Deproteinizing Sample Preparation Kit - TCA ab204708 from Abcam

Product Specs
ItemDeproteinizing Sample Preparation Kit - TCA
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204708
Quantity200 Test
Sample TypeUrine, Whole Blood, Tissue Extracts, Tissue Culture Media, Tissue Lysate
Assay Time0h 25m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(61) Multivalent Ligand-Nanoparticle Conjugates Amplify Reactive Oxygen Species Second Messenger Generation and Enhance Epidermal Growth Factor Receptor PhosphorylationBioconjugate ChemistrySeptember 21, 2022Sandy Zhang, Tianhong Ouyang, Björn M. Reinhardcell lysates. Lysates were deproteinized using the Deproteinizing sample preparation kit – TCA (Abcam, ab204708) following the manufacturer’s protocol. After deproteinization, the samples were ready to use. After

(62) Downregulation of Elovl5 promotes breast cancer metastasis through a lipid-droplet accumulation-mediated induction of TGF-β receptorsCell death & diseaseSeptember 2, 2022Kieu TL, Pierre L, Derangère V, Perrey S, Truntzer C, Jalil A, Causse S, Groetz E, Dumont A, Guyard L, et al.quantification Total cell lysates were collected and deproteinized using the Deproteinization kit (#ab204708; Abcam). Intracellular Acetyl-CoA content was determined using the Acetyl-CoA assay kit (#PicoProbe

(63) Mitochondrial uncoupling protein‐2 reprograms metabolism to induce oxidative stress and myofibroblast senescence in age‐associated lung fibrosisAging CellSeptember 1, 2022Sunad Rangarajan et al.fractions were collected and subjected to the deproteinization procedure via TCA precipitation (Abcam; ab204708). The deproteinized samples were then incubated with required reaction components for 30 min in the

(64) The hexokinase “HKDC1” interaction with the mitochondria is essential for liver cancer progressionCell Death & DiseaseJuly 28, 2022Md. Wasim Khan, Alexander R. Terry, Medha Priyadarshini, Vladimir Ilievski, Zeenat Farooq, Grace Guzman, Jose Cordoba-Chacon, Issam Ben-Sahra, Barton Wicksteed, Brian T. Laydensupernatants were then collected and deproteinization was done using Abcam Sample preparation kit (ab204708). Deproteinized samples were incubated with the ATP probe as per manufacturer’s recommendations. Absorbance

(65) Differential Induction of Reactive Oxygen Species and Expression of Antioxidant Enzymes in Human Melanocytes Correlate with Melanin Content: Implications on the Response to Solar UV and Melanoma SusceptibilityAntioxidants (Basel)June 20, 2022Parth R. Upadhyay, Renny J. Starner, Viki B. Swope, Kazumasa Wakamatsu, Shosuke Ito, Zalfa A. Abdel-Maleklysates were then deproteinized and neutralized using a deproteinizing sample preparation kit (Cat. No. ab204708; Abcam, Boston, MA, USA). A GSH/GSSG ratio detection kit (Cat. No. ab205811; Abcam, Boston, MA, USA)

(66) Let-7 underlies metformin-induced inhibition of hepatic glucose productionProceedings of the National Academy of SciencesApril 5, 2022Di Xie et al.ice-cold tubes and treated with Deproteinizing Sample Preparation Kit with trichloroacetic acid (Abcam, ab204708) to remove enzymes that could interfere with the analysis. For GSH detection, 50 μL of fresh GSH Assay

(67) Enhanced RAGE Expression and Excess Reactive-Oxygen Species Production Mediates Rho Kinase-Dependent Detrusor Overactivity After Methylglyoxal ExposureFrontiers in PhysiologyMarch 28, 2022Akila L. Oliveira, Matheus L. Medeiros, Mariana G. de Oliveira, Caio Jordão Teixeira, Fabíola Z. Mónica, Edson Antuneslevels in serum, the samples were deproteinized using Deproteinizing Sample Preparation Kit - TCA (Abcam ab204708) according to the manufacturer’s instructions. Serum levels of MGO were measured using ELISA competitive

(68) Oxidative stress and genotoxicity in 1,4-dioxane liver toxicity as evidenced in a mouse model of glutathione deficiencyScience of The Total EnvironmentFebruary 1, 2022Ying Chen, Yewei Wang, Georgia Charkoftaki, David J. Orlicky, Emily Davidson, Fengjie Wan, Gary Ginsberg, David C. Thompson, Vasilis Vasiliouand oxidized glutathione (GSSG) fluorometric assay kit (ab138881) and the Deproteinizing Sample Kit (ab204708) were purchased from Abcam (Waltham, MA, USA). The 8-OHdG competitive ELISA assay kit was purchased

(69) Metabolic Reprogramming of Glioblastoma Cells during HCMV Infection Induces Secretome-Mediated Paracrine Effects in the MicroenvironmentVirusesJanuary 7, 2022Harrison MAA, Hochreiner EM, Benjamin BP, Lawler SE, Zwezdaryk KJ.Supernatant and cell lysates were deproteinized with TCA following the manufacturer’s instructions (#ab204708; Abcam). Lactate concentration was determined by comparison with standards using an L-lactate assay

(70) Ionophore Ability of Carnosine and Its Trehalose Conjugate Assists Copper Signal in Triggering Brain-Derived Neurotrophic Factor and Vascular Endothelial Growth Factor Activation In VitroInternational journal of molecular sciencesDecember 16, 2021Naletova I, Greco V, Sciuto S, Attanasio F, Rizzarelli E.min at 14,000 r.p.m.). Supernatants were treated by deproteinizing Sample Preparation Kit–TCA (Cat#ab204708, Abcam, Waltham, MA, USA) and were then collected and processed according to the kit protocol (excitation/emission

