Pyruvate Assay Kit ab65342 from Abcam

Product Specs
ItemPyruvate Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65342
Quantity100 Test
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay Time0h 40m
SensitivityMore than 1 µM
Detection Range1 - 200 µM
Research AreaMetabolism

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Phone: +44 (0) 1223 696000 (option 1)
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+44 (0) 1223 771600

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Website: http://www.abcam.com

(41) Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effectsCellular and molecular life sciences : CMLSMarch 1, 2021Christofi M, Le Sommer S, Mölzer C, Klaska IP, Kuffova L, Forrester JV.samples were tested for cellular uptake of glucose (cat no. ab102517), generation of pyruvate (cat no. ab65342), glucose-6-phosphate (G6P) (cat. no. ab83426), succinate (cat no. ab204718), and glycogen (cat no.

(42) Regulation of pyruvate dehydrogenase complex related to lactate switch in CHO cellsEngineering in Life SciencesMarch 1, 2021Johannes Möller, Krathika Bhat, Lotta Guhl, Ralf Pörtner, Uwe Jandt, An‐Ping Zengmetabolites Intracellular pyruvate (cPyr) and acetyl‐CoA (cAcCoA) concentrations were quantified with assay kits (#ab65342 and #ab87546, Abcam, USA). The cells harvested from the bioreactor (≈2·106 cells) were washed with PBS

(43) Long noncoding RNA FIRRE contributes to the proliferation and glycolysis of hepatocellular carcinoma cells by enhancing PFKFB4 expressionJournal of CancerJanuary 1, 2021Shen C, Ding L, Mo H, Liu R, Xu Q, Tu K.lactate production, and glucose consumption in HCC cells were determined using the pyruvate assay kit (ab65342, Abcam, Cambridge, MA, USA), lactate assay kit (ab65331, Abcam), and glucose assay kit (ab136955, Abcam),

(44) Long non-coding RNA MSC-AS1 facilitates the proliferation and glycolysis of gastric cancer cells by regulating PFKFB3 expressionInternational Journal of Medical SciencesJanuary 1, 2021Xianzhen Jin, Lina Qiao, Hui Fan, Chunyan Liao, Jianbao Zheng, Wei Wang, Xiuqin Ma, Min Yang, Xuejun Sun, Wei Zhaokit (ab136955, Abcam, Cambridge, MA, USA), lactate assay kit (ab65331, Abcam), and pyruvate assay kit (ab65342, Abcam) for measuring glucose consumption, lactate production, and pyruvate production according to

(45) Decreased Mitochondrial Function, Biogenesis, and Degradation in Peripheral Blood Mononuclear Cells from Amyotrophic Lateral Sclerosis Patients as a Potential Tool for Biomarker ResearchMolecular NeurobiologyDecember 1, 2020Beatriz Grisolia Araujo, Luiz Felipe Souza e Silva, Jorge Luiz de Barros Torresi, Amanda Siena, Berenice Cataldo Oliveira Valerio, Mariana Dutra Brito, Tatiana Rosado Rosenstockenergetic compounds were measured using commercially available kits following (Abcam, ab83355, ab83359, ab65342, ab65330). Statistical Analysis Results are expressed as mean ± standard deviation (SD) of the number

(46) Pyruvate anaplerosis is a mechanism of resistance to pharmacological glutaminase inhibition in triple-receptor negative breast cancerBMC CancerDecember 1, 2020Dean C. Singleton, Anne-Lise Dechaume, Pamela M. Murray, William P. Katt, Bruce C. Baguley, Euphemia Y. LeungAliquots of the conditioned culture medium were diluted 1:3 in Pyruvate Assay Buffer (Pyruvate Assay Kit ab65342, Abcam plc) and pyruvate concentrations were quantified according to the instruction booklet. Briefly,

(47) Reagentless Amperometric Pyruvate Biosensor Based on a Prussian Blue- and Enzyme Nanoparticle-Modified Screen-Printed Carbon ElectrodeACS OmegaNovember 24, 2020Dinakaran Thirumalai, Seonghye Kim, Suhkmann Kim, Seung-Cheol Changconcentration in the fish serum was also determined using a colorimetric pyruvate assay kit (cat. no. ab65342; Abcam) according to the manufacturer’s protocol.

(48) Rehabilitation Improves Mitochondrial Energetics in Progressive Multiple Sclerosis: The Significant Role of Robot-Assisted Gait Training and of the Personalized IntensityDiagnostics (Basel)October 17, 2020Fabio Manfredini et al.450 nm was measured. Serum levels of pyruvate were determined using the pyruvate assay kit (Abcam, ab65342). Here, 10 µL of serum sample plus 40 µL of pyruvate assay buffer was added to 96-plate wells with a

(49) Nobiletin inhibits cell growth through restraining aerobic glycolysis via PKA‐CREB pathway in oral squamous cell carcinomaFood Science & NutritionJuly 1, 2020Chong‐Xiang Lin, Cheng‐Wei Tu, Yi‐Ke Ma, Peng‐Cheng Ye, Xia Shao, Zhao‐An Yang, Yi‐Ming Fang(ab139418), Glucose Uptake Assay Kit (ab136955), L‐Lactate Assay Kit (ab65331), Pyruvate Assay Kit (ab65342), and pyruvate dehydrogenase (PDH) Enzyme Activity Microplate Assay Kit (ab109902) were purchased from

(50) Staphylococcus aureus PhoU Homologs Regulate Persister Formation and VirulenceFrontiers in MicrobiologyMay 26, 2020Yongpeng Shang et al.Detection Glucose and pyruvate were measured using the Glucose Assay Kit (ab65333; Abcam) and Pyruvate Assay Kit (ab65342; Abcam) according to the manufacturer’s instructions (Liang et al., 2018; Schnack et al., 2019). S.

