Glutathione Assay Kits

Glutathione assay kits are available from several suppliers to determine the levels of glutathione, a major antioxidant in the body. Playing many biological roles, glutathione (GSH) serves as a cofactor for enzymes such as glutathione peroxidase, as a metabolite in the detoxification of xenobiotics, and as an antioxidant compound. The ratio of reduced glutathione (GSH) to oxidized glutathione (GSSG) can be used as a measure of oxidative stress in a cell. In fluorometric glutathione assays kits, orthophthaldehyde (OPA) reacts with GSH, which can be detected by fluorescence. Adding a reducing agent can also convert GSSG to GSH, allowing the total GSSG + GSH content to be determined. To detect GSSG alone, a GSH quencher can be used to remove GSH. Glutathione assay kits may be useful when studying the effects of oxidative stress in conditions such as cancer, diabetes and Alzheimer’s disease. Visit the glutathione assay kit suppliers for more details, including kit components.