R&D Systems
VASA
AF2030
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The aim of the study was to test the expression of a specific germline protein level in mouse primary tissues. VASA is an ATP-dependent DEAD-box RNA helicase found in the chromatoid body of the germ cells. This enzyme is specific to germ cells and is necessary for germ cell development.
Immunofluorescence
Testis
1:300, Overnight incubation at 4C in 2%Normal donkey serum in 0,3% triton-x100 in PBS
2%Normal donkey serum in 0,3% triton-x100 in PBS
2h incubation at RT in 2%Normal donkey serum in 0,3% triton-x100 in PBS
N/A
Confocal microscope.
Good antibody to label vasa+ cells. Tested in mouse tissue.
Works great for single stain compensation
I recommend this antibody to label vasa+ cells.