HRP Substrates

Horseradish peroxidase (HRP) substrates are used in the immunostaining and immunodetection of specific proteins and antigens. HRP is a widely used label, often conjugated to secondary antibodies in immunochemical applications such as immunohistochemistry, western blotting, and ELISA. Common HRP substrates include: ABTS, AEC, DAB, ECL, TMB, and Amplex Red. When choosing a HRP substrate, consider the desired colors manifested in the final staining to ensure proper differentiation.