TMB Substrate Set from BioLegend

Product Specs
ItemTMB Substrate Set
CompanyBioLegend
Price $42.00Supplier Page View Company Product Page
Catalog Number421101
ApplicationsELISA
Quantity110ML

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Innate immune recognition of Debaryomyces hansenii requires Dectin-1-Card9 signalingInfection and ImmunitySeptember 8, 2026Kevin P. Newhall, Sarah K. McNeer, Scott T. Espenschied, Elora G. Dolan, Julie Y. Zhou, Deborah A. Hogan, Thaddeus S. StappenbeckMouse Tnf was detected per manufacturer’s directions (BioLegend #430901). TMB substrate (BioLegend 421101) was read on a BioTek Cytation5 plate reader measuring OD450 with OD570 background subtraction. Supplementary

(2) A Rapid Poly(ethylene glycol)-Assisted Magnetic Isolation Approach for High-Throughput Extracellular Vesicle Isolation and Subsequent Biomarker AnalysisACS NanoSeptember 8, 2026Li Sun, Leanne Duke, Cynthia Vied, Grace Hammel, Brandon Pate, Julia Wang, Yanping Zhang, Dai-Shi Tian, Jianqing Fan, Yi RenTween-20 in PBS) to remove any unbonded antibody. A total of 100 μL of TMB substrate solution (BioLegend, 421101) was developed for 10 min in the dark or until it reached the desired color. After stopping with 50

(3) Rapid discovery of monoclonal antibodies via high-throughput single BCR affinity sequencingNucleic Acids ResAugust 20, 2026Mengzhu Hu, Qiuyu Lian, Xiaonan Cui, Xiao Chen, Xue Dong, Mengge Huang, Guangchao Wang, Yan Hu, Hao Zhang, Jiacan Su, et al.at 37°C. Plates were washed four times with PBS-T and developed by adding TMB substrate (Biolegend, 421101) to each well. The plates were incubated at room temperature for 15 min, and 2 N sulfuric acid was added

(4) Stage-Dependent Transmural Redistribution of NET-Associated Structures and Altered DNA Architecture in Acute AppendicitisCellsAugust 19, 2026Jonas Pachmann, Malik Szep, Pia Meyer Zu Himmern, Max Rickes, Sara Al-Madhi, Mihailo Andric, Mirhasan Rahimli, Jessica Stockheim, Katrin Hippe, Franziska S. Karras, et al.room temperature. Plates were washed three times with PBS-T, followed by addition of TMB substrate (421101, BioLegend, San Diego, CA, USA; 50 µL/well) and incubation for 45 min in the dark. We stopped the reaction

(5) Expression and Role of the RNA-Binding Protein, ZFP36L1, in Mouse T Follicular Helper Cell Differentiation and FunctionEur J ImmunolAugust 18, 2026Helena A. Carslaw, Sam Woolliscroft, Emily M. Watson, Sarah E. Bell, Michelle A. Linterman, Martin Turner, Louise M. C. Webb50 µL anti‐mouse IgG1‐HRP (1/8000, ab97240, Abcam) for 1 h at RT, followed by 50 µL TMB substrate (421101, BioLegend) until an appropriate dynamic range was reached. The reaction was quenched by addition of

(6) Dynamic Arrest and Tunable Gelation of Immunogenically Inert POEGMA NanoparticlesACS Polymers AuAugust 12, 2026Parul Sirohi, Max R. Ney, Yulia Shmidov, Brooke E. Silverstein, Anurag Singh, Gable Wadsworth, Sonal Deshpande, Yvonne Y. Ma, Lixin Fan, Priya R. Banerjee, et al.again and developed using 100 μL of premixed 3,3′,5,5′-tetramethylbenzidine (TMB) substrate (BioLegend #421101) for 10 min at room temperature. The reaction was stopped with 100 μL of stop solution (BioLegend #423001),

(7) Anti-CD20 Therapies and Antinephrin Autoantibodies in IgA Nephropathy With Minimal Change DiseaseKidney International ReportsAugust 1, 2026Yu Liang, Zerui Yang, Xiaohan Yuan, Qian Jin, Huixian Li, Yihui Zhang, Li Jin, Songxia Quan, Linpei Zhang, Li Liu, et al.the detection of samples and standards, respectively. Finally, 50 μl/well TMB substrate (BioLegend, 421101) was added with incubation for 20 minutes and stopped with 50 μl/well 0.2M H2SO4. Plasma samples with

