Deproteinizing Sample Preparation Kit - TCA ab204708 from Abcam

Product Specs
ItemDeproteinizing Sample Preparation Kit - TCA
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab204708
Quantity200 Test
Sample TypeUrine, Whole Blood, Tissue Extracts, Tissue Culture Media, Tissue Lysate
Assay Time0h 25m

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(21) Neuronal glycogen breakdown mitigates tauopathy via pentose-phosphate-pathway-mediated oxidative stress reductionNature MetabolismJune 30, 2025Sudipta Bar, Kenneth A. Wilson, Tyler A. U. Hilsabeck, Sydney Alderfer, Eric B. Dammer, Jordan B. Burton, Samah Shah, Anja Holtz, Enrique M. Carrera, Jennifer N. Beck, et al.tissue lysate was used for the assay. Deproteinization was performed using a kit from Abcam (cat. no. ab204708). PKA activity was measured using a kit protocol (Thermo Fisher cat. no. EIAPKA). For PKA activity,

(22) The RAGE Inhibitor TTP488 (Azeliragon) Improves Diabetic Bladder Dysfunction in Leptin-Deficient Obese MiceAntioxidants (Basel)June 27, 2025Akila Lara Oliveira, Matheus Leite Medeiros, Antonio Thiago Pereira Campos, Carlos Lenz Cesar, Fabiola Zakia Mónica, Edson Antunespreviously deproteinized using a Deproteinizing Sample Preparation Kit (TCA, Abcam, Cambridge, UK, ab204708), according to the manufacturer’s instructions. Levels of MG-H1 were measured in deproteinized samples

(23) PCSK9 deficiency promotes the development of peripheral neuropathyJCI InsightJune 23, 2025Ali K. Jaafar, Aurélie Paulo-Ramos, Guillaume Rastoldo, Bryan Veeren, Cynthia Planesse, Matthieu Bringart, Philippe Rondeau, Kévin Chemello, Olivier Meilhac, Gilles C. Lambert, et al.signals at 570 nm, samples were deproteinized with a Deproteinizing Sample Preparation Kit (Abcam, ab204708). Protein extracts were obtained after lysis of tissues in 350-μL Tris HCl buffer (pH 9.5; 10 mM) containing

(24) Liposomal Nanoparticle Delivery of Ginkgo Flavone Glycosides Enhances SIRT1 Activation and Improves Diabetic CardiomyopathyInt J NanomedicineJune 9, 2025Wei Gao, Ruoran Chen, Huixin Tong, Yu Wang, Linlang Liang, Yiyao Li, Hao Yufor 3–6 hours. After treatment, cell lysates were prepared using a protein extraction reagent (Abcam, ab204708). The ATP content in the lysates was measured by the generation of a product detectable by fluorescence

(25) Liposomal Nanoparticle Delivery of Ginkgo Flavone Glycosides Enhances SIRT1 Activation and Improves Diabetic CardiomyopathyInt J NanomedicineJune 9, 2025Wei Gao, Ruoran Chen, Huixin Tong, Yu Wang, Linlang Liang, Yiyao Li, Hao Yufor 3–6 hours. After treatment, cell lysates were prepared using a protein extraction reagent (Abcam, ab204708). The ATP content in the lysates was measured by the generation of a product detectable by fluorescence

(26) Novel Pt@PCN-Cu-induced cuproptosis amplifies αPD-L1 immunotherapy in pancreatic ductal adenocarcinoma through mitochondrial HK2-mediated PD-L1 upregulationJ Exp Clin Cancer ResMay 17, 2025Pengyu Wang, Weihua Guo, Shuyue Liu, Shouyi Li, Jiaqi Li, Bowen Ding, Fengyi Yin, Yang Yang, Xingjiang Li, Pei Cao, et al.collect the supernatant, which was deproteinized using the deproteinizing sample preparation kit-TCA (ab204708, Abcam, U.K.). After adding the reaction mix, the samples were incubated at room temperature for 30

