VASA Antibody AF2030 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemVASA Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $499.00Supplier Page View Company Product Page
Catalog NumberAF2030
Quantity100 ug (also 25 ug)
ReactivityHamster,Human,Monkey,Mouse,Primate - Macaca mulatta (Rhesus Macaque),Transgenic Mouse,Xenograft
HostGoat
TargetVASA
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
Conjugate/TagUnconjugated
NCBI Full Gene NameDEAD-box helicase 4
ApplicationsWB, FCM, ICC, IF, IHC, IHC-fr, IHC-p
ClonalityPolyclonal
ImmunogenE. coli-derived recombinant human VASA, Met1-Tyr145, Accession # Q9NQI0
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
IsotypeIgG
NCBI Gene AliasesVASA

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human VASA in direct ELISAs and Western blots.

Sizes Available: 100 ug (also 25 ug)

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R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Mouse germline cysts contain a fusome-like structure that mediates oocyte developmenteLifeMarch 11, 2026Madhulika Pathak, Allan C SpradlingRRID:AB_1659152,IF (1:400) Antibody,DDX4,Abcam/R&D systems,ab13840 RRID:AB_443012/ab27591 RRID:AB_11139638/AF2030 RRID:AB_2277369,IF (1:400) Antibody,SSEA1,DSHB,MC-480 RRID:AB_528475,IF (1:1) Culture supernatant Antibody,Gm-130,BD

(2) Mouse germline cysts contain a fusome-like structure that mediates oocyte developmenteLifeMarch 11, 2026Pathak M, Spradling AC.RRID:AB_1659152,IF (1:400) Antibody,DDX4,Abcam/R&D systems,ab13840 RRID:AB_443012/ab27591 RRID:AB_11139638/AF2030 RRID:AB_2277369,IF (1:400) Antibody,SSEA1,DSHB,MC-480 RRID:AB_528475,IF (1:1) Culture supernatant Antibody,Gm-130,BD

(3) Tex11 Mutant Mouse Models of Human AzoospermiabioRxiv : the preprint server for biologyFebruary 18, 2026Atkins GR, Hvasta-Gloria RL, Ausavarungnirun C, Pombar CR, Hardy JJ, Sukhwani M, Barnard EP, Pollock N, Malizio M, Sheng Y, et al.

(4) MEIOC prevents continued mitotic cycling and promotes meiotic entry during mouse oogenesisDevelopmentJanuary 1, 2026Esther G. Ushuhuda, Jenniluyn T. Nguyen, Natalie G. Pfaltzgraff, Shelbie M. Wenner, Matthew Kofron, Maria M. Mikedisab181591; 1.344 mg/ml stock; 1:750 working concentration), anti-DDX4 (goat polyclonal, R&D Systems AF2030; 0.2 mg/ml stock; 1:250 working concentration) and anti-SYCP3 (mouse monoclonal, Santa Cruz Biotechnology

(5) MEIOC prevents continued mitotic cycling and promotes meiotic entry during mouse oogenesisbioRxiv : the preprint server for biologyOctober 15, 2025Ushuhuda EG, Nguyen JT, Pfaltzgraff NG, Wenner SM, Kofron M, Mikedis MM.Abcam ab181591; 1.344 mg/mL stock; 1:750 working concentration) and DDX4 (goat polyclonal, R&D systems AF2030; 0.2 mg/mL stock; 1:250 working concentration). Slides were washed in PBS and incubated in secondary

(6) HELQ Maintains Genome Stability of Primordial Germ Cells by Inhibiting LINE-1 ExpressionFASEB JJune 30, 2025Lili Cao, Jiayi Ren, Zhaojie Kong, Mengchun Hu, Yaxuan Zhang, Zi-Jiang Chen, Yingying Qin, Yajuan Yang, Shidou ZhaoTechnology). The primary antibodies used for immunostaining were anti‐DDX4 (1:50 dilution; Novus Biologicals, AF2030), anti‐FOXL2 (1:250 dilution; Abcam, ab246511), anti‐SOX9 (1:200 dilution; Millipore, AB5535), anti‐cleaved

