Oxidoreductases are a broad class of enzymes that catalyze electron transfer oxidation-reduction (redox) reactions. The diverse members of the oxidoreductase family are involved in many essential biological functions, from energy metabolism to oxidative protection and signal transduction. Enzymes like malate dehydrogenase, alcohol dehydrogenase (ADH), and pyruvate dehydrogenase (PDH), are essential for metabolic pathways such as glycolysis, fermentation, and the citric acid cycle. Peroxidases such as glutathione peroxidase (GPX) and myeloperoxidase are crucial for protecting cells from oxidative damage by reducing peroxides and superoxide radicals. Beyond their native physiological roles, these enzymes have wide-ranging applications in medicine and diagnostics, acting as biomarkers and therapeutic targets for various diseases. Biochemical assays have been developed that determine the activity of both broad and specific oxidoreductase family members. Enzyme assay kits, complete with ready-to-use reagent sets, provide a convenient and versatile toolset for protein and biomedical research.
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CellMask Green was used to stain the plasma membrane of live primary human brain microvascular endothelial cells (HBMECs).
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In my lab, I use the Pierce BCA Protein Assay Kit to measure protein concentrations in whole-cell extracts and, occasionally, purified proteins.
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