(8) UFL1-Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer ProgressionAdv Sci (Weinh)July 29, 2026Xiuqing Ma, Rui Wan, Yueyuan Zhong, Ziyang Cui, Xuan Zhang, Xiaoqiang He, Rong Wang, Lei Huang, Wan-Yang Sun, Rong-Rong He, et al.antibody for 1 h at room temperature. Subsequently, 3,3’,5,5’‐Tetramethylbenzidine (TMB; BioLegend, 421101) was added. The TMB reaction was then stopped using Stop Solution (BioLegend, 423001), and the absorbance

(9) JN.1-adapted vaccination is associated with readjustment of ancestral memory B cells toward neutralization within the JN.1 antigenic spaceNat CommunJuly 24, 2026Metodi V. Stankov, Matthias Bruhn, Markus Hoffmann, Abdus Salam, Amy Eichmann, Inga Nehlmeier, Luis A. Manthey, Torsten Witte, Stefan Pöhlmann, Gerrit Ahrenstorf, et al.temperature followed by three washing steps. ELISAs were developed using TMB Substrate Set (BioLegend, Cat # 421101) for 30 min, reaction stopped with Stop Solution (BioLegend, Cat # 423001) and absorbance measured at

(10) Mucin degradation by Akkermansia muciniphila promotes Alistipes-dependent tryptophan metabolism and Th17-driven autoimmunityMucosal ImmunologyJuly 4, 2026Xun Lin, Ankita Singh, Xindi Shan, Suzanne Tawch, Isabel Sakarin, Tej Bahadur, Nancy McLinskey, Patricia Melville, Bettina C. Fries, Patricia K. Coyle, et al.anti-human IgG-HRP (SouthernBiotech, cat#:2040–05, 1:5000). After washing, TMB substrate (Biolegend, cat#: 421101) was added, the reaction was stopped, and absorbance was measured at 450 nm and 570 nm using spectrometer. LBP

(11) Induction of multiple HIV-1 neutralizing B Cell Precursors in HumansmedRxivJune 30, 2026Chen-Hao Yeh, Stephen R. Walsh, Ruth J. Parsons, Qianyi E. Zhang, Bhishem Thakur, Kijun Song, Kejun Liu, Helene Fradin Kirshner, Elizabeth Van ltallie, Matthew Clark, et al.temperature in the dark. Plates were washed three times, developed with TMB substrate (BioLegend, Cat#421101) for 5 min at room temperature in the dark, and stopped with 1 M H3PO4 (Sigma-Aldrich). Absorbance was

(12) Real-world longitudinal assessment of anifrolumab in patients with systemic lupus erythematosus: clinical outcomes, safety and modulation of cytokines and neutrophil activityRMD OpenJune 28, 2026Alp Temiz, Rita Noversa de Sousa, Janina Schoen, Clara Reichardt, Kathrin Standfest, Andreas Wirsching, Melanie Hagen, Giulia Corte, Marco Muñoz Becerra, Koray Tascilar, et al.After 90 min incubation, plates were washed three times with PBS-T before adding 50 µL TMB substrate (#421101, BioLegend). The reaction was stopped after 60 min by adding 25% H2SO4 and measured at 450 nm with wavelength

(13) B cell TET2 recruits OGT for nuclear TET2 and H2B O-GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody responseCell ReportsJune 23, 2026Shili Li, Yibo Peng, Zhimin Yang, Xin Li, Zhenming Xu, Carlos E. Rivera, Paolo Casalidevelopment with O-phenylenediamine (OPD, P8806-50TAB, Sigma-Aldrich) or 3,3′,5,5′-Tetramethylbenzidine (TMB, 421101, BioLegend) substrate and measurement of converted substrate absorbance at 492 nm or 450 nm, respectively.