(71) Methylglyoxal Exacerbates Lipopolysaccharide-Induced Acute Lung Injury via RAGE-Induced ROS Generation: Protective Effects of MetforminJ Inflamm ResDecember 2, 2021Matheus L Medeiros, Akila L Oliveira, Mariana G de Oliveira, Fabíola Z Mónica, Edson Antunesin serum, samples were deproteinized using the Deproteinizing Sample Preparation Kit – TCA (Cat. No. Ab204708, Abcam, Cambridge, UK) following the manufacturer’s instructions, and then quantified using an ELISA

(72) Methylglyoxal Exacerbates Lipopolysaccharide-Induced Acute Lung Injury via RAGE-Induced ROS Generation: Protective Effects of MetforminJournal of Inflammation ResearchDecember 2, 2021Matheus L Medeiros, Akila L Oliveira, Mariana G de Oliveira, Fabíola Z Mónica, Edson Antunesin serum, samples were deproteinized using the Deproteinizing Sample Preparation Kit – TCA (Cat. No. Ab204708, Abcam, Cambridge, UK) following the manufacturer’s instructions, and then quantified using an ELISA

(73) Novel rat model of multiple mitochondrial dysfunction syndromes (MMDS) complicated with cardiomyopathyAnimal models and experimental medicineDecember 1, 2021Ling Y, Ma J, Qi X, Zhang X, Kong Q, Guan F, Dong W, Chen W, Gao S, Gao X, et al.µl of ATP assay buffer and deproteinized using a deproteinizing sample preparation kit (UK, Abcam, ab204708). The standard curve was prepared based on the colorimetric assay and the absorbance was assayed in

(74) The function of Scox in glial cells is essential for locomotive ability in DrosophilaScientific ReportsDecember 1, 2021Ryosuke Kowada, Atsushi Kodani, Hiroyuki Ida, Masamitsu Yamaguchi, Im-Soon Lee, Yasushi Okada, Hideki YoshidaAbcam), centrifuged, and the supernatant was collected. Then, 10 µl of chilled trichloroacetic acid (ab204708, Abcam) was mixed with supernatants and samples were reacted at 4 °C for 15 min. After centrifugation,

(75) Neuroprotective effects of Withania somnifera in the SH-SY5Y Parkinson cell modelHeliyonOctober 13, 2021Jeerang Wongtrakul, Thananya Thongtan, Benjawan Kumrapich, Chonticha Saisawang, Albert J. Kettermaninsoluble materials. The supernatants were collected, transferred to new tubes and deproteinized using Deproteinizing Sample Preparation Kit – TCA (Abcam, UK). Briefly, 10 μl of cold TCA was added into 100 μl sample. The sample tube was placed on

(76) Distinct pro-inflammatory properties of myeloid cell-derived apolipoprotein E2 and E4 in atherosclerosis promotionThe Journal of biological chemistrySeptember 1, 2021Igel E, Haller A, Wolfkiel PR, Orr-Asman M, Jaeschke A, Hui DY.ice-cold 0.5% NP-40 in PBS. Samples were deproteinated using a trichloroacetic acid precipitation kit (ab204708, Abcam). Intracellular GSH and hydrogen peroxide levels were measured using fluorescent assay kits (ab205811

(77) Multiplexed proteomics of autophagy-deficient murine macrophages reveals enhanced antimicrobial immunity via the oxidative stress responseeLifeJune 4, 2021Maculins T, Verschueren E, Hinkle T, Choi M, Chang P, Chalouni C, Rao S, Kwon Y, Lim J, Katakam AK, et al.transferred to a fresh tube and used for deproteinization following manufacturer's instructions (Abcam, ab204708). The resultant supernatant was used for determining reduced GSH, total GSH and oxidized GSSG was calculated

(78) The pentose phosphate pathway mediates hyperoxia-induced lung vascular dysgenesis and alveolar simplification in neonatesJCI insightMarch 8, 2021Gong J, Feng Z, Peterson AL, Carr JF, Lu X, Zhao H, Ji X, Zhao YY, De Paepe ME, Dennery PA, et al.manufacturer’s instructions. All samples were deproteinized with the Deproteinizing Sample Preparation Kit-TCA (ab204708, Abcam) before analysis. Protein concentration in samples before deproteinization was measured for normalization. Determination

(79) Elevated Lactate by High-Intensity Interval Training Regulates the Hippocampal BDNF Expression and the Mitochondrial Quality Control SystemFrontiers in physiologyJanuary 1, 2021Hu J, Cai M, Shang Q, Li Z, Feng Y, Liu B, Xue X, Lou S.L-lactate assay kit (No. ab65331, Abcam) according to the manufacturer’s protocol. We used TCA Kit (No. ab204708, Abcam) to remove enzyme from tissue sample in case of endogenous LDH degrading lactate. HIIT Paradigm Mice

(80) Oxidative Phosphorylation Is Dysregulated Within the Basocortical Circuit in a 6-month old Mouse Model of Down Syndrome and Alzheimer's DiseaseFrontiers in aging neuroscienceJanuary 1, 2021Alldred MJ, Lee SH, Stutzmann GE, Ginsberg SD.Deproteinization of mouse Fr Ctx homogenates were performed utilizing the Deproteinizing Sample preparation kit (ab204708, AbCam) according to manufacturer’s specifications with the following alterations, starting sample volume

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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