TMB Substrate Set from BioLegend

Product Specs
ItemTMB Substrate Set
CompanyBioLegend
Price $42.00Supplier Page View Company Product Page
Catalog Number421101
ApplicationsELISA
Quantity110 mL

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BioLegend

8999 BioLegend Way

San Diego, California 92121

United States

Phone: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587

Company Profile

Website: biolegend.com

(1) Anti-CD20 Therapies and Antinephrin Autoantibodies in IgA Nephropathy With Minimal Change DiseaseKidney International ReportsAugust 1, 2026Yu Liang, Zerui Yang, Xiaohan Yuan, Qian Jin, Huixian Li, Yihui Zhang, Li Jin, Songxia Quan, Linpei Zhang, Li Liu, et al.the detection of samples and standards, respectively. Finally, 50 μl/well TMB substrate (BioLegend, 421101) was added with incubation for 20 minutes and stopped with 50 μl/well 0.2M H2SO4. Plasma samples with

(2) UFL1-Mediated UFMylation of ENO1 Restrains Aerobic Glycolysis and Colorectal Cancer ProgressionAdv Sci (Weinh)July 29, 2026Xiuqing Ma, Rui Wan, Yueyuan Zhong, Ziyang Cui, Xuan Zhang, Xiaoqiang He, Rong Wang, Lei Huang, Wan-Yang Sun, Rong-Rong He, et al.antibody for 1 h at room temperature. Subsequently, 3,3’,5,5’‐Tetramethylbenzidine (TMB; BioLegend, 421101) was added. The TMB reaction was then stopped using Stop Solution (BioLegend, 423001), and the absorbance

(3) JN.1-adapted vaccination is associated with readjustment of ancestral memory B cells toward neutralization within the JN.1 antigenic spaceNat CommunJuly 24, 2026Metodi V. Stankov, Matthias Bruhn, Markus Hoffmann, Abdus Salam, Amy Eichmann, Inga Nehlmeier, Luis A. Manthey, Torsten Witte, Stefan Pöhlmann, Gerrit Ahrenstorf, et al.temperature followed by three washing steps. ELISAs were developed using TMB Substrate Set (BioLegend, Cat # 421101) for 30 min, reaction stopped with Stop Solution (BioLegend, Cat # 423001) and absorbance measured at

(4) Induction of multiple HIV-1 neutralizing B Cell Precursors in HumansmedRxivJune 30, 2026Chen-Hao Yeh, Stephen R. Walsh, Ruth J. Parsons, Qianyi E. Zhang, Bhishem Thakur, Kijun Song, Kejun Liu, Helene Fradin Kirshner, Elizabeth Van ltallie, Matthew Clark, et al.temperature in the dark. Plates were washed three times, developed with TMB substrate (BioLegend, Cat#421101) for 5 min at room temperature in the dark, and stopped with 1 M H3PO4 (Sigma-Aldrich). Absorbance was

(5) Real-world longitudinal assessment of anifrolumab in patients with systemic lupus erythematosus: clinical outcomes, safety and modulation of cytokines and neutrophil activityRMD OpenJune 28, 2026Alp Temiz, Rita Noversa de Sousa, Janina Schoen, Clara Reichardt, Kathrin Standfest, Andreas Wirsching, Melanie Hagen, Giulia Corte, Marco Muñoz Becerra, Koray Tascilar, et al.After 90 min incubation, plates were washed three times with PBS-T before adding 50 µL TMB substrate (#421101, BioLegend). The reaction was stopped after 60 min by adding 25% H2SO4 and measured at 450 nm with wavelength

(6) B cell TET2 recruits OGT for nuclear TET2 and H2B O-GlcNAcylation to drive AID and BLIMP-1 expression and maturation of the antibody responseCell ReportsJune 23, 2026Shili Li, Yibo Peng, Zhimin Yang, Xin Li, Zhenming Xu, Carlos E. Rivera, Paolo Casalidevelopment with O-phenylenediamine (OPD, P8806-50TAB, Sigma-Aldrich) or 3,3′,5,5′-Tetramethylbenzidine (TMB, 421101, BioLegend) substrate and measurement of converted substrate absorbance at 492 nm or 450 nm, respectively.