(14) Anti-CD38/Anti-CD20 Rescue Therapy for Post-Transplant Recurrent Focal Segmental GlomerulosclerosisKidney International ReportsJune 16, 2026Juliette Leon, Elise Ducrocq, Marie-Camille Lafargue, Dominique Bertrand, Lucie Maigret, Mohamad Zaidan, Arthur Michon Colin, Eric Thervet, Antoine Durrbach, Nassim Kamar, et al.room temperature with shaking (500 rpm). Following 5 additional washes, 100 μl/well of TMB substrate (421101, BioLegend) was added and allowed to react for 5 minutes at room temperature. The reaction was stopped

(15) HnRNP A1 and A2B1 enforce Ezh2 mRNA splicing to promote germinal center B cell responsesNature CommunicationsJune 11, 2026Zhijian Zhu, Rui Zhang, Haoran Kang, Meiyuan Chen, Hengjun Huang, Jing Wang, Ling Guo, Yuxing Li, Wenjing Chen, Limin Meng, et al.temperature, followed by three washes with PBST. The signal was developed using a TMB substrate (BioLegend, 421101), and the optical density at 450 nm (OD450) was measured using a microplate reader. Data analysis was

(16) Seasonal influenza mRNA vaccine induces stronger innate and comparable or better adaptive responses than licensed inactivated vaccinesNPJ VaccinesMay 26, 2026Erick Bermúdez-Méndez, Klara Lenart, Rodrigo Arcoverde Cerveira, Olivia Engstrand, Fredrika Hellgren, Alberto Cagigi, Sebastian Ols, Edith Jasny, Annika Reinhardt, Benjamin Petsch, et al.automatic plate washer. Signal was developed upon addition of TMB substrate solution (BioLegend, cat. no. 421101). The reaction was stopped after 7 min by addition of 1 M H2SO4. Absorbance was measured at 450 nm with

(17) Peroxisomal ABCD1 deficiency in mice drives Th1 bias through 25-HC–LXR signaling in CD4+ T cellsFrontiers in ImmunologyMay 20, 2026Reina Maeda, Mayu Oishi, Ryutaro Nagamori, Mari Hikosaka-Kuniishi, Shiro Watanabe, Jung-Bum Lee, Masashi Morita, Takanori Sodetection antibody. HRP-conjugated streptavidin (405210), and 3,3’,5,5’-tetramethylbenzidine substrate (421101) were also obtained from BioLegend. T cell restimulation assay Abcd1-deficient mice and wild-type

(18) Interleukin-4-producing type 2 innate lymphoid cells in the lymph node promote proallergic Tfh13 cell differentiationImmunityMay 19, 2026DongUk Lee, Meng-Ping Lu, Priya Rajamanimuthu, Mrinmoy Das, Enxhi Ferraj, Courtney Fisher, Peri Matatia, Chun-Wei Chen, Raif S. Geha, Caroline L. Sokol, et al.,Thermo Fisher Scientific,Cat# C34571 Fluoromount-G,Southern Biotech,Cat# 0100-01 TMB Substrate Set,Biolegend,Cat# 421101 MMCP-1 ELISA Kit,Thermo Fisher Scientific,Cat# 88-7503-22 Experimental models: Organisms/strains,Experimental models

(19) RNA binding protein Pcbp1 maintains mitochondria integrity to promote antibody production and germinal center responseSci AdvApril 10, 2026Lizhen Zhu, Li Lin, Tuan Qi, Geng Li, Zhixing Liang, Jiancheng Yu, Yifan Fu, Yue Peng, Xing Changincubation. For development, plates were incubated with trimethylboron (TMB) substrate (BioLegend, 421101) at room temperature in the dark for 1 to 5 min. The reaction was stopped with equal volume of 1 M sulfuric

(20) Identification of ovarian cancer-associated antigens forming complexes with autoantibodiesJournal of Ovarian ResearchApril 7, 2026Mimori Fujimori, Kai Uryu, Makoto Kobayashi, Kotaro Sugimoto, Yuta Endo, Shigenori Furukawa, Shu Soeda, Yoshio Kodera, Keiya Fujimori, Hideki Chibafor 1 h at 37℃. Signal development was performed using 3,3’,5,5’ tetramethyl benzidine substrate kit (421101, Biolegend, San Diego, CA). Absorbance at 490 nm was measured by Microplate Reader (AS ONE, Osaka, Japan)

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TMB Substrate Set from BioLegend

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TMB Substrate Set from BioLegend

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