TMB Substrate Set from BioLegend

Product Specs
ItemTMB Substrate Set
CompanyBioLegend
Price $42.00Supplier Page View Company Product Page
Catalog Number421101
ApplicationsELISA
Quantity110 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) RNA binding protein Pcbp1 maintains mitochondria integrity to promote antibody production and germinal center responseSci AdvApril 10, 2026Lizhen Zhu, Li Lin, Tuan Qi, Geng Li, Zhixing Liang, Jiancheng Yu, Yifan Fu, Yue Peng, Xing Changincubation. For development, plates were incubated with trimethylboron (TMB) substrate (BioLegend, 421101) at room temperature in the dark for 1 to 5 min. The reaction was stopped with equal volume of 1 M sulfuric

(2) The therapeutic effects of the VEGF decoy receptor fusion protein VEGF-Grab in chronic inflammatory diseasesEBioMedicineMarch 19, 2026Youngeun Lee, Hee-Young Lee, Hye Seong Lim, Jin A. Shin, Hyun Gul Yang, Saseong Lee, Grazia Mercadante, Valeria Cicatiello, Sandro De Falco, Hyungmin Kim, et al.p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer, Sigma–Aldrich) or TMB substrate solution (catalog 421101; BioLegend) was added and incubated at RT for colour development. When sufficient colour development

(3) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernthe addition of 50 µl of TMB substrate prepared as per the manufacturer’s instructions (BioLegend, 421101). The wells were visually monitored for color change and the ELISA was stopped by the addition of 50

(4) Generation of Aptamers for the Selective Detection and Neutralization of soluble Lymphotoxin alphaMolecular Therapy Nucleic AcidsMarch 12, 2026Matthew Stephens, Eman Nassef, Pierre-Yves von der von der Weidlight. Wells were washed again 3× with PBST before being incubated with TMB substrate (Biolegend Cat No. 421101) for 30 min in the dark. Th reaction was halted by addition of 0.1 M HCL, and 450 nm absorbance was

(5) All‐PEG‐Like Block Copolymers Self‐Assemble into Stealth Nanocarriers for Drug DeliveryAdvanced ScienceMarch 1, 2026Parul Sirohi, Brooke E. Silverstein, Yulia Shmidov, Sonal Deshpande, Joy Tong, Yvonne Y. Ma, Chinmay S. Potnis, Soumen Saha, Xinghai Li, Max R. Ney, et al.developed with 100 µL of pre‐mixed 3,3′,5,5′‐tetramethylbenzidine (TMB) liquid substrate (BioLegend # 421101) for 10 min at room temperature before stopping the reaction with the addition of 100 µL of Stop Solution

(6) IL‐2 silencing enables Tfh cell expansion during vaccination but is redundant for antibody productionImmunology & Cell BiologyFebruary 24, 2026Silvia Innocentin, Ross McKenzie, Jayalini Assalaarachchi, Helena A Carslaw, Anusha Gupta, Sanne Cole, Adrian Liston, Michelle A Linterman, Louise Webb, Alice R Burton(#ab97230, Abcam) antibody for 1 h at RT. In all cases, plates were developed with TMB substrate set (#421101, BioLegend) and the reaction stopped with 2.5 m H2SO4. Absorbance was measured with PHERAstar FS plate

(7) Recombinant Dabie bandavirus as a bivalent vaccine platform inducing protective immunity against intracellular pathogens and cancerMolecular TherapyFebruary 4, 2026Hyo-Jin Ro, Yebeen Lee, Kyeongseok Jeon, Yujin Kim, Seung Ho Baek, Green Kim, Joowan Kim, Jun-Gu Kang, Na-Yoon Jang, Si-Hyeon Lee, et al.#NB7215). After incubation, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate solution (BioLegend #421101) was added, and color development was allowed to proceed for 7 min. The reaction was stopped by adding

(8) Polymer-lipid hybrid nanoparticle enhances mRNA delivery and T cell-mediated immunitybioRxivJanuary 29, 2026Xiaolei Cai, Min Chen, Guoshuai Cao, Nicholas Asby, Derek Elli, Haley Gula, Vlad Nicolaescu, Duy-Thuc Nguyen, Xiaodan Huang, Tanushree Dangi, et al.plates were washed gently 5 times using 200 μL 1×PBS+0.05% Tween 20. TMB substrate (100 μl, BioLegend #421101) were further added into each well and incubate for 20 min under dark. Finally, 100 μL stop reagent

(9) Functional Convergence of Genetically Diverse B-Cell Receptors in Simian-HIV Infected Rhesus MacaquesbioRxivJanuary 24, 2026Shengli Song, Hui Li, Chen-Hao Yeh, Akiko Watanabe, Masayuki Kuraoka, Hongmei Gao, Xiaoying Shen, Celia C. LaBranche, Wilton B. Williams, Kevin O. Saunders, et al.temperature for 1 hour in the dark. Following another three washes, TMB substrate (BioLegend, Cat# 421101) was added and incubated at room temperature in the dark for 10 minutes for RM samples and 5 minutes

(10) Profiling Allogeneic HLA-specific B-cell Responses Utilizing a 64-plex Single-HLA Reporter Cell PanelbioRxivJanuary 23, 2026Shengli Song, Emma F. King, Ashley M. Drabik, Jean Kwun, Cliburn Chan, Annette M. Jackson, Garnett Kelsoe, Stuart J. KnechtleIgλ (Southern Biotech, 2071–05) Abs was added. After three further washes, TMB substrate (BioLegend, 421101) was added and incubated at room temperature in the dark for 5 min. The reaction was stopped by addition