(27) Deoxynucleoside supplementation ameliorates the disease associated phenotypes in a zebrafish model of RRM2B mtDNA depletion syndromeHuman Molecular GeneticsMay 17, 2025Benjamin Munro, Declan Hines, Juliane S Mueller, Rita Horvathusing the L-Lactate Assay Kit (Abcam, ab65330) with the Deproteinizing Sample Preparation Kit—TCA (ab204708). Statistical analysis All statistical analysis was performed in Graph Pad Prism v8.4.3 (more details

(28) Exercise-Induced Hippocampal Neurogenesis Is Attenuated by Inhibition of Monocarboxylate Transporter 2Molecular NeurobiologyMay 8, 2025Deunsol Hwang, Taeho Kim, Sunghwan Kyun, Inkwon Jang, Jisu Kim, Hun-Young Park, Sung-Woo Kim, Kiwon Limassay, all samples were deproteinized using a commercial Deproteinizing Sample Preparation Kit-TCA (ab204708; Abcam) to remove endogenous lactate dehydrogenase (an enzyme that degrades lactate). Immunofluorescence One

(29) In vitro study on the promotion of osteogenic differentiation by mitochondrial-derived vesicles through activation of inflammation and reprogramming of metabolic pathwaysJ Orthop Surg ResApril 17, 2025Chun Pan, Cheng Cheng, Shu Zhong, Shiyu Li, Wei Tan, Yachao Yaoother proteins, the supernatants were deproteinized using the Deproteinizing Sample Preparation Kit (ab204708, Abcam). The deproteinized samples were stored on ice until further analysis. A standard curve was prepared

(30) Housing Temperature Impacts the Systemic and Tissue-Specific Molecular Responses to Cancer in MiceJ Cachexia Sarcopenia MuscleApril 16, 2025Andrea Irazoki, Emma Frank, Tang Cam Phung Pham, Jessica L. Braun, Amy M. Ehrlich, Mark Haid, Fabien Riols, Camilla Hartmann Friis Hansen, Anne-Sofie Rydal Jørgensen, Nicoline Resen Andersen, et al.Determination For the ATP determination assay, the deproteinizing sample preparation kit—TCA (Abcam, ab204708) was used on pulverized BAT and triceps muscles to remove interfering macromolecules. Homogenized tissues

(31) Metabolic changes during cardiac regeneration in the axolotlDevelopmental DynamicsMarch 22, 2025Anita Dittrich, Sofie Amalie Andersson, Morten Busk, Kasper Hansen, Casper Bindzus Foldager, Johan Palmfeldt, Asger Andersen, Michael Pedersen, Mikkel Vendelbo, Kirstine Lykke Nielsen, et al.−80°C. Prior to the microplate assay, samples were deproteinized using a commercial kit (Abcam; Cat.nr: ab204708). Lactate was measured in 5 μL samples diluted to a total volume of 100 μL using a commercial kit (Sigma

(32) Pten Loss Triggers Progressive Photoreceptor Degeneration in an mTORC1-Independent MannerInvest Ophthalmol Vis SciMarch 21, 2025Joseph Hanna, Yacine Touahri, Alissa Pak, Luke Ajay David, Edwin van Oosten, Rajiv Dixit, Laura M. Vecchio, Dhruv Nimesh Mehta, Ren Minamisono, Isabelle Aubert, et al.dehydrogenase in the sample, which might degrade lactate using the Deproteinizing Sample Preparation Kit – TCA (ab204708; Abcam). Measurements of lactate were then performed using an L-Lactate Assay Kit (Colorimetric/Fluorometric)

(33) Plasma extracellular vesicles from recurrent GBMs carrying LDHA to activate glioblastoma stemness by enhancing glycolysisTheranosticsFebruary 26, 2025Xin Zhang, JunJie Li, Yiyao Huang, Anming Yang, Xiaoliu Liu, Yunhao Luo, Hao Tian, Minghui Wen, Chengzong Zhong, Bin Peng, et al.The endogenous LDH was removed from the sample using the Deproteinizing Sample Preparation Kit-TCA (ab204708). A commercial L-Lactate Assay Kit (Abcam, Cambridge, UK, ab65330) was used according to the manufacturer's

