PerCP/Cyanine5.5 anti-mouse CD4 100540 from BioLegend

Product Specs
ItemPerCP/Cyanine5.5 anti-mouse CD4
CompanyBioLegend
Price $283.00Supplier Page View Company Product Page
Catalog Number100540
NCBI Gene AliasesL3T4, Ly-4
HostRat
ApplicationsFC - Quality tested
IsotypeRat IgG2a, kappa
Quantity100 µg
ReactivityMouse
CloneRM4-5
Conjugate/TagPerCP/Cyanine5.5
Antibody TypePrimary
NCBI Full Gene NameCD4 antigen
Concentration0.2mg/ml
FormatPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
ImmunogenBALB/c mouse thymocytes
ClonalityMonoclonal
TargetCD4

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Artificial exosomes synergistically reshape sepsis immune homeostasis by modulating neutrophil fate and blocking PD-1/PD-L1Cell Reports MedicineJune 16, 2026Pengcheng Zhang, Yahui Gao, Yaxin Wang, Xin Li, Yi Jiang, Yigang Xu, Keliang Xie, Yixuan Ma, Sheng Wang, Hui Zheng, et al.Antibody,Biolegend (USA),Cat. No. 100204 PerCP/Cyanine5.5 anti-mouse CD4 Antibody,Biolegend (USA),Cat. No. 100540 APC anti-mouse CD8a Antibody,Biolegend (USA),Cat. No. 162306 PE/Cyanine7 anti-mouse CD45 Antibody,Biolegend

(2) Enhancing Immunotherapeutic Response in Colorectal Cancer with a Neuropilin 1–Targeting Tumor-Penetrating PeptideCancer Res CommunJune 1, 2026Atsushi Fukugaki, Shigeo Hisamori, Dean Thumkeo, Hotaka Kawai, Yugo Matsui, Yuki Teramoto, Yoshihisa Okuchi, Atsuhito Omori, Norio Miyamura, Yukihito Kuroda, et al.with antibodies against CD45 (Thermo Fisher Scientific, 11-0451-82, RRID: AB_465050), CD4 (BioLegend, 100540, RRID: AB_893326), CD8a (BioLegend, 100712, RRID: AB_312751), and CD25 (Thermo Fisher Scientific, 12-0251-82,

(3) Defining critical roles for ZBP1 in PANoptosis utilizing a novel genetic tool for disease modeling and therapeutic developmentbioRxivMay 25, 2026Alexander P. Young, Twinu Wilson Chirayath, Yaqiu Wang, Sangappa B. Chadchan, Thirumala-Devi Kannegantiantibodies: anti-CD45.2-PE/Cy7 (Clone 104, Biolegend, 109830), anti-CD4-PerCP/Cy5.5 (Clone RM4–5, Biolegend, 100540), anti-CD8-FITC (Clone53-6.7, Biolegend, 100706), anti-CD19-APC/Cy7 (Clone 1D3, Biolegend, 152411),

(4) Bacterial specificity of the gut microbiome predicts bone density in primary hyperparathyroidismBone ResMay 25, 2026Hamid Y. Dar, Jiali Fang, Sanchiti Patil, Nand K. Roy, Sanchita Agarwal, M. Neale Weitzmann, Rheinallt M. Jones, John P. Bilezikian, Roberto PacificiH57-597, catalog 109230), AF 700-CD3 (clone 17A2, catalog 100216), PerCP/Cy5.5-CD4 (clone RM4-5, catalog 100540), BV 711-CD8 (clone 53-6.7, catalog 100748) (Biolegend) and FITC-Vβ T cell receptor (clone RR4-7, catalog

