolic reprogramming and NLRP3-mediated pyroptosisNature CommunicationsApril 11, 2026Li Zhao, Xuefeng Lin, Zhengqiang Zhu, Ranxin Liu, Lingna Zhao, Xuekun Wu, Mengru Zheng, Rihua Huang, Pengyu Zhou, Fangze Huang, et al.according to the manufacturer’s protocol. Pyruvate levels were measured using the Pyruvate Assay Kit (ab65342, Abcam) according to the manufacturer’s protocol. Statistical analysis All statistical analyses were

(3) Metabolic Reprogramming of T Cells by Dual UCP2 and IL‐17 Blockade Enhances Immunity Against Pancreatic CancerAdvanced ScienceMarch 1, 2026Chuan‐Teng Liu, Chun‐Chieh Yeh, Tsai‐Chen Wu, Chia‐Hsin Lin, Yi‐Ting Kuo, Yoichiro Iwakura, Yuan‐Ji Day, Charles Drake, Vedran Radojcic, Ying‐Chyi Song, et al.quantification. Mitochondrial pyruvate and acetyl‐CoA levels were measured using the Pyruvate Assay Kit (ab65342, Abcam) and PicoProbe Acetyl‐CoA Assay Kit (ab87546, Abcam) according to the manufacturer's protocols. 4.9

(4) Damage-induced pyroptosis drives endogenous thymic regeneration by activating the purinergic receptor P2Y2Cell Death & DiseaseJanuary 3, 2026Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Erin Kirsche, Makya Warren, Paul deRoos, Antonella Cardinale, Lorenzo Iovino, David Granadier, Colton W. Smith, et al.intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega, J5021). Thymocytes were isolated from untreated and TBI-treated mice

(5) SYK regulates Schwann cell metabolic reprogramming to promote axonal regeneration in immune-mediated neuropathyJournal of NeuroinflammationDecember 6, 2025Nannan Zhang, Chen Liu, Fangyu Chen, Qian Zheng, Yuzhong Wang, Fangzhen Shanlevels were quantified using kits: glucose-6-phosphate (Abcam, Cat#ab176722), pyruvate (Abcam, Cat#ab65342), lactate (Abcam, Cat#ab65331). Absorbance was read at 450 nm for glucose-6-phosphate) or 570 nm for

(6) Lactate as a metabolic–epigenetic signal linking high-intensity interval training (HIIT) to miRNA-Centered remodeling of the skeletal muscle methylome and transcriptomeRedox BiologyDecember 1, 2025Lei Zhou, Soroosh Mozaffaritabar, Kumpei Tanisawa, Takuji Kawamura, Mitsuru Higuchi, Istvan Boldogh, Xueqing Ba, Sataro Goto, George Brooks, Yaodong Gu, et al.concentrations in gastrocnemius muscle were measured using assay kits (Abcam; lactate kit ab65330, pyruvate kit ab65342, αKG kit ab83431, NAD/NADH kit ab176723). Approximately 20 ± 2 mg of homogenized tissue from the pre-mixed

(7) SKA3-mediated hypoxia tolerance and metabolic reprogramming promote liver metastasis in lung adenocarcinomaCell Death & DiseaseNovember 26, 2025Zedong Sun, Minmin Zhou, Ningdan Song, Can Wang, Mingrui Liu, Simian He, Feifan Ji, Jiayu Chen, Lin Xu, Xiufen Zheng, et al.secretion, and pyruvate production was performed using kits from Abcam (catalog nos. ab136955, ab65330, and ab65342) according to the manufacturer’s instructions. Cell counting was performed using a cell counter. Seahorse

(8) Focal adhesion kinase/Src family kinase axis-mediated tyrosine phosphorylation of metabolic enzymes facilitates tumor metastasisSignal transduction and targeted therapySeptember 1, 2025Chen J, Zhang J, Zhu Y, Zhu Y, Pang J, Wu Q, Wang Y, Zhan Q.nm. Pyruvate assay The level of pyruvate in ESCC cells was assessed via a pyruvate assay kit (Abcam; Cat # ab65342). Briefly, ESCC cells were harvested and resuspended in pyruvate assay buffer, and then centrifuged

(9) β-suppressor protein 1 (ARRB1)-△exon13 modulates the progression of glioblastoma via combination with glycolysis-related proteinsBiochemistry and Biophysics ReportsJune 1, 2025Zi-Long Wei, Shuo Han, Dong-Hua Han, Xue-Tao Li, Yu-Lun Huang, Zhi-Min Wangmeasurement Pyruvate measurement was quantified using a commercially available Pyruvate Assay Kit (Abcam, ab65342). U87 and T98G cells were seeded in six-well plates at a density of 3 × 105 cells per well and incubated

(10) Endoplasmic reticulum stress related super-enhancers suppress cuproptosis via glycolysis reprogramming in lung adenocarcinomaCell Death DisApril 19, 2025Yan Gu, Hongchang Wang, Wentao Xue, Linjia Zhu, Chenghao Fu, Wenhao Zhang, Guang Mu, Yang Xia, Ke Wei, Jun Wangusing a L-lactate assay kit (# E-BC-K044-M, Elabscience, Wuhan, China), and a pyruvate assay kit (#ab65342, Abcam, UK). Measurement of OCR and ECAR The extracellular acidification rate (ECAR) and oxygen consumption

(11) Lenvatinib-activated NDUFA4L2/IL33/PADI4 pathway induces neutrophil extracellular traps that inhibit cuproptosis in hepatocellular carcinomaCellular oncology (Dordrecht)April 1, 2025Yi N, Zhang L, Huang X, Ma J, Gao J.Subsequently, the levels of intracellular copper (Copper Assay Kit, # ab272528), pyruvate (Pyruvate Assay Kit, #ab65342), and Fe2+ (Iron Assay Kit, #ab83366) were measured using kits from Abcam (Cambridge, UK) according