(51) An engineered enzyme that targets circulating lactate to alleviate intracellular NADH:NAD+ imbalanceNature BiotechnologyMarch 1, 2020Anupam Patgiri et al.produced in the reaction were then measured from the filtrate by using a pyruvate assay kit (Abcam ab65342) following the manufacturer’s instructions. Measurement of LOXCAT’s ability to consume O2 Oxygen consumption

(52) Mitochondrial Dysfunction and Changes in High-Energy Compounds in Different Cellular Models Associated to Hypoxia: Implication to SchizophreniaScientific ReportsDecember 2, 2019Luiz Felipe Souza e Silva, Mariana Dutra Brito, Jéssica Mayumi Camargo Yuzawa, Tatiana Rosado RosenstockNADH141,145–147. All of them were measured using commercially available kits (Abcam, ab83355, ab83359, ab65342, ab65330 and ab65348). For the analysis, astrocytes (from Wistar and SHR pups) were plated at the concentration

(53) Non-enzymatic hydrogen sulfide production from cysteine in blood is catalyzed by iron and vitamin B6Communications BiologyDecember 1, 2019Jie Yang, Paul Minkler, David Grove, Rui Wang, Belinda Willard, Raed Dweik, Christopher HineSimilarly, kinetic pyruvate production was measured using a plate-based pyruvate assay kit (Abcam #ab65342). Briefly, in a clear-bottom 96-well plate, 50 µL of H2S reaction mixture (10 mM L-cys, 1 mM PLP ± 50

(54) Enhanced mitochondrial biogenesis is associated with the ameliorative action of creatine supplementation in rat soleus and cardiac musclesExperimental and Therapeutic MedicineNovember 7, 2019Mennatallah A Gowayed, Shimaa A Mahmoud, Yousria El‑Sayed, Nehal Abu‑Samra, Maher A Kamel(cat. no. ab65331; Abcam) and pyruvate were assayed using colorimetric Pyruvate Assay Kit (cat. no. ab65342; Abcam) according to the manufacturer's protocols. Serum urea (cat. no. UR3825), creatinine (cat. no.

(55) ACTL6A regulates follicle-stimulating hormone-driven glycolysis in ovarian cancer cells via PGK1Cell death & diseaseOctober 24, 2019Zhang J, Zhang J, Wei Y, Li Q, Wang Q.replicates. Measurement of pyruvate level Pyruvate level was determined using Pyruvate Assay Kit (ab65342, Abcam, Cambridge, MA, USA) according to the manufacturer’s instruction. Measurements were performed

(56) Glycolysis upregulation is neuroprotective as a compensatory mechanism in ALSeLifeJune 10, 2019Manzo E, Lorenzini I, Barrameda D, O'Conner AG, Barrows JM, Starr A, Kovalik T, Rabichow BE, Lehmkuhl EM, Shreiner DD, et al.experimental groups. Pyruvate measurements Pyruvate was measured using the Pyruvate Assay Kit (Abcam-ab65342) as per manufacturer’s instructions. In brief, 20 3rd instar larvae per genotype were analyzed in three

(57) The AHR regulates metabolic reprogramming to promote SIRT1-dependent keratinocyte differentiationJournal of Investigative DermatologyApril 1, 2019Carrie Hayes Sutter, Kristin M. Olesen, Jyoti Bhuju, Zibiao Guo, Thomas R. Sutterphosphate buffered saline and intracellular pyruvate was determined by the Fluorescent Pyruvate Assay Kit [ab65342, Abcam (Cambridge, MA)], according to the manufacturer’s instructions. Pyruvate concentrations were

(58) Reactivation of Dihydroorotate Dehydrogenase-Driven Pyrimidine Biosynthesis Restores Tumor Growth of Respiration-Deficient Cancer CellsCell metabolismFebruary 5, 2019Bajzikova M, Kovarova J, Coelho AR, Boukalova S, Oh S, Rohlenova K, Svec D, Hubackova S, Endaya B, Judasova K, et al.XF Cell Mito Stress Test Kit,Agilent,103015-100 Seahorse XF Glycolysis Stress TestKit,Agilent,103020-100 Colorimetric pyruvate assay,Abcam,ab65342 Pierce BCA Protein Assay Kit,Thermo Fisher Scientific,#23225 Atto488 NT Labeling Kit,Jena Bioscience,PP-305S-488 Atto550

(59) 果蝇幼虫脂肪组织丙酮酸 (Pyruvate) 检测 (试剂盒)BIO-PROTOCOLJanuary 1, 2019梦瑶许, 勋黄(Amicon Ultra centrifugal filters 10K) 4. 果蝇 3 龄幼虫 5. 液氮 6. 丙酮酸检测试剂盒 (Abcam, catalog number: ab65342) 7. Brandford Reagent (Sigma, catalog number: B6916-500ML) 8. BSA (阿拉丁, catalog number: A104912-100g) 仪器设备 1. 移液枪 2. 研磨器

(60) Intra-cellular lactate concentration in T lymphocytes from septic shock patients — a pilot studyIntensive Care Medicine ExperimentalDecember 1, 2018Thibaut Girardot, Thomas Rimmelé, Guillaume Monneret, Julien Textoris, Fabienne Venetwas measured using a commercially available kit from the same manufacturer (Pyruvate Assay Kit, ref. ab65342, Abcam) followed by a fluorometric measurement (Ex/Em 540/590 nm). mHLA-DR, CD4+, and T regulatory

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Pyruvate Assay Kit from Abcam

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Pyruvate Assay Kit from Abcam

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