(5) Targeted enzymatic therapy for coeliac diseaseEMBO Molecular MedicineMay 14, 2026Marina Girbal-González, Arturo Rodríguez-Banqueri, Hadeel Swaid, Soraia R Mendes, Laura Garzón-Flores, Juan Sebastián Ramírez-Larrota, Carolina Cueva, M Victoria Moreno-Arribas, Christof Regl, Christian G Huber, et al.ELISA,AMSBIO,AMS.E03A2131 Mouse anti-CD3-APC/Cyanine7,BioLegend,100221 Mouse anti-CD4-PerCP/Cyanine5.5,BioLegend,100539 Mouse anti-CD8α-FITC,BioLegend,100803 Mouse anti-CD44-PE,BioLegend,103023 Mouse anti-CD62L-APC,BioLegend,104411 Mouse

(6) Immunogenic tumor cell death and T-cell-derived IFN-γ elicit tumoricidal macrophages to potentiate OX40 immunotherapyCell Reports MedicineApril 21, 2026Yanqin Liu, Jiliang Zhao, Kailu Yang, Qiongqiong Ma, Dongping Zhang, Lili Xu, Zhaoyuan Zhang, Zhongqian Yin, Jingru Chen, Yi Wang, et al.17A2),Biolegend,"Cat#100204, RRID: AB_312661" PerCP/Cyanine5.5 anti-mouse CD4 (clone: RM4-5),Biolegend,"Cat#100540, RRID: AB_893326" Brilliant Violet 510™ anti-mouse CD8α (clone: 53-6.7),Biolegend,"Cat#100751, RRID:

(7) Female-enriched Eggerthella lenta drives neuroinflammation and IFN-γ via host receptor TLR2bioRxivMarch 25, 2026Rachel R. Rock, Margaret Alexander, Cecilia Noecker, Kai R. Trepka, Vaibhav Upadhyay, Edwin F. Ortega, Lorenzo Ramirez, Lena Siewert, Christine A. Olson, Taylor Halsey, et al.Biosciences,Cat#: BDB740844; RRID:AB_2740467 "anti-CD4 (Clone RM4-5, PerCP-Cy5.5)",BioLegend,Cat#: 100540; RRID:AB_312741 "anti-CD3 (Clone 17A2, FITC)",Thermo Fisher Scientific,Cat#: 50-112-9303; RRID:AB_2533944 "anti-FoxP3

(8) Hormonal rewiring of immunity during dietary restriction ensures host defense and systemic glucose conservationImmunityMarch 10, 2026Luisa Menezes-Silva, Mingeum Jeong, Charles Carr, Riley M. Schneider, Silvia Pires, Ana C. Codo, Jazib Uddin, Alexander Grier, Randy S. Longman, Niels Olsen Saraiva Saraiva Camara, et al.(RM4-5)",Biolegend,Cat# 100531; RRID: AB_493374 "Anti-mouse CD4, PerCP/Cyanine5.5 (RM4-5)",Biolegend,Cat# 100540; RRID: AB_493374 "Anti-mouse CD5, Biotin (53-7.3)",Biolegend,Cat# 100604; RID: AB_312733 "Anti-mouse

(9) Decorin facilitates T cell-mediated antitumor immunity and augments the efficacy of anti-PD1 immunotherapyCancer Immunology, ImmunotherapyJanuary 27, 2026Ningqian Zheng, Lvzhu Xiang, Guiqin Xu, Yun Liu, Chen Xu, Li Zhang, You Zuo, Zhiqian Ye, Yongzhong Liu, Zhaojuan YangeBioscience), CD8-FITC (53–0081-82, eBioscience), CD8-PE-Cy7 (25–0081-82, eBioscience), CD4-PerCP-Cy5.5 (100540, BioLegend), CD107a-APC (121613, BioLegend), perforin-FITC (154309, BioLegend), IFN-γ-APC (17–7311-82,

(10) Antibody-induced internalization and degradation of PLA2R amplifies CD4+ T cell activationTheranosticsJanuary 1, 2026Yuning Liu, Yinyin Li, Hui Zhou, Jieli Yu, Pei Deng, Weiwei Xu, Bingqian Pan, Lei Zhang, Hong Zhou, Jing Zhang, et al.Thermo Fisher Scientific); alpha-Tubulin (66031-1, Proteintech); GAPDH (60004-1, Proteintech); PerCP/Cy5.5 anti-mouse CD4 (100540, Biolegend); APC anti-mouse CD25 (102012, Biolegend); PE/Cy7 anti-mouse CD69 (104512, Biolegend); PerCP/Cy5.5

