Spectral Flow Cytometers

Spectral flow cytometers capture the full fluorescence spectrum of labeled cells and particles, unlike conventional cytometers, which rely on optical filters to isolate specific wavelengths. Multiple specialized detectors enable the identification of multiple fluorophores simultaneously based on unique spectral fingerprints. Spectral unmixing, analogous to compensation in conventional cytometry, is used to distinguish fluorophores with even closely overlapping emission spectra. As a result, spectral cytometers can accommodate larger, more complex panels, enhancing multiparametric analysis. Selecting a spectral cytometer requires balancing cost with experimental needs, considering factors such as laser count, detector sensitivity, sample format, acquisition speed, and throughput. These specifications directly impact the resolution and efficiency of high-parameter experiments. Consult the instrument manufacturer for more specifications and features.