Great Mouse Podocalyxin Antibody for Immunofluorescence

March 27, 2023

Anatomy and Cell Biology
University of Illinois at Chicago
Graduate Student

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Company:

R&D Systems

Product Name:

Mouse Podocalyxin Antibody

Catalog Number:

AF1556

Clone Number:

N/A

Blood vessels in mouse cerebellum were identified by immunofluorescence by staining for podocalyxin. Leakage of fibrinogen into the brain parenchyma identified areas of blood-brain barrier damage.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse brain tissue

Primary Incubation

1:500 in PBS + 0.01% Triton-X100, overnight at 4C

Blocking Agent

PBS + 0.01% Triton-X100 + 10% BSA

Secondary Incubation

Goat anti-mouse IgG AF488 1:500 in PBS+0.1% Triton-X100, 2hr at 25C

Tertiary Incubation

N/A

Detection

Epifluorescence microscopy

Results Summary

Blood vessels in the mouse cerebellum were brightly labeled with podocalyxin antibody for immunofluorescence microscopy.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bright staining, inexpensive.

The Bad

None

The Bottom Line

This antibody is great for immunofluorescence staining of blood vessels.

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