(11) Modifying non-coding regions of linear DNA vaccines to explore the interplay of expression and inflammation in immunogenicityHuman Vaccines & ImmunotherapeuticsDecember 31, 2025David C. Stirling, Maria de Miguel Ferrer, Sungwon Kim, Madina Wane, Daniel Kysh, Lisa J. Caproni, John S. Tregoninganti-CD8 (Clone: 53–6.7, Cat: 560247 BD Biosciences), PerCP-Cy5.5 anti-mouse CD4 (Clone: RM4-5, Cat: 100540 BioLegend), BV711 anti-mouse CD44 (Clone: IM7, Cat: 103057 BioLegend), PE-Cy7 anti-mouse CD62L (Clone:

(12) Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responsesCellDecember 24, 2025Diana Castaño, Emily Bettini, Binod Kumar, Aleksey Chudnovskiy, Anna Siv, Giulia Protti, Sandra Nakadakari-Higa, Simona Ceglia, Nina De Luna, Joy E. Chiu, et al.,BD Biosciences,Cat#563565 "CD3e, clone 145-2c11, BV785",BioLegend,Cat#100355 "CD4, clone RM4-5, PerCP-Cy5.5",BioLegend,Cat#100540 "CD4, clone SK3, BUV 563",BD Biosciences,Cat#612912 "CD8a, clone53-6.7, PerCP-Cy5.5",BioLegend,Cat#100734 "CD8a,

(13) Virus envelope glycoprotein targeting bispecific T cell engager protects mice from lethal severe fever with thrombocytopenia virus infectionCell Reports MedicineNovember 18, 2025Xuefang Peng, Wei Wang, Yuexia Liang, Yi Jian, Qiaoyue Wei, Tong Yang, Chaojie Wang, Xiaohong Yin, Mingjuan Du, Hang Shang, et al.anti-mouse CD8,BioLegend,Cat# 100743; RRID: AB_2561352 PerCP/Cyanine5.5 anti-mouse CD4,BioLegend,Cat# 100540; RRID: AB_893326 Brilliant Violet 421 anti-mouse Granzyme B,BioLegend,Cat# 396414; RRID: AB_2810603 Pacific Blue™ anti-mouse IFN-γ

(14) Extracellular vesicle bioactivity and potential for clinical development are determined by mesenchymal stromal cell clonal subtypeStem Cell Res TherOctober 16, 2025Savvas Ioannou, Alasdair G. Kay, Andrew P. Stone, Emma Rand, Samuel Elberfeld, William Bolton, Tony Larson, Rachel E. Crossland, Oksana Kehoe, David A. Mentlak, et al.(AF488), CD3 (APC-Cy7), CD4 (PerCP-Cy5.5), CD62L (APC) and CD44 (PE) (BioLegend, Cat: 109215, 100222, 100540, 104412, 103008). For all tests, Zombie Aqua (BioLegend, Cat: 423101) was used to exclude dead cells. Antigen-induced

(15) Cre-driven tdTomato expression unexpectedly confers resistance to peripheral but not central lupus in dLckCre miceJ Transl AutoimmunSeptember 30, 2025Ning Han, Keer Wang, Dan Yang, Mingxuan Han, Xiaoxiao Hou, Zhenghao Xu65-0865-14, USA), anti-CD3-FITC (BioLegend, catalog # 100203, USA), anti-CD4-PerCP (BioLegend, Cat No. 100539, USA), anti-CD8-KO525 (BioLegend, Cat No. 100752, USA). Data were collected via FACS analysis using

