Mouse CD4 Antibody For Flow Cytometry

September 29, 2017

Cell Biology
La Jolla Institute for Allergy and Immunology
Instructor

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

PerCP/Cy5.5 anti-mouse CD4 Antibody

Catalog Number:

100540

The aim was to study T helper cell subsets. The specific goal was to examine distinct CD4+ T cell populations such as Th1, Th2, Th17 and Treg. I selected this product because it was of high quality and is cost effective.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:100 (0.4ul of antibody in 40ul of sample)

Blocking Agent

5% rat serum

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

PerCP-Cy5.5

Results Summary

We were able to detect CD4+ T cell clearly

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

High quality with low cost

The Bad

None

The Bottom Line

I strongly recommend this antibody for flow cytometry

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