Oxidoreductases are a broad class of enzymes that catalyze electron transfer oxidation-reduction (redox) reactions. The diverse members of the oxidoreductase family are involved in many essential biological functions, from energy metabolism to oxidative protection and signal transduction. Enzymes like malate dehydrogenase, alcohol dehydrogenase (ADH), and pyruvate dehydrogenase (PDH), are essential for metabolic pathways such as glycolysis, fermentation, and the citric acid cycle. Peroxidases such as glutathione peroxidase (GPX) and myeloperoxidase are crucial for protecting cells from oxidative damage by reducing peroxides and superoxide radicals. Beyond their native physiological roles, these enzymes have wide-ranging applications in medicine and diagnostics, acting as biomarkers and therapeutic targets for various diseases. Biochemical assays have been developed that determine the activity of both broad and specific oxidoreductase family members. Enzyme assay kits, complete with ready-to-use reagent sets, provide a convenient and versatile toolset for protein and biomedical research.
Protein markers can offer unique advantages over other assays
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No matter the experience level, this infographic has tips for all
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The Promega GSH-Glo Glutathione Assay is used to quantify reduced glutathione in cultured cells, and it is a much faster alternative to HPLC or classic colorimetric/fluorometric thiol assays.
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The Topcells CellCount CCK-8 is used for drug cytotoxicity assays, and it performs like the well-known WST-8 kits at a more practical cost. Living cells reduce the water-soluble tetrazolium to an orange formazan that dissolves directly in the medium;...
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