(12) The Novel Dual GIP and GLP‐1 Receptor Agonist Tirzepatide Attenuates Colon Cancer Development by Regulating Glucose MetabolismAdvanced ScienceMarch 24, 2025Yikai Zhang, Yi Xie, Shenglong Xia, Xinnuo Ge, Jiaying Li, Fang Liu, Fan Jia, Shengyao Wang, Qiao Zhou, Menghan Gao, et al.production was measured using an enzymatic assay (Abcam, ab65331) and a pyruvate assay kit (Abcam, ab65342). All measurements were performed according to the manufacturer's instructions. Glucose Flux Measurements The

(13) The Synergistic and Anticancer Potential of Withania Somnifera (Ashwagandha) Ethanol Extract as an Adjuvant with Doxorubicin in MCF7 Breast Cancer Cell LineAsian Pacific Journal of Cancer PreventionMarch 1, 2025Emad M. Elzayat, Ghada E. Elsamahy, Ghada H. Mansour, Ahmed A. El-Sherif, Nourhan Hassan(Abcam, UK, cat no. ab196994), and Pyruvate Assay Kit (Colorimetric/Fluorometric) (Abcam, UK, Cat no. Ab65342) respectively. Measurements were taken at 450 nm using the ROBONIK P2000 ELISA READER. Statistical

(14) The Synergistic and Anticancer Potential of Withania Somnifera (Ashwagandha) Ethanol Extract as an Adjuvant with Doxorubicin in MCF7 Breast Cancer Cell LineAsian Pac J Cancer PrevJanuary 1, 2025Emad M. Elzayat, Ghada E. Elsamahy, Ghada H. Mansour, Ahmed A. El-Sherif, Nourhan Hassan(Abcam, UK, cat no. ab196994), and Pyruvate Assay Kit (Colorimetric/Fluorometric) (Abcam, UK, Cat no. Ab65342) respectively. Measurements were taken at 450 nm using the ROBONIK P2000 ELISA READER. Statistical

(15) The bacterial quorum sensing signal 2’-aminoacetophenone rewires immune cell bioenergetics through the Ppargc1a/Esrra axis to mediate tolerance to infectioneLifeSeptember 13, 2024Arijit Chakraborty, Arunava Bandyopadhaya, Vijay K Singh, Filip Kovacic, Sujin Cha, William M Oldham, A Aria Tzika, Laurence G Rahmewere determined in cellular fractions and mitochondrial fractions by using the Pyruvate Assay Kit (#ab65342, Abcam) according to the manufacturer’s instructions. Briefly, after deproteinization using perchloric

(16) The bacterial quorum sensing signal 2’-aminoacetophenone rewires immune cell bioenergetics through the Ppargc1a/Esrra axis to mediate tolerance to infectionElifeSeptember 13, 2024Arijit Chakraborty, Arunava Bandyopadhaya, Vijay K Singh, Filip Kovacic, Sujin Cha, William M Oldham, A Aria Tzika, Laurence G Rahmewere determined in cellular fractions and mitochondrial fractions by using the Pyruvate Assay Kit (#ab65342, Abcam) according to the manufacturer’s instructions. Briefly, after deproteinization using perchloric

(17) Pyruvate metabolism dictates fibroblast sensitivity to GLS1 inhibition during fibrogenesisJCI insightAugust 13, 2024Contento G, Wilson JA, Selvarajah B, Platé M, Guillotin D, Morales V, Trevisani M, Pitozzi V, Bianchi K, Chambers RC.levels following lactate supplementation were quantified using an enzyme-based fluorometric assay (ab65342 Abcam), and the protocol was followed according to the manufacturer’s instructions. PCLS. Freshly

(18) Histone-methyltransferase KMT2D deficiency impairs the Fanconi anemia/BRCA pathway upon glycolytic inhibition in squamous cell carcinomaNature CommunicationsAugust 8, 2024Wei Liu, Hongchao Cao, Jing Wang, Areeg Elmusrati, Bing Han, Wei Chen, Ping Zhou, Xiyao Li, Stephen Keysar, Antonio Jimeno, et al.HNSCC cells were detected using the glycolytic metabolite detection kits (Abcam, Cat#ab174097 and Cat#ab65342, respectively) according to the manufacturer’s instructions. Cell apoptosis and cell viability assay HNSCC

(19) Mycobacterium tuberculosis suppresses host antimicrobial peptides by dehydrogenating L-alanineNature communicationsMay 17, 2024Peng C, Cheng Y, Ma M, Chen Q, Duan Y, Liu S, Cheng H, Yang H, Huang J, Bu W, et al.filter (Sigma-Aldrich Co. LLC.) before quantification of pyruvate level with Pyruvate Assay Kit (Abcam, ab65342). Clinical samples All the TB patients providing blood samples were from Shanghai Pulmonary Hospital

(20) Mechanosensitive channel of large conductance enhances the mechanical stretching-induced upregulation of glycolysis and oxidative metabolism in Schwann cellsCell Communication and SignalingFebruary 1, 2024Fangzhen Shan, Nannan Zhang, Xiaoying Yao, Yi Li, Zihao Wang, Chuanji Zhang, Yuzhong Wanghigh-sensitivity assay kits (for glucose-6-phosphate, Sigma, MAK021, Taufkirchen, Germany; for pyruvate, Abcam, ab65342, MA, USA; for lactate, Abcam, ab65330, MA, USA) according to the manufacturer’s instructions, respectively.

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Pyruvate Assay Kit from Abcam

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Pyruvate Assay Kit from Abcam

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