(11) Enhancing anti-tumor immunity through co-blocking PD-L1 and TIGIT by facilitating tumor-directed responses and additional VEGF inhibitionFrontiers in ImmunologyJanuary 14, 2026Xi Zhu, Xiaopei Cui, Haijia Yu, Jingen Xu, Xiaofang Chen, Xiaochen Ren, Xiaoyue Wei, Shi Chen, Yangtin Wang, Liyang Fei, et al.streptavidin-HRP (CST, Cat#: 3999S), and plates were washed six times. TMB substrate (BioLegend, Cat#: 421101) was added, followed by stop solution. Absorbance was measured at 450 nm (OD450). Deletion of PD-L1

(12) Patients Suffering From Post‐ COVID ‐19 Syndrome Feature Enhanced Antibody Reactivity Towards Specific Linear Epitopes Within EBV EBNA1Scandinavian Journal of ImmunologyJanuary 10, 2026Peter Lorenz, Felix Steinbeck, Florian Fricke, Franz Mai, Wendy Bergmann‐Ewert, Christine Wossidlo, Emil C. Reisinger, Brigitte Müller‐Hilkeantibodies conjugated to peroxidase (Jackson Dianova 109‐035‐088) and subsequent TMB substrate (BioLegend 421101). After stopping the 20 min substrate reaction with 2 N sulfuric acid, the OD450nm values were recorded

(13) Broadening SARS-CoV-2 Immunity by Combining ORFV and Protein-Based VaccinesVaccines (Basel)January 4, 2026Alena Reguzova, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf Amann(1:1000, Abcam, ab97245). Following 1 h incubation, plates were developed using TMB substrate (Cat. No. 421101, BioLegend) and the reaction was stopped after sufficient color development by adding stop solution

(14) Mother and child stool bacteriomes 1–2 years postpartum: associations with maternal history of gestational diabetes and child atopic dermatitisiScienceDecember 19, 2025Kristi Alnek, Anu Bärenson, Oliver Aasmets, Helis Janson, Ija Talja, Brita Laht, Tamara Vorobjova, Anne Kirss, Ondrej Cinek, Raivo Uibo, et al.milk powder,AppliChem,Cat#A0830 "3,3',5,5'-tetramethylbenzidine (TMB) Substrate Set",BioLegend,Cat#421101 Gliadin from wheat,Sigma-Aldrich,Cat#9007-90-3 Bovine-lactoglobulin,Sigma-Aldrich,Cat#L3908 o-phenylenediamine

(15) The dietary phytochemicals carnosic acid and sulforaphane regulate inflammatory markers in ulcerative colitis patient-derived colonoidsFront PharmacolDecember 18, 2025Rocío Rivera Rodríguez, Siri Sæterstad, Ann-Therese Chattergoon Ali, Linn-Karina M. Selvik, Ingunn Bakke, Torunn Bruland, Jeremy J. Johnson(Lipocalin-2/NGAL). Assay development was performed with the 3,3′,5,5′tetramethylbenzidine (TMB) substrate set (421101, BioLegend®) and the reaction stopped with 2N sulfuric acid (231-639-5, VWR). Absorbance was read at

(16) Heterologous ORFV–Ad26 vaccination broadens antibody breadth and amplifies cellular immunity against SARS-CoV-2 spikeFrontiers in ImmunologyNovember 18, 2025Alena Reguzova, Verena Haug, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf Amann(1:1000, Abcam, ab97245). Following 1-hour incubation, plates were developed using TMB substrate (Cat. No. 421101, BioLegend) and the reaction was stopped after sufficient color development by adding stop solution

(17) Discovery and molecular mechanism of potent neutralizing antibody from humanized mice with respiratory syncytial virusPLOS PathogensNovember 17, 2025Zheng Zhang, Rui Feng, Long Zhang, Qi Yang, Xuehua Chen, Xiaoxiao Wang, Cui Nie, Wei Peng, Xiangxi Wang, Ling Zhu, et al.well, followed by incubation at 37 °C for 1 hour. The substrate tetramethylbenzidine (TMB) (BioLegend, 421101) was added at a volume of 50 μL per well and incubated at room temperature in the dark for approximately

(18) Heterologous prime-boost vaccination with VLA2001 and an ORFV-based vector enhances spike- and nucleocapsid-specific immunity in miceFrontiers in ImmunologySeptember 18, 2025Alena Reguzova, Verena Haug, Carina Metz, Melanie Müller, Madeleine Fandrich, Alex Dulovic, Ralf AmannAbcam, ab97245). Following a 1-hour incubation, plates were developed using TMB substrate (Cat. No. 421101, BioLegend) and the reaction was stopped after sufficient color development by adding stop solution

(19) Germinal center formation is resilient to CD69 deletion on T follicular helper cellsImmunology & Cell BiologyAugust 4, 2025Stephane M Guillaume, Helena A Carslaw, Silvia Innocentin, Louise M C Webb, Adrian Liston, William S Foster, Michelle A Lintermanhour at RT. Plates were developed using 50 μL tetramethylbenzidine (TMB) substrate kit (BioLegend, #421101) until a good dynamic range was observed, at which point the reaction was quenched using 50 μL 0.1 M

(20) ST8Sia6 overexpression protects pancreatic β cells from spontaneous autoimmune diabetes in nonobese diabetic miceJ Clin InvestAugust 1, 2025Justin Choe, Paul Belmonte, Sydney Crotts, Thanh Nguyen, David Friedman, Alexi Zastrow, Matthew Rajcula, Brady Hammer, Claire Wilhelm, Michael J. Shapiro, et al.were washed 10 times with PBS plus 0.005% Tween-20. One hundred microliters of substrate (BioLegend 421101) was added to each well and incubated at room temperature for 10 minutes; then the reaction was halted

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TMB Substrate Set from BioLegend

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TMB Substrate Set from BioLegend

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