(7) Anti-CD38/Anti-CD20 Rescue Therapy for Post-Transplant Recurrent Focal Segmental GlomerulosclerosisKidney International ReportsJune 16, 2026Juliette Leon, Elise Ducrocq, Marie-Camille Lafargue, Dominique Bertrand, Lucie Maigret, Mohamad Zaidan, Arthur Michon Colin, Eric Thervet, Antoine Durrbach, Nassim Kamar, et al.room temperature with shaking (500 rpm). Following 5 additional washes, 100 μl/well of TMB substrate (421101, BioLegend) was added and allowed to react for 5 minutes at room temperature. The reaction was stopped

(8) HnRNP A1 and A2B1 enforce Ezh2 mRNA splicing to promote germinal center B cell responsesNature CommunicationsJune 11, 2026Zhijian Zhu, Rui Zhang, Haoran Kang, Meiyuan Chen, Hengjun Huang, Jing Wang, Ling Guo, Yuxing Li, Wenjing Chen, Limin Meng, et al.temperature, followed by three washes with PBST. The signal was developed using a TMB substrate (BioLegend, 421101), and the optical density at 450 nm (OD450) was measured using a microplate reader. Data analysis was

(9) Seasonal influenza mRNA vaccine induces stronger innate and comparable or better adaptive responses than licensed inactivated vaccinesNPJ VaccinesMay 26, 2026Erick Bermúdez-Méndez, Klara Lenart, Rodrigo Arcoverde Cerveira, Olivia Engstrand, Fredrika Hellgren, Alberto Cagigi, Sebastian Ols, Edith Jasny, Annika Reinhardt, Benjamin Petsch, et al.automatic plate washer. Signal was developed upon addition of TMB substrate solution (BioLegend, cat. no. 421101). The reaction was stopped after 7 min by addition of 1 M H2SO4. Absorbance was measured at 450 nm with

(10) Peroxisomal ABCD1 deficiency in mice drives Th1 bias through 25-HC–LXR signaling in CD4+ T cellsFrontiers in ImmunologyMay 20, 2026Reina Maeda, Mayu Oishi, Ryutaro Nagamori, Mari Hikosaka-Kuniishi, Shiro Watanabe, Jung-Bum Lee, Masashi Morita, Takanori Sodetection antibody. HRP-conjugated streptavidin (405210), and 3,3’,5,5’-tetramethylbenzidine substrate (421101) were also obtained from BioLegend. T cell restimulation assay Abcd1-deficient mice and wild-type

(11) Interleukin-4-producing type 2 innate lymphoid cells in the lymph node promote proallergic Tfh13 cell differentiationImmunityMay 19, 2026DongUk Lee, Meng-Ping Lu, Priya Rajamanimuthu, Mrinmoy Das, Enxhi Ferraj, Courtney Fisher, Peri Matatia, Chun-Wei Chen, Raif S. Geha, Caroline L. Sokol, et al.,Thermo Fisher Scientific,Cat# C34571 Fluoromount-G,Southern Biotech,Cat# 0100-01 TMB Substrate Set,Biolegend,Cat# 421101 MMCP-1 ELISA Kit,Thermo Fisher Scientific,Cat# 88-7503-22 Experimental models: Organisms/strains,Experimental models

(12) RNA binding protein Pcbp1 maintains mitochondria integrity to promote antibody production and germinal center responseSci AdvApril 10, 2026Lizhen Zhu, Li Lin, Tuan Qi, Geng Li, Zhixing Liang, Jiancheng Yu, Yifan Fu, Yue Peng, Xing Changincubation. For development, plates were incubated with trimethylboron (TMB) substrate (BioLegend, 421101) at room temperature in the dark for 1 to 5 min. The reaction was stopped with equal volume of 1 M sulfuric

(13) Identification of ovarian cancer-associated antigens forming complexes with autoantibodiesJournal of Ovarian ResearchApril 7, 2026Mimori Fujimori, Kai Uryu, Makoto Kobayashi, Kotaro Sugimoto, Yuta Endo, Shigenori Furukawa, Shu Soeda, Yoshio Kodera, Keiya Fujimori, Hideki Chibafor 1 h at 37℃. Signal development was performed using 3,3’,5,5’ tetramethyl benzidine substrate kit (421101, Biolegend, San Diego, CA). Absorbance at 490 nm was measured by Microplate Reader (AS ONE, Osaka, Japan)

