Nuclease-Free Water from Promega

Product Specs
ItemNuclease-Free Water
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberP1193
Quantity50ml

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Reconstructing the genetic ancestry of the archaeological site in Amiternum: From Roman Imperial to early medieval times in Central ItalyiScienceNovember 20, 2026Elisabetta Moroni, Vincenzo Agostini, Dario Ciabini, Giulia Colombo, Rosalinda Di Gerlando, Nicola Rambaldi Migliore, Osvaldo Zarivi, Ornella Semino, Alessandro Achilli, Alfonso Forgione, et al.proteins" ,, Isopropanol,Carlo Erba,415154 Bleach 100%,Aurogene,AU1125 Nuclease-free water,Promega,P1193 EDTA solution pH 8.0 (0.5 M) for molecular biology,PanReach Applichem ITW Reagents,"A4892,0500" Proteinase

(2) Single-cell epigenetic and transcriptomic states across the continuum of monoclonal B cell lymphocytosis to chronic lymphocytic leukemiaGenome BiologyApril 15, 2026Anja C. Rathgeber, Stacey M. Fernandes, Adi Nagler, Shuqiang Li, David M. Dorfman, Lars Bullinger, Matthew S. Davids, Jennifer R. Brown, Kenneth J. Livak, Leif S. Ludwig, et al.(Sigma, Cat. no. 74385)100 µl BSA 10% (Sigma, Cat. no. A3059)875 µl Nuclease-free H2O (Promega, Cat. no. P1193) Washing buffer (10 ml stock) 100 µl 1 M Tris–HCl (pH 7.4) (Sigma, Cat. no. T2194)20 µl 5 M NaCl (Santa

(3) Multiplex PCR for differentiating Ornithodoros tick vectors in South Africa: Proof of conceptOnderstepoort Journal of Veterinary ResearchOctober 27, 2025Susan West, Juanita van Emmenes, Carin BoshoffµL of 0.5 µM 16S-RArg (retinoic acid receptor gamma) primer, 15 µL of nuclease-free water (Promega P1193, US) and 5 µL of DNA template. In the negative control reaction, nuclease-free water was used instead

(4) Caffeic Acid Inhibits Degranulation, Cytokine Secretions, and IP3 Receptor 2 Gene Expression in Compound 48/80-Stimulated Mouse P815 Mast CellsInternational journal of molecular sciencesOctober 16, 2025Chang KC, Lin JY.the prepared cDNA samples were adjusted to the same concentration with nuclease-free water (Promega, P1193, Madison, WI, USA), respectively. The primers of target genes in mouse P815 mast cells were devised

(5) Efficient CRISPR/Cas9‐mediated knockin of reporter genes in rats at ROSA26 by pronuclear microinjectionDevelopment, Growth & DifferentiationApril 23, 2025Takaya Abe, Ken‐ichi Inoue, Hiroshi Kiyonarisolution: resuspend at 1 μg/μL in nuclease‐free water and store at −80°C.Nuclease‐free water (Promega, USA, P1193).Cas9 protein (ThermoFisher Scientific, A36497).Donor vector: dilute at 10 ng (or 100 ng)/μL in nuclease‐free

(6) Mutations to transcription factor MAX allosterically increase DNA selectivity by altering folding and binding pathwaysNat CommunJanuary 14, 2025Renee Hastings, Arjun K. Aditham, Nicole DelRosso, Peter H. Suzuki, Polly M. FordyceThen, we added 1.5 µL of recombinant RNAsin (Promega N2515) and 6 µL of nuclease-free water (Promega P1193) and mixed by pipette until no phase separation was visible. We introduced PURExpress onto the device

