Oxidoreductases are a broad class of enzymes that catalyze electron transfer oxidation-reduction (redox) reactions. The diverse members of the oxidoreductase family are involved in many essential biological functions, from energy metabolism to oxidative protection and signal transduction. Enzymes like malate dehydrogenase, alcohol dehydrogenase (ADH), and pyruvate dehydrogenase (PDH), are essential for metabolic pathways such as glycolysis, fermentation, and the citric acid cycle. Peroxidases such as glutathione peroxidase (GPX) and myeloperoxidase are crucial for protecting cells from oxidative damage by reducing peroxides and superoxide radicals. Beyond their native physiological roles, these enzymes have wide-ranging applications in medicine and diagnostics, acting as biomarkers and therapeutic targets for various diseases. Biochemical assays have been developed that determine the activity of both broad and specific oxidoreductase family members. Enzyme assay kits, complete with ready-to-use reagent sets, provide a convenient and versatile toolset for protein and biomedical research.
New tech improves our understanding of immune function
read more
A close look at five leading technologies
read more
We used the PureLink RNA Mini Kit to isolate total RNA from immunomagnetically purified adult mouse astrocytes for quantitative real-time PCR analysis of genes involved in mitochondrial glutamate transport and astrocyte metabolism. The kit ...
read more
We used the Lactate-Glo Assay Kit to quantify lactate concentrations in cultured primary astrocytes, mouse prefrontal cortex, cerebrospinal fluid, and serum while investigating astrocyte-specific regulation of brain energy metabolism following Npas3 ...
read more