(14) The therapeutic effects of the VEGF decoy receptor fusion protein VEGF-Grab in chronic inflammatory diseasesEBioMedicineApril 1, 2026Lee Y, Lee HY, Lim HS, Shin JA, Yang HG, Lee S, Mercadante G, Cicatiello V, De Falco S, Kim H, et al.p-nitrophenyl phosphate substrate in 0.1 M alkaline buffer, Sigma–Aldrich) or TMB substrate solution (catalog 421101; BioLegend) was added and incubated at RT for colour development. When sufficient colour development

(15) Strain-level antigen variation facilitates immune evasion in Bacteroides thetaiotaomicronJ ImmunolMarch 18, 2026Robert W P Glowacki, Jessica M Till, Orion D Brock, Vladimir Makarov, Morgan J Engelhart, Philip P Ahernthe addition of 50 µl of TMB substrate prepared as per the manufacturer’s instructions (BioLegend, 421101). The wells were visually monitored for color change and the ELISA was stopped by the addition of 50

(16) Generation of Aptamers for the Selective Detection and Neutralization of soluble Lymphotoxin alphaMolecular Therapy Nucleic AcidsMarch 12, 2026Matthew Stephens, Eman Nassef, Pierre-Yves von der von der Weidlight. Wells were washed again 3× with PBST before being incubated with TMB substrate (Biolegend Cat No. 421101) for 30 min in the dark. Th reaction was halted by addition of 0.1 M HCL, and 450 nm absorbance was

(17) All‐PEG‐Like Block Copolymers Self‐Assemble into Stealth Nanocarriers for Drug DeliveryAdvanced ScienceMarch 1, 2026Parul Sirohi, Brooke E. Silverstein, Yulia Shmidov, Sonal Deshpande, Joy Tong, Yvonne Y. Ma, Chinmay S. Potnis, Soumen Saha, Xinghai Li, Max R. Ney, et al.developed with 100 µL of pre‐mixed 3,3′,5,5′‐tetramethylbenzidine (TMB) liquid substrate (BioLegend # 421101) for 10 min at room temperature before stopping the reaction with the addition of 100 µL of Stop Solution

(18) IL‐2 silencing enables Tfh cell expansion during vaccination but is redundant for antibody productionImmunology & Cell BiologyFebruary 24, 2026Silvia Innocentin, Ross McKenzie, Jayalini Assalaarachchi, Helena A Carslaw, Anusha Gupta, Sanne Cole, Adrian Liston, Michelle A Linterman, Louise Webb, Alice R Burton(#ab97230, Abcam) antibody for 1 h at RT. In all cases, plates were developed with TMB substrate set (#421101, BioLegend) and the reaction stopped with 2.5 m H2SO4. Absorbance was measured with PHERAstar FS plate

(19) Recombinant Dabie bandavirus as a bivalent vaccine platform inducing protective immunity against intracellular pathogens and cancerMolecular TherapyFebruary 4, 2026Hyo-Jin Ro, Yebeen Lee, Kyeongseok Jeon, Yujin Kim, Seung Ho Baek, Green Kim, Joowan Kim, Jun-Gu Kang, Na-Yoon Jang, Si-Hyeon Lee, et al.#NB7215). After incubation, 3,3′,5,5′-tetramethylbenzidine (TMB) peroxidase substrate solution (BioLegend #421101) was added, and color development was allowed to proceed for 7 min. The reaction was stopped by adding

(20) FcγRIIB functions as an IgG transporter in the mammary glandProceedings of the National Academy of SciencesFebruary 3, 2026Xin Li, Lijuan Du, Zihui Wan, Zhiren Jin, Yuanhao Peng, Yongliang Fan, Cuncun Ke, Yonghe Ma, Haitang Han, Shuyang Yu, et al.temperature for 45 min and then wash the plate 5 times. Add 100 μL TMB Substrate Solution (BioLegend, 421101) to each well, incubate at room temperature in the dark for 13 min and then stop the reaction by adding

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TMB Substrate Set from BioLegend

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TMB Substrate Set from BioLegend

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