(7) HELQ Maintains Genome Stability of Primordial Germ Cells by Inhibiting LINE‐1 ExpressionFASEB JJune 21, 2025Lili Cao, Jiayi Ren, Zhaojie Kong, Mengchun Hu, Yaxuan Zhang, Zi‐Jiang Chen, Yingying Qin, Yajuan Yang, Shidou ZhaoTechnology). The primary antibodies used for immunostaining were anti‐DDX4 (1:50 dilution; Novus Biologicals, AF2030), anti‐FOXL2 (1:250 dilution; Abcam, ab246511), anti‐SOX9 (1:200 dilution; Millipore, AB5535), anti‐cleaved

(8) In vitro growth of secondary follicles from cryopreserved-thawed ovarian cortexHuman ReproductionDecember 1, 2024Hui Cheng, Fu Wei, Julieta S Del Valle, Tessa H R Stolk, Judith A Huirne, Joyce D Asseler, Gonneke S K Pilgram, Lucette A J Van Der Westerlaken, Norah M Van Mello, Susana M Chuva De Sousa Lopesprimary antibodies used were goat anti-FOXL2 (1:250, NB100-1277, Bio-Techne, USA), goat anti-DDX4 (1:250, AF2030, R&D, USA), mouse anti-AMH (1:30, MCA2246T, BioRad, USA), rabbit anti-KRT19 (1:150, ab76539, Abcam,

(9) Ovotesticular cords and ovotesticular follicles: New histologic markers for human ovotesticular syndromeJournal of pediatric urologyOctober 1, 2024Baskin LS, Cao M, Li Y, Baker L, Cooper CS, Cunha GR.

(10) VEGF-dependent testicular vascularisation involves MEK1/2 signalling and the essential angiogenesis factors, SOX7 and SOX17BMC BiologyOctober 1, 2024Rheannon O. Blücher, Rachel S. Lim, Matthew E. Ritchie, Patrick S. WesternProtein,Source and catalogue number,Species,Dilution Mouse Vasa Homologue (MVH),"R&D Systems, AF2030",Goat,F: 1/100SIF: 1/400 CD31,"R&D Systems, AF3628",Goat,SIF: 1/100WIF: 1/100 SOX7,"R&D Systems, AF2766",Goat,SIF:

(11) Control of epigenomic landscape and development of fetal male germ cells through L-serine metabolismiScienceSeptember 20, 2024Hayashi Y, Kaneko J, Ito-Matsuoka Y, Takehara A, Funakoshi M, Maezawa S, Shirane K, Furuya S, Matsui Y.RESOURCE,SOURCE,IDENTIFIER Antibodies,Antibodies,Antibodies ,, Goat polyclonal anti-VASA,Novus Biologicals,Cat#AF2030; RRID: AB_2277369 Rabbit polyclonal anti-PHGDH,Frontier Institute,Cat#MSFR100010; RRID: AB_2571653 Mouse

(12) Cytoplasmatic Localization of Six1 in Male Testis and Spermatogonial Stem CellsInternational Journal of Stem CellsAugust 30, 2024Mingming Qin, Linzi Ma, Wenjing Du, Dingyao Chen, Guoqun Luo, Zhaoting LiuThermo Fisher Scientific). The sections were washed again and subsequentially stained with mouse anti-Vasa (AF2030; Novus Biologicals) and rabbit anti-Six1 (10709-1-AP; Proteintech Group) antibodies overnight. The samples

(13) FGF-independent MEK1/2 signalling in the developing foetal testis is essential for male germline differentiation in miceBMC BiolDecember 5, 2023Rheannon O. Blücher, Rachel S. Lim, Ellen G. Jarred, Matthew E. Ritchie, Patrick S. WesternProtein,Source and catalogue #,Species,Dilution MVH,"R&D Systems, AF2030",Goat,F: 1/100IF: 1/400 DPPA4,"R&D Systems, AF3730",Goat,F: 1/100IF: 1/400 OCT4,"Santa Cruz, sc8628",Goat,IF:

