BioLegend PerCP-Cy5.5 Anti-mouse CD4 Ab

January 29, 2021

Department of Fundamental Oncology
University of Lausanne
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

PerCP/Cyanine5.5 anti-mouse CD4 Antibody

Catalog Number:

100540

Clone Number:

RM4-5

To titrate the antibody, 1 x 10^6 splenocytes from C57BL/6 mouse were stained with:PerCP-Cy5.5 anti-CD4 AbAlexa Fluor 700 anti-CD8 AbZombie NIRin 50 μL FACS buffer for 20 min at RT

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Splenocytes from C57BL/6 mouse

Primary Incubation

See description

Blocking Agent

N/A

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

BD LSRFortessa

Results Summary

To titrate the antibody, 1 x 10^6 splenocytes from C57BL/6 mouse were stained with:PerCP-Cy5.5 anti-CD4 AbAlexa Fluor 700 anti-CD8 AbZombie NIRin 50 μL PBS buffer at 4C for 30 min

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Great resolution

The Bad

N/A

The Bottom Line

According to the titration, 1:200 dilution has the best SI.

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