(34) Combination of Low-Dose Sulforaphane and Docetaxel on Mitochondrial Function and Metabolic Reprogramming in Prostate Cancer Cell LinesInternational Journal of Molecular SciencesJanuary 24, 2025Ana Peñata-Taborda, Pedro Espitia-Pérez, Lyda Espitia-Pérez, Andrés Coneo-Pretelt, Hugo Brango, Dina Ricardo-Caldera, Gean Arteaga-Arroyo, Luisa Jiménez-Vidal, Claudia Galeano-Páez, Karina Pastor-Sierra, et al.cells were collected during the treatment time and deproteinized using the Deproteinization-TCA kit (ab204708, Abcam, Cambridge, UK) and Halt™ protease inhibition cocktail, EDTA-free (Thermo Fisher Scientific,

(35) PDCD6 regulates lactate metabolism to modulate LC3-associated phagocytosis and antibacterial defenseNature CommunicationsNovember 23, 2024Lulu Sun, Sijin Wu, Hui Wang, Tianyu Zhang, Mengyu Zhang, Xuepeng Bai, Xiumei Zhang, Bingqing Li, Cai Zhang, Yan Li, et al.homogenized in 100 μL of lactate assay buffer with a G26 needle and deprotonated using TCA (Abcam, ab204708). The lactic acid content was calculated by measuring the OD at 530 nm. LDH activity assay The LDH

(36) A Skeletal Muscle-Mediated Anticontractile Response on Vascular Tone: Unraveling the Lactate-AMPK-NOS1 Pathway in Femoral ArteriesFunction (Oxf)November 20, 2024Milene T Fontes, Tiago J Costa, Ricardo B de Paula, Fênix A Araújo, Paula R Barros, Paul Townsend, Landon Butler, Kandy T Velazquez, Fiona Hollis, Gisele F Bomfim, et al.(ab65331, Abcam). All samples were deproteinized with the Deproteinizing Sample Preparation Kit-TCA (ab204708, Abcam). The protein concentration was measured using the BCA protein assay kit (ThermoFisher Scientific,

(37) Antisense Oligonucleotide STK-002 Increases OPA1 in Retina and Improves Mitochondrial Function in Autosomal Dominant Optic Atrophy CellsNucleic Acid TherapeuticsOctober 16, 2024Aditya Venkatesh, Taylor McKenty, Syed Ali, Donna Sonntag, Shobha Ravipaty, Yanyan Cui, Deirdre Slate, Qian Lin, Anne Christiansen, Sarah Jacobson, et al.protein concentration. The remaining sample was deproteinized using the Deproteinizing TCA Kit (Abcam, ab204708) and ATP levels were measured in the lysates using the ATP Assay Kit (Abcam, ab83355) according to the

(38) A lactate metabolism-related gene signature to diagnose osteoarthritis based on machine learning combined with experimental validationAging (Albany NY)October 16, 2024Jianhua Yang, Wenjun Li, Xuemei Lin, Wei Liangassay buffer (Abcam, The Netherlands) and the endogenous lactate dehydrogenase was removed using the deproteinizing sample preparation kit-TCA (Abcam, The Netherlands). The lactate concentration of the samples was then determined using a microplate

(39) Frequency-Dependent Antioxidant Responses in HT-1080 Human Fibrosarcoma Cells Exposed to Weak Radio Frequency FieldsAntioxidants (Basel)October 15, 2024Hakki Gurhan, Frank Barnesice. Deproteinization of cell lysates was performed using the Deproteinizing Sample Preparation Kit—TCA (Cat. No. ab204708, Abcam, Cambridge, UK). For samples with protein concentrations less than 25 mg/mL, 150 µL of the sample

(40) Stress triggers gut dysbiosis via CRH-CRHR1-mitochondria pathwaynpj Biofilms and MicrobiomesSeptember 30, 2024Yiming Zhang, Xiaoang Li, Siqi Lu, Huaizhu Guo, Zhuangyi Zhang, Haonan Zheng, Cunzheng Zhang, Jindong Zhang, Kun Wang, Fei Pei, et al.epithelium was isolated and then deproteinized using the Deproteinizing Sample Preparation Kit (Abcam, ab204708) following the manufacturer’s instructions. Lactate measurement in the colon epithelium lysates was

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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Deproteinizing Sample Preparation Kit - TCA from Abcam

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