
Molecular cloning has come a long way since the first recombinant plasmids were constructed in the 1970s. Now a staple laboratory technique, cloning has undeniably enabled important developments in many biological applications, including protein expression, cell-based assays, mutagenesis, sequencing, gene editing, and more. Traditional cloning involves many sequential steps, such as DNA amplification, restriction digestion, ligation, and transformation, with additional DNA purification steps in between. Now, scientists can enjoy commercially prepared cloning and expression kits, which can offer the benefits of convenience, improved yield, greater genetic control, and more cloning options. For example, PCR cloning kits enable a simplified workflow for cloning directly from PCR products. To account for different expression systems, cloning and expression kits are also available that are specific to bacterial, mammalian, and yeast hosts. Cell-free protein expression kits are also available. Browse our catalog of cloning and expression kits from a diverse set of life science suppliers.
A myriad of advances are helping overcome current challenges.
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DELs capitalize on some of the advantages of biologics discovery
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Hold your research and proof of concept studies to the highest standards with reliable and efficient electroporation technology. Features:
Achieve high efficiency and cell viability
Rapidly transfect from 75 thousand to 700 million cells
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Set up a live demo with a Bio-Rad Imaging Specialist - Request a DemoUse the ChemiDoc MP Imaging System for flexible, high-sensitivity multiplex fluorescent and chemiluminescence western blot detection, imaging gels, analysis and documentation. This ...
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The genomic DNA isolation kit (Blood/ Cultured Cell) was used for the isolation of genomic DNA from whole blood, frozen blood, buffy coat, cultured animal and bacterial cells. The kit ensures genomic DNA with high yield and good quality from samples.
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Ideal kit for fast cloning of DNA inserts into a plasmid vector and in several plasmid constructions.
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