Alkaline Phosphatase Substrates

Alkaline Phosphatase Substrates Alkaline phosphatase (AP) substrates are used to facilitate the detection of protein targets in applications such as immunohistochemistry, western blotting, and ELISA. Some of the most widely used compounds include BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate along with nitro blue tetrazolium), pNPP (para-Nitrophenylphosphate), and Fast Red. These substrates produce a detectable color change when added to alkaline phosphatase labels, typically conjugated to antibodies. When choosing a proper AP substrate, consider the colors present in the final staining to ensure easy differentiation.

Alkaline Phosphatase Substrates

Your search terms and filters produced no results.

Please use the Alkaline Phosphatase Substrates search tool above to find what you're looking for.

Suggested Products

  • Millicell® DCI Digital Cell Imager

    Measure and assess your cell cultures with ease. Our Millicell® DCI Digital Cell Imager enables more efficient execution of the repetitive daily techniques associated with cell passaging. Quickly and objectively assess confluency, morphology, and growth trends for more consistent cell cultures. Expand capabilities with our off–device cloud storage and web-based application for data analysis, sorting, and archiving. Benefits Easy culture monitoring Faster analysis More accurate ...

  • MACSQuant® Analyzer 16 Flow Cytometer

    Discover the beauty of standardized and automated flow cytometry The MACSQuant® Analyzer 16 is an easy-to-use instrument designed for scalable and reliable flow cytometry assays. This includes features such as: Full workflow automation: sample processing, compensation, data acquisition, and analysis Easy assay transfer across multiple instruments and sites for comparable, standardized results Audit trail, analysis reports, and user management supporting 21 CFR Part 11 compliance and ...
  • <<
  • >>

Recent Product Review

  • Application Of The 3,3'-Diaminobenzidine (DAB) Substrate In ABC Immunohistochemical Staining

    The 14-3-3 (YWHA) proteins are a highly conserved, ubiquitously expressed family of proteins controlling important cellular processes, with seven known mammalian isoforms – beta, gamma, epsilon, zeta, eta, tau/theta and sigma. I examined the expression of 14-3-3 beta isoform in paraffin-embedded mouse ovary sections by ABC immunohistochemical staining, as part of studying the expression of the seven 14-3-3 isoforms. I selected the DAB substrate because it is known to generate distinct brown color upon reaction with the VectaStain Elite ABC reagent used in this method, and produces minimal background staining in absence of primary antibody. ABC immunohistochemistry detected 14-3-3 beta in ovarian follicles, with some similarities as well as notable differences in relative amounts, localizations and patterns of expression in multiple cell types at various stages of follicuogenesis. This study may help to elucidate isoform-specific protein interactions and functional roles of 14-3-3-beta in mammalian reproductive development.

  • Good chemiluminescent substrate for Western blot

    I use this chemiluminescent substrate to detect HRP conjugated secondary antibodies in order to detect proteins via Western blot. I used this chemiluminescent substrate because it is an easy two solution system that has consistently worked to detect HRP labeled secondary antibodies when I perform Western blots.