(14) Nutritional and metabolic control of germ cell fate through O‐GlcNAc regulationEMBO reportsNovember 6, 2023Yohei Hayashi, Yukiko Tando, Yumi Ito‐Matsuoka, Kaho Ikuta, Asuka Takehara, Katsutaro Morino, Hiroshi Maegawa, Yasuhisa Matsuiwere permeabilized, blocked, incubated with primary antibody (anti‐VASA, goat polyclonal, R&D systems, AF2030) overnight at 4°C and then incubated with secondary antibodies with 5 μg/ml DAPI for 2 h at 4°C. The

(15) Transcriptional metabolic reprogramming implements meiotic fate decision in mouse testicular germ cellsCell reportsJuly 25, 2023Zhang X, Liu Y, Sosa F, Gunewardena S, Crawford PA, Zielen AC, Orwig KE, Wang N.RRID:AB_10669901 SLC2A1,Abcam,Cat# ab115730; RRID:AB_10903230 gH2AX,Millpore,Cat# 05–636; RRID:AB_309864 VASA,R&D Systems,Cat# AF2030; RRID:AB_2277369 P70S6K,Cell Signaling Technology,Cat# 9234P; RRID:AB_2269803 p-70S6K,Cell Signaling Technology

(16) Efficient and scalable generation of primordial germ cells in 2D culture using basement membrane extract overlayCell Rep MethodsJune 26, 2023Overeem AW, Chang YW, Moustakas I, Roelse CM, Hillenius S, Helm TV, Schrier VFV, Gonçalves MAFV, Mei H, Freund C, et al.

(17) Efficient generation of marmoset primordial germ cell-like cells using induced pluripotent stem cellseLifeJanuary 31, 2023Seita Y, Cheng K, McCarrey JR, Yadu N, Cheeseman IH, Bagwell A, Ross CN, Santana Toro I, Yen LH, Vargas S, et al.

(18) YTHDC2 serves a distinct late role in spermatocytes during germ cell differentiationbioRxiv : the preprint server for biologyJanuary 23, 2023Bailey AS, Fuller MT.Novus Biologicals), rabbit anti-RAD51 (1:200 [N1C2], (RRID:AB_1951602), GeneTex), goat anti-VASA (1:200, AF2030, (RRID:AB_2277369), R&D Systems), mouse anti-phospho-Histone H2A.X (Ser139) (1:500 clone [JBW301], (RRID:AB_309864),

(19) Molecular phenotyping of domestic cat (Felis catus) testicular cells across postnatal development - A model for wild felidsTheriogenology WildJanuary 1, 2023Bashawat M, Braun BC, Müller K, Hermann BP.number,Working dilution cryo,Working dilution paraffin ,,,,, DDX4,Goat-polyclonal IgG,R&D systems,AF2030,1:40,- SOX9,Rabbit-monoclonal IgG,Abcam,ab185966,1:300,1:1.000 FOXO1,Rabbit-monoclonal IgG,Cell signal,2880,1:50,- UCHL1,Mouse-monoclonal

(20) Testis exposure to unopposed/elevated activin A in utero affects somatic and germ cells and alters steroid levels mimicking phthalate exposureFrontiers in endocrinologyJanuary 1, 2023Whiley PAF, Luu MCM, O'Donnell L, Handelsman DJ, Loveland KL.10X PBS stock, Gibco, Cat #70011-044) to prevent non-specific staining. All primary antibodies (DDX4, AF2030, R&D Systems, 1:400, RRID : AB_2277369; SOX9, Santa Cruz, sc-20095, 1:500, RRID : AB_661282; SMA, Sigma-Aldrich,

Write a Review

(1)

2/4/2022Anti-VASA Antibody for Immunofluorescence Yuki NagaharaThe aim of the study was to test the expression of a specific germline protein level in mouse primary tissues.
(1)

2/2/2021hVASA for Immunofluorescent Staining Yasuyo OhguchiThe aim was to identify germ cell in mouse testis. The antibody works well. Using Alexa Fluor 488 makes VASA detection. Useful for many types of imaging to mark the cell count and have a clean border ... Read Review

Human VASA Antibody

VASA Antibody

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VASA Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

R&D Systems, a Bio-Techne Brand

Citations (72)

Reviews

VASA Antibody from R&D Systems, a Bio-Techne Brand

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