(7) Pathobiont-triggered induction of epithelial IDO1 drives regional susceptibility to Inflammatory Bowel DiseasebioRxiv : the preprint server for biologyJanuary 4, 2025Spencer PN, Wang J, Smith EP, Spiga L, Simmons AJ, Kim T, Kim W, Brown ME, Yang Y, Kaur H, et al.Prolong Gold AntiFade Reagent,Thermo Fisher Scientific,P36930 Glycerol,Invitrogen,15514-011 "Water, Nuclease Free",Promega,P1193 Quick-Load® Purple 1 kb Plus DNA Ladder,New England Bio Labs,N0550S 0.5M EDTA pH 8,Corning,46-034-CI 1M

(8) Targeting cancer stem cells by TPA leads to inhibition of refractory sarcoma and extended overall survivalMolecular Therapy: OncologyDecember 19, 2024Karina Galoian, Daniel Bilbao, Carina Denny, Natalia Campos Gallego, Evan Roberts, Daniel Martinez, H.T. Templesamples were centrifuged, and the RNA pellet was dried and then dissolved in 20 μL RNase-free water (P1193, Promega). RNA was quantified, and the quality of RNA was analyzed using NanoDrop (ND-1000, Thermo Fisher Scientific

(9) Analysis of translatomic changes in the Ubqln2P497S model of ALS reveals that motor neurons express muscle-associated genes in non-disease statesFrontiers in NeurologyNovember 19, 2024Wesley M. Stansberry, Natalie C. Fiur, Melissa M. Robins, Brian A. Pierchalaand western blotting Precipitated IP protein pellets were re-suspended in 100 μl of molecular grade water (Promega, # PR-P1193). 100 μl of 2X sodium dodecyl sulfate (SDS) sample buffer (4% SDS, 20% glycerol, 1% β-mercaptoethanol

(10) Preparation of Frozen Non-Human Primate Fetal Islets for Combined Single Nuclei RNA-Sequencing and ATAC-Sequencing, and Bulk MetabolomicsJournal of Visualized ExperimentsNovember 8, 2024Darian T. Carroll, Sarah R. Lindsley, Melissa Kirigiti, Alex Buko, Angela Jones, Paul Kievit, Maureen Gannon20,Fisher Bioreagents,BP337–500,Prepare a 20% stock using MilliQ water Nuclease-Free Water,Promega,PR-P1193, DNA LoBind Tubes (5 mL),Eppendorf,30108310, Cryovials (1.5 mL),VWR,20170–225, Posi-Click Tubes (0.6

(11) A study on viruses and bacteria with particular interest on Mycoplasma pneumoniae in children with exacerbation of asthma from a tertiary care hospital in Sri LankaAccess MicrobiologyJuly 30, 2024Lakmini Inoka Wijesooriya, Victoria Chalker, Priyantha Perera, N. P. Sunil-Chandracontrol [PCR-grade water: water, nuclease-free, 50 mL (2×25 mL) from Promega, USA, catalogue number: P1193]. The products of the second PCR were separated using gel electrophoresis. Once the positive bands for

(12) Protocol for the generation and purification of high-molecular-weight covalent RNA-DNA hybrids with T4 RNA ligaseSTAR ProtocMarch 15, 2024Oscar Laguía, Giuseppe Bosso, Jorge Martínez-Torrecuadrada, Samuel Míguez-Amil, Rafael Fernández-Leiro, Maria A. Blascokit,NEB,M0204S Monarch RNA cleanup kit (10 μg),NEB,T2030S DMSO,Sigma,D2650 Nuclease-free water,Promega,P1193 TRIS-HCl pH 7.5,Alaos,TR085C TRIS-HCl pH 8,Alaos,TR095C NaCl,Alaos,CS131C 40% bis acrylamide/bis solution 19:1

(13) Elucidation of GPR55-Associated Signaling behind THC and LPI Reducing Effects on Ki67-Immunoreactive Nuclei in Patient-Derived Glioblastoma CellsCellsNovember 17, 2023Kolbe MR, Hohmann T, Hohmann U, Maronde E, Golbik R, Prell J, Illert J, Strauss C, Dehghani F.(Biotium, Fremont, CA, USA, 31000), and 4.75 µL of nuclease-free water (Promega Inc., Madison, WI, USA, P1193) The PCR reaction was performed for 40 cycles using a PCR cycler (7900HT Fast Real-Time PCR System,

