Sometimes It Works, Sometimes It Does Not

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Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

C12FDG (5-dodecanoylaminofluorescein di-beta-D-galactopyranoside)

Catalog Number:

D2893

Clone Number:

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C12FDG is a Beta-Gal substrate and can be used to access cell senescence. This probe can work very well or really bad depending on cell type. Works after bafilomycin incubation or depletion of CO2. It was tested in endothelial cells.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Human endothelial cells

Primary Incubation

33 uM

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fluorescence

Results Summary

This probe can work very well or really bad depending on cell type. Works after bafilomycin incubation or depletion of CO2.

DOI or PMID #

Additional Notes

Image Gallery

Summary

The Good

Can overcome the colorimetric staining for B-gal

The Bad

It does not always work

The Bottom Line

Easy to implement

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