(16) Recombinant Oncolytic Vesicular Stomatitis Virus Expressing Mouse Interleukin-12 and Granulocyte-Macrophage Colony-Stimulating Factor (rVSV-dM51-mIL12-mGMCSF) for Immunotherapy of Lung CarcinomaInternational Journal of Molecular SciencesSeptember 3, 2025Anastasia Ryapolova, Margarita Zinovieva, Kristina Vorona, Bogdan Krapivin, Vasiliy Moroz, Nizami Gasanov, Ilnaz Imatdinov, Almaz Imatdinov, Roman Ivanov, Alexander Karabelsky, et al.stained with the anti-mouse antibodies Alexa Fluor 700 anti-CD3 (100216), PerCP/Cyanine 5.5 anti-CD4 (100540), APC anti-CD8a (155006), Brilliant Violet 421 anti-FOXP3 (126419) (all from BioLegend, San Diego, CA,

(17) Ultrasound spatiotemporally enables prolonged therapeutic mRNA translation in engineered bacteria for enhanced cancer immunotherapyTheranosticsAugust 30, 2025Zhaoyou Liu, Lantian Wang, Jieyuan An, Tian Zhou, Mengying Wei, Guodong Yang, Pengying Wu, Lijun Yuan(100722, BioLegend, USA), APC anti-mouse CD8 (100711, BioLegend, USA), PerCP/Cyanine5.5 anti-mouse CD4 (100540, BioLegend, USA), PE anti-mouse TIM3 (119703, BioLegend, USA), Brilliant Violet 421™ anti-mouse LAG-3

(18) Activated T cells break tumor immunosuppression by macrophage re-educationCancer DiscoveryJuly 3, 2025Rosa Trotta, Silvia Rivis, Shikang Zhao, Marie-Pauline Orban, Sarah Trusso Cafarello, Iris Charatsidou, Joanna Pozniak, Jonas Dehairs, Lotte Vanheer, Carlos A. Pulido Vicuna, et al.AB_398527; BD Biosciences, 612898, RRID: AB_2870186; BioLegend, 100713, RRID: 312752), anti-CD4 (BioLegend, 100540, RRID: AB_893332), anti-CD370 (BioLegend, 143506, RRID: AB_2566380), anti-CD90.1 (BD Biosciences, 202506,

(19) An mRNA lipid nanoparticle-incorporated nanofiber-hydrogel composite for cancer immunotherapyNat CommunJuly 1, 2025Yining Zhu, Zhi-Cheng Yao, Shuyi Li, Jingyao Ma, Christine Wei, Di Yu, Jessica L. Stelzel, Bobby Y. X. Ni, Yang Miao, Kyra Van Batavia, et al.(BioLegend # 100706, 100712, BD Biosciences # 747502); PerCP-Cyanine 5.5 anti-mouse CD4 (BioLegend # 100540); PE anti-mouse IFN-γ (BioLegend # 505808); Brilliant Violet 421 anti-mouse IL-4 (BioLegend # 504120);

(20) The immune response against cancer is modulated by stromal cell fibronectinNeoplasiaJune 30, 2025Alexander Lubosch, Lauren Pitt, Caren Zoeller, Franziska Wirth, Tarik Exner, Barbara Steigenberger, Guido Wabnitz, Jutta Schroeder-Braunstein, Inaam A. NakchbandiBDBiosciences,1:1001:100,AB_312915AB_2737997 CD3ε,PE,#100308 Biolegend,1:400,AB_312673 CD4,PerCP-Cy5.5,#100540 Biolegend,1:400,AB_893326 CD44,APC-Cy7,#103028 Biolegend,1:100,AB_312993 CD45,Alexa Fluor 647APC-Cy7Pacific

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1/29/2021BioLegend PerCP-Cy5.5 Anti-mouse CD4 Ab Kung-Chi KaoWorks well.
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9/29/2017Mouse CD4 Antibody For Flow Cytometry Jeeho LeeThe aim was to study T helper cell subsets. The specific goal was to examine distinct CD4+ T cell populations such as Th1, Th2, Th17 and Treg. I selected this product because it was of high quality ... Read Review

PerCP/Cyanine5.5 anti-mouse CD4 from BioLegend

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PerCP/Cyanine5.5 anti-mouse CD4 from BioLegend

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