(14) Selection of Molecules with Immunological Potential from Excretory and Secretory Products from the Nematode Haemonchus placei by Cell Proliferation and Gene Expression AssaysBio ProtocJune 20, 2023Jocelyn Maza-Lopez, Rene Camas-Pereyra, María Eugenia López-Arellano, Carla O. Contreras-Ochoa9180)Isopropyl alcohol (Sigma-Aldrich, catalog number: W292907)Nuclease-free water (Promega, catalog number: P1193)Agarose (Bio-Rad, catalog number: 1613100)Ethidium bromide 10 mg/mL (Bio-Rad, catalog number: 1610433)RQ1 RNase-Free DNase

(15) Selection of Molecules with Immunological Potential from Excretory and Secretory Products from the Nematode Haemonchus placei by Cell Proliferation and Gene Expression AssaysBIO-PROTOCOLJune 20, 2023Jocelyn Maza-Lopez, René Camas-Pereyra, María Eugenia López-Arellano, Carla O. Contreras-Ochoa9180)Isopropyl alcohol (Sigma-Aldrich, catalog number: W292907)Nuclease-free water (Promega, catalog number: P1193)Agarose (Bio-Rad, catalog number: 1613100)Ethidium bromide 10 mg/mL (Bio-Rad, catalog number: 1610433)RQ1 RNase-Free DNase

(16) The Occurrence of Folate Biosynthesis Genes in Lactic Acid Bacteria from Different SourcesFood Technology and BiotechnologyJune 1, 2023Fenny Amilia Mahara, Lilis Nuraida, Hanifah Nuryani Lioe, Siti NurjanahµM of each forward and reverse primer, 1 µL DNA template (>150 ng) and 7 µL nuclease-free water (NFW P1193; Promega Corporation). The amplifications were carried out using an Applied Biosystems 2720 Thermal Cycler

(17) Implications of differential transcription start site selection on chronic myeloid leukemia and prostate cancer cell protein expressioniScienceDecember 22, 2022Surani AA, Spriggs KA, Ufer C, Polytarchou C, Montiel-Duarte C.Scientific,Cat#10644795 2-Propanol for molecular biology,Sigma-Aldrich,Cat#278475 Nuclease free water,Promega,Cat#P1193 "MG132, proteasome inhibitor",Sigma-Aldrich,Cat#474790;CAS:133407-82-6 "Bortezomib, proteasome inhibitor",Santa Cruz

(18) Use of a Bacterial Artificial Chromosome to Generate Recombinant SARS-CoV-2 Expressing Robust Levels of Reporter GenesMicrobiology SpectrumDecember 21, 2022Chengjin Ye, Luis Martinez-SobridoNEB buffer set (New England Biolabs, catalog no. B7200S), nuclease-free water (Promega, catalog no. P1193), Opti-MEM (Thermo Fisher Scientific, catalog no. 31985-070), PacI (New England Biolabs, catalog no.

(19) Silencing of hypothalamic FGF11 prevents diet-induced obesityMolecular BrainSeptember 5, 2022Jae Hyun Cho, Kyungchan Kim, Han Chae Cho, Jaemeun Lee, Eun-Kyoung KimTrizol reagent (Invitrogen, 15596018). The RNA pellet was dissolved in nuclease-free water (Promega, P1193) and total RNA concentration was determined using a NanoDrop spectrophotometer (DeNovix, DS-11). Total

(20) The Use of Precision-Cut Lung Slices for Studying Innate Immunity to Viral InfectionsCurr ProtocAugust 1, 2022Michalaki C, Dean C, Johansson C.no. 15596018)ChloroformRNeasy Mini kit (Qiagen, cat. no. 74106)Nuclease‐free water (Promega, cat. no. P1193)High‐Capacity RNA‐to‐cDNA kit (Thermo Fisher, cat. no. 4387406)Quantitect Probe PCR Master Mix (Qiagen,

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