Alkaline Phosphatase Substrates

Alkaline Phosphatase Substrates Alkaline phosphatase (AP) substrates are used to facilitate the detection of protein targets in applications such as immunohistochemistry, western blotting, and ELISA. Some of the most widely used compounds include BCIP/NBT (5-Bromo-4-chloro-3-indolyl phosphate along with nitro blue tetrazolium), pNPP (para-Nitrophenylphosphate), and Fast Red. These substrates produce a detectable color change when added to alkaline phosphatase labels, typically conjugated to antibodies. When choosing a proper AP substrate, consider the colors present in the final staining to ensure easy differentiation.

Alkaline Phosphatase Substrates

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MyBioSource.com

BCIP/NBT Substrate

BCIP/NBT Substrate
  • Quantity:
    100 ml
  • Applications:
    ELISA, Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Supplier Page$170.00
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Abcam

4-Aminophenyl phosphate monosodium salt, Alkaline phosphatase substrate

4-Aminophenyl phosphate monosodium salt, Alkaline phosphatase substrate
  • Quantity:
    10 mg, 50 mg
  • Detection Method:
    Colorimetric
  • Format:
    Solid
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BCIP disodium salt, histochemical alkaline phosphatase substrate

BCIP disodium salt, histochemical alkaline phosphatase substrate
  • Quantity:
    100 mg, 500 mg
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Citations:
(2)
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Enzo Life Sciences, Inc.

pNpp substrate, (100 ml)

pNpp substrate, (100 ml)
  • Quantity:
    100 ml
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
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Rockland Immunochemicals, Inc.

BCIP/NBT Membrane Alkaline Phosphatase Substrate

BCIP/NBT Membrane Alkaline Phosphatase Substrate
  • Quantity:
    100 mL
  • Applications:
    Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Citations:
(3)
Supplier Page$132.00
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AAT Bioquest, Inc.

pNPP [4-Nitrophenyl phosphate, disodium salt]

pNPP [4-Nitrophenyl phosphate, disodium salt]
  • Quantity:
    25 mg
Supplier Page$86.00
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DiFMUP

DiFMUP
  • Quantity:
    5 mg
  • Detection Method:
    Fluorometric
Citations:
(3)
Supplier Page$109.00
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MyBioSource.com

pNPP

pNPP
  • Quantity:
    100 ml
  • Applications:
    ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Supplier Page$195.00
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Nuclear Fast Red

Nuclear Fast Red
  • Quantity:
    12 ml
  • Applications:
    Immunohistochemistry
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Supplier Page$90.00
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Novus Biologicals, a Bio-Techne Brand

AutoRED AP Plus Staining Kit (Colorimetric)

AutoRED AP Plus Staining Kit (Colorimetric)
  • Quantity:
    30 ml
  • Applications:
    In Situ Hybridization, Immunohistochemistry, Immunohistochemistry - fixed
  • Detection Method:
    Colorimetric
Supplier Page$229.00
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AutoRED AP Staining Kit (Colorimetric)

AutoRED AP Staining Kit (Colorimetric)
  • Quantity:
    30 ml
  • Applications:
    In Situ Hybridization, Immunohistochemistry, Immunohistochemistry - fixed
  • Detection Method:
    Colorimetric
Supplier Page$179.00
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Bio-Rad

pNPP

pNPP
  • Quantity:
    500 ml
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Supplier Page$597.00
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pNPP

pNPP
  • Quantity:
    100 ml
  • Applications:
    EIA, Immunoassay, ELISA
  • Detection Method:
    Colorimetric
  • Format:
    Liquid
Supplier Page$138.00
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Neogen Corporation

Chemiluminescent AP Select 450

Chemiluminescent AP Select 450
  • Quantity:
    200 mL
  • Applications:
    Immunoassay
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
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Chemiluminescent AP Select 450

Chemiluminescent AP Select 450
  • Quantity:
    1 Liter
  • Applications:
    Immunoassay
  • Detection Method:
    Chemiluminescent
  • Format:
    Liquid
Supplier Page
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Biomatik

BCIP, Toluidine Salt (BCIP-T)

BCIP, Toluidine Salt (BCIP-T)
  • Quantity:
    100MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Supplier Page$39.00
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Nitro Blue Tetrazolium Chloride [NBT]

Nitro Blue Tetrazolium Chloride [NBT]
  • Quantity:
    250MG
  • Applications:
    Immunohistochemistry, Western Blot
  • Detection Method:
    Colorimetric
  • Format:
    Solid
Supplier Page$25.00
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Promega

AttoPhos AP Fluorescent Substrate System

AttoPhos AP Fluorescent Substrate System
  • Quantity:
    3 x 36mg
  • Detection Method:
    Fluorometric
  • Format:
    Solid
Citations:
(50)
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AttoPhos AP Fluorescent Substrate System Trial Size

AttoPhos AP Fluorescent Substrate System Trial Size
  • Quantity:
    1 x 36mg
  • Detection Method:
    Fluorometric
  • Format:
    Solid
Citations:
(6)
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Showing 10 of 14 suppliers (52 products total)

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