Pyruvate Assay Kit ab65342 from Abcam

Product Specs
ItemPyruvate Assay Kit
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab65342
Quantity100 Test
MethodFuncS
Sample TypeCell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts
Assay Time0h 40m
SensitivityMore than 1 µM
Detection Range1 - 200 µM
Research AreaMetabolism

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+44 (0) 1223 771600

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Website: http://www.abcam.com

(21) The effects of long-term lactate and high-intensity interval training (HIIT) on brain neuroplasticity of aged miceHeliyonJanuary 30, 2024Zhou Lei, Soroosh Mozaffaritabar, Takuji Kawamura, Atsuko Koike, Attila Kolonics, Johanna Kéringer, Ricardo A. Pinho, Jingquan Sun, Ruonan Shangguan, Zsolt Radákexercise and lactate intervention was assayed using pyruvate and lactate assay kits (Abcam; ab65330, ab65342). About 40 ± 2 mg of the homogenized left hemisphere of seven mice of each group were weighed and lysed

(22) PFKP deubiquitination and stabilization by USP5 activate aerobic glycolysis to promote triple-negative breast cancer progressionBreast Cancer ResearchJanuary 12, 2024Zi-Mei Peng, Xiao-Jian Han, Tao Wang, Jian-Jun Li, Chun-Xi Yang, Fang-Fang Tou, Zhen Zhangmanufacturer's instructions, with all results being normalized to cell number. The Pyruvate Assay Kit (Abcam, ab65342) and Lactate Assay Kit (Abcam, ab65331) were utilized to perform the pyruvate and lactate assay, respectively.

(23) The novel roles of YULINK in the migration, proliferation and glycolysis of pulmonary arterial smooth muscle cells: implications for pulmonary arterial hypertensionBiol ResDecember 7, 2023Yi-Chia Wu, Wei-Ting Wang, Ming-Chun Yang, Yu-Tsun Su, Jwu-Lai Yeh, Jong-Hau Hsu, Jiunn-Ren Wupyruvic acid. In this research, we determined pyruvate production by using the Pyruvate Assay Kit (ab65342, Abcam) following the manufacturer’s guidelines to examine the correlation between the level of YULINK

(24) Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in miceNature CommunicationsOctober 10, 2023Tomas Venit, Oscar Sapkota, Wael Said Abdrabou, Palanikumar Loganathan, Renu Pasricha, Syed Raza Mahmood, Nadine Hosny El Said, Shimaa Sherif, Sneha Thomas, Salah Abdelrazig, et al.experiment. Metabolites measurement For metabolites measurements, L-Lactate Assay Kit (ab65330), Pyruvate Assay Kit (ab65342), ADP assay kit (ab83359), ATP Assay Kit (ab83355), and Hydrogen Peroxide Assay Kit (ab102500) were

(25) Pantothenate Kinase 4 Governs Lens Epithelial Fibrosis by Negatively Regulating Pyruvate Kinase M2-Related GlycolysisAging and diseaseOctober 1, 2023Li X, Luo LL, Li RF, Chen CL, Sun M, Lin S.method. Pyruvate detection The PA levels were determined by the pyruvate assay kit (Pyruvate Assay Kit (ab65342/K609-100), Abcam). The PA content was evaluated by measuring the absorbance at 505 nm, and the PA content

(26) Effect of Moderate-Intense Training and Detraining on Glucose Metabolism, Lipid Profile, and Liver Enzymes in Male Wistar Rats: A Preclinical Randomized StudyNutrientsAugust 31, 2023Hira Shakoor, Jaleel Kizhakkayil, Mariyam Khalid, Amar Mahgoub, Carine Platat15 min at 13,000× g at 4 °C. Pyruvate was determined in the supernatant using a pyruvate assay kit (ab65342, Abcam, Cambridge, UK). 2.11. Lactate In AT4, blood lactate concentration was measured at T4 and T12.

(27) Effects of Tofacitinib on Muscle Remodeling in Experimental Rheumatoid SarcopeniaInternational journal of molecular sciencesAugust 24, 2023Bermejo-Álvarez I, Pérez-Baos S, Gratal P, Medina JP, Largo R, Herrero-Beaumont G, Mediero A.Iberica Instrumentacion S.L, Barcelona, Spain). Pyruvate was measured using a pyruvate assay kit (Abcam, ab65342) following recommendations and measured in a microplate reader at 570 nm (Tecan Infinite). 4.9. Statistical

(28) Lambda-cyhalothrin-induced pancreatic toxicity in adult albino ratsScientific ReportsJuly 18, 2023Samar Sakr, Walaa A. Rashaddetermined colorimetrically using L-lactate Assay kits (cat. no. ab65331) and Pyruvate Assay Kits (cat. no. ab65342) of Abcam, according to the manufacturer’s instructions. Histopathology Hematoxylin and Eosin (H&E)

(29) ME2 Promotes Hepatocellular Carcinoma Cell Migration through PyruvateMetabolitesApril 10, 2023Yang Y, Zhang Z, Li W, Li L, Zhou Y, Du W.with or without 1 mM dimethyl-pyruvate for 48 h. Pyruvate levels were analyzed by pyruvate assay kit (ab65342, Abcam, Cambridge, UK), following the manufacturer’s instructions. 2.7. Wound Healing Assay Cells

(30) Melatonin inhibits bladder tumorigenesis by suppressing PPARγ/ENO1-mediated glycolysisCell Death & DiseaseApril 6, 2023Dexin Shen, Zhao Deng, Wei Liu, Fenfang Zhou, Yayun Fang, Danni Shan, Gang Wang, Kaiyu Qian, Mengxue Yu, Yi Zhang, et al.subsequent steps were performed according to the user’s instructions for the Pyruvate Assay Kit (Abcam, ab65342). Dual-luciferase reporter assay 293 T cells were added to 24-well plates 24 h before transfection.

(31) Design, Synthesis and Biological Evaluation of Quinoline-8-Sulfonamides as Inhibitors of the Tumor Cell-Specific M2 Isoform of Pyruvate Kinase: Preliminary StudyMoleculesMarch 9, 2023Krzysztof Marciniec, Zuzanna Rzepka, Elwira Chrobak, Stanisław Boryczka, Małgorzata Latocha, Dorota Wrześniok, Artur Beberokactivity was determined using commercially available enzyme assay kit: Pyruvate Assay Kit (Cat. No. ab65342; Abcam, USA), according to the manufacturer’s instruction. In brief, pyruvate concentration in cell

(32) Damage-induced pyroptosis drives endogenous thymic regeneration via induction of Foxn1 by purinergic receptor activationbioRxivJanuary 25, 2023Sinéad Kinsella, Cindy A. Evandy, Kirsten Cooper, Antonella Cardinale, Lorenzo Iovino, Paul deRoos, Kayla S. Hopwo, Colton W. Smith, David Granadier, Lucas B. Sullivan, et al.intracellular pyruvate and lactate levels were measured by absorbance using Pyruvate Assay kit (Abcam, ab65342) or Lactate-Glo™ Assay (Promega, J5021). Thymocytes were isolated from untreated and TBI-treated mice

(33) Inhibition of glutaminolysis restores mitochondrial function in senescent stem cellsCell reportsNovember 29, 2022Choudhury D, Rong N, Ikhapoh I, Rajabian N, Tseropoulos G, Wu Y, Mehrotra P, Thiyagarajan R, Shahini A, Seldeen KL, et al.Kit,Abcam,Cat# ab83431 PicoProbe Glutaminase Assay Kit,Biovision,Cat# K455-100 Pyruvate Assay Kit,Abcam,Cat# ab65342 Ammonia Assay Kit,Abcam,Cat# ab83360 Urea Assay Kit,Abcam,Cat# ab83362 DCF ROS/RNS Assay Kit,Abcam,Cat#

(34) SET domain containing 2 (SETD2) influences metabolism and alternative splicing during myogenesisThe FEBS journalNovember 1, 2022Wiedner HJ, Torres EV, Blue RE, Tsai YH, Parker J, Giudice J.protein. Intracellular pyruvate quantification. Pyruvate was measured with the Pyruvate Assay Kit from Abcam (ab65342) according to manufacturer’s instructions. Briefly, transfected cells were harvested in assay buffer

(35) Long-term effects of early postnatal stress on Sertoli cellsFrontiers in GeneticsOctober 24, 2022Kristina M. Thumfart, Samuel Lazzeri, Francesca Manuella, Isabelle M. Mansuywere measured in the protein-depleted flow-through using assay kits (MAK064-1KT, Sigma-Aldrich) and (ab65342, Abcam) according to the manufacturer’s instructions. Each sample was run twice and fluorescence was

(36) Malic Enzyme 1 Absence in Synovial Sarcoma Shifts Antioxidant System Dependence and Increases Sensitivity to Ferroptosis Induction with ACXT-3102Clinical cancer research : an official journal of the American Association for Cancer ResearchAugust 15, 2022Brashears CB, Prudner BC, Rathore R, Caldwell KE, Dehner CA, Buchanan JL, Lange SES, Poulin N, Sehn JK, Roszik J, et al.cytoplasmic fraction was then accessed according to the manufacturer's protocol (Pyruvate Assay Kit, ab65342; Abcam). This protocol was adapted by substituting the pyruvate enzyme mix with malate enzyme mix composed

(37) Circular RNA PLCE1 promotes epithelial mesenchymal transformation, glycolysis in colorectal cancer and M2 polarization of tumor-associated macrophagesBioengineeredMarch 1, 2022Yi B, Dai K, Yan Z, Yin Z.and glucose consumption in SW480 cells was via the pyruvate, lactic acid, and glucose detection kits (ab65342, ab65331, ab136955, Abcam) obeying the manufacturer’s agreement. 2.9 Western blot Conduction of Radio-Immunoprecipitation assay

(38) The Critical Role Played by Mitochondrial MITF Serine 73 Phosphorylation in Immunologically Activated Mast CellsCellsFebruary 8, 2022Paruchuru LB, Govindaraj S, Razin E.estimated using the ELISA kits from Thermo Fisher Scientific, San Diego, CA, USA. Pyruvate levels (ab65342) and PDH activity (ab109902) were estimated using the kits from Abcam (Cambridge, UK) according to the

(39) Salmonella Typhimurium reprograms macrophage metabolism via T3SS effector SopE2 to promote intracellular replication and virulenceNature CommunicationsDecember 1, 2021Lingyan Jiang et al.the manufacture’s manual. Pyruvate concentration was measured by using a Pyruvate assay kit (Abcam #ab65342) according to the manufacture’s manual. Results were normalized to the cell number. Western blotting Protein

(40) MPV17 Mutations Are Associated With a Quiescent Energetic Metabolic ProfileFrontiers in Cellular NeuroscienceMarch 17, 2021Sandra Jacinto, Patrícia Guerreiro, Rita Machado de Oliveira, Teresa Cunha-Oliveira, Maria João Santos, Manuela Grazina, Ana Cristina Rego, Tiago F. Outeiromeasured in the culture medium with L-Lactate Assay Kit (Abcam, #ab65330) and Pyruvate Assay Kit (Abcam, ab65342), respectively, in 96-well plates, according to the manufacturer’s instructions. Statistics Statistical

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Pyruvate Assay Kit from Abcam

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Pyruvate